Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 1 (2003), No 2, p 99-106




Capillary electrophoresis determination of huperzine A: A new prospective anti-dementia drug

Eladia M. Pena-Mendez, Tomas Hottmar, Josef Havel, Jiri Patocka

Address: Jiri Patocka, Department of Toxicology, Military Medical Academy, Simkova 878, 500 01 Hradec Kralove, Czech Republic
patocka@pmfhk.cz

Received 10th September 2002.
Published online 28th February 2003.

Full text article (pdf)

SUMMARY
Huperzine A is an alkaloid isolated from the Chinese plant Huperzia serrata. The absorption spectra and electrophoretic behaviour of the compound were studied. The compound shows two absorption maxima (lambdamax 230 and 310nm). The protonation constant was determined by spectrophotometry and capillary zone electrophoresis (CZE) and the recommended value is pK=7.70±0.07. A CZE method for the determination of Huperzine A in pharmaceutical tablets and serum has been developed. The method is based on separation of the compound as a protonated species with electrophoretic mobility determined as 50.7 x 10-9 m2V-1s-1. The optimal separation conditions were: acetate buffer (pH 4.60; 20 mM) as a background electrolyte, separation voltage 20 kV, temperature 25 °C, hydrodynamic injection 10 s and detection at wavelengths 230 and 310 nm. The analyses were run in an uncoated fused-silica capillary with total length of 47.5 cm (39.0 cm to the detector) and 75 microm I.D. The detection limit was found to be 70 microg/l. The method of Huperzine A determination was applied for analysis of various pharmaceutical products. The results were found to be in good agreement with the declared amount. The precision of the method is ±2.30%. A method for the determination of HupA in serum was also developed with detection limit 110 microg/l, which is applicable for pharmacokinetics and therapeutic drug monitoring of this compound.

KEY WORDS
huperzine A; Alzheimer's disease; capillary zone electrophoresis


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