ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 11 (2013), No 4, p 223-233
DOI 10.2478/v10136-012-0025-8

Nitrite contamination in hypotensive preparations of dinitrosyl iron complexes with glutathione

Rostislav Romanovich Borodulin, Vyacheslav Olegovich Shvydkiy, Lyudmila Nikolaevna Kubrina, Vasak Dzhanibekovich Mikoyan, Natalya Ilinishna Zaitseva, Tatyana Evgenjevna Sporyagina, Sergey Alexandrovich Kryuchkov, Anatoly Fjodorovich Vanin

Address: Anatoly Fjodorovich Vanin, 119991, Kosygin Str. 4, Chemical Physics Institute, Moscow, Russia

Received 19th October 2012.
Revised 14th November 2012.
Published online 15th November 2012.

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The content of nitrite admixture in preparations of dinitrosyl iron complexes (DNIC) with glutathione synthesized by treatment of aqueous solutions of Fe2+ + glutathione with gaseous NO (complex 1) or by mixing solutions of S-nitrosoglutathione (GS-NO) with solutions of Fe2+ + glutathione (complex 2) was determined using the Griess method and HPLC as well as from the level of HNO2 formed upon interaction of gaseous NO with acidified distilled water. In both preparations, DNIC were predominantly represented by the binuclear form (B-DNIC). In complex 1, the appearance of nitrite in DNIC solutions was induced by nitrogen dioxide present in gaseous NO; its interaction with NO gives an adduct, which is further hydrolyzed to nitrite in aqueous solutions. In complex 2, the presence of nitrite admixture could appear in the presence of nitrite non-incorporated into GS-NO synthesized by mixing glutathione and nitrite in acid media. The per cent content of nitrite (with respect to the total content of complex 1) was 6%, whereas in complex 2 it was as low as 0.4%. Such a low level of nitrite contamination in the course of conventional synthesis of DNIC with glutathione does not make any significant contribution to their biomedical (e.g., hypotensive or vasodilator) activity.

dinitrosyl iron complexes; nitrite; high-performance liquid chromatography; Griess method

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