ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 10 (2012), No 1, p 29-34
DOI 10.2478/v10136-011-0019-y

The role of the light/dark cycle in the daily rhythm of serum proteins in Equus caballus

Giuseppe Piccione, Simona Marafioti, Claudia Giannetto, Caterina Faggio, Michele Panzera, Francesco Fazio

Address: Giuseppe Piccione, Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Polo Universitario dell'Annunziata, 98168, Messina, Italy
giuseppe.piccione@unime.it

Received 23rd May 2011.
Revised 2nd September 2011.
Published online 3rd November 2011.

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SUMMARY
This research was carried out on five clinically healthy Sella Italiana horses to determine the daily rhythm of total proteins and their fractions, to establish if these rhythms are endogenously generated and to assess the role of light as synchroniser of these rhythms. Blood samples were collected from each subject every 3 h over a period of 48 h, starting at 9:00 on day 1 and finishing at 9:00 on day 3, into vacutainer tubes without an anticoagulant via intravenous cannulas inserted into the jugular vein. Total serum proteins, albumin, alpha1-, alpha2-, beta1-, beta2- and gamma- globulin concentrations were assessed in all samples. The application of two-way ANOVA showed a significant effect of the time of day on total proteins, albumin, beta1- and beta2- globulins, and of the experimental conditions on total proteins alpha1-, alpha2-, beta1-, beta2- and gamma- globulins. No statistical modifications were observed on the A/G ratio. Daily rhythmicity was exhibited only by total proteins and albumin during the L/D cycle. We can claim that the fluctuation of serum total proteins and albumin concentrations are daily and not circadian and that they are driven by the L/D cycle.

KEY WORDS
total proteins; electrophoresis profile; daily rhythm; horse; photoperiod

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CITED

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