ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 1 (2003), No 2, p 93-97

Are nucleoli participating in programmed cell death

Karel Smetana

Address: Karel Smetana, Institute of Hematology and Blood Transfusion, U nemocnice 1, 128 20 Praha 2, Czech Republic

Received 11th December 2002.
Published online 4th March 2003.

Full text article (pdf)

Despite numerous publications on nucleoli, their participation in terminal maturation and programmed cell death have not been studied extensively. On the other hand, some observations clearly indicate that terminal maturation and programmed cell death are accompanied by marked nucleolar changes which reflect the cessation of nucleolar biosynthetic activities. In addition, nucleolar changes in the course of terminal maturation may precede programmed cell death.

nucleolus; erythrocytic and granulocytic precursors; terminal maturation; programmed cell death

Bessis M: Living Blood Cells and Their Ultrastructure. Springer, Berlin, 1973.

Biggiogera M, Pelliciari C: Heterogeneous ectopic RNP derived structures (HERDS) are markers of transcriptional arrest. FASEB J 14:828-834, 2000.

Busch H: Nucleolar and nucleolonemal proteins of cancer cells. J. Tumor Marker Oncol 12:5-68, 1997.

Busch H, Smetana K: The nucleolus. Academic Press, New York, 1970.

Chan PK, Chan FY: A study of correlation between NPM-translocation and apoptosis in cells induced by Daunomycin. Biochem Pharmacol 57:1265-1273, 1999.

Derenzini M: The AgNORs. Micron 31:117-1120, 2000.

Hozak P, Cook PR, Schofer C, Mosgoller W, Wachtler F: Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J Cell Sci 107:639-678, 1994.

Koberna K, Malinsky J, Pliss A, Masata M, Vecerova J, Fialova M, Bednar J, Raska I: Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of Christmas trees. J Cell Biol 157:743-748, 2002.

Likovsky Z, Smetana K: Further studies on the cytochemistry of the standardized silver staining of interfuse nucleoli in smear preparations of Yoshida ascetic sarcoma cells in rats. Histochemistry 72:301-313, 1981.

Likovsky Z, Smetana K: Nucleolar coefficient of granulocyte precursors and granulocytes after visualization of nucleoli by two different methods. Acta Histochem 102:95-102, 2000.

Martelli AM, Zweyer M, Ochs RL, Tazzari PL, Tabellini G, Narducci P, Bortul R: Nuclear apoptotic changes: An overview. J Cell Biochem 82:634-646, 2001.

Melcak I, Risueno MC, Raska I: Ultrastructural nonisotopic mapping of nuclear transcription sites in onion protoplasts. J Struct Biol 116:253-263, 1996.

Morimoto Y, Kito S, Ohba T, Morimoto H, Okamura H, Haneji T: Alteration of aryrophilic nucleolar organizer region associate (AgNOR) proteins in apoptosis - induced human salivary gland and human squamous carcinoma cells. J Oral Pathol Med 30: 193-199, 2001.

Ochs RL, Smetana K: Detection of fibrillarin in nucleolar remnants and the nucleolar matrix. Exp Cell Res 197:183-190, 1991.

Pederson T: The plurifunctional nucleolus. Nucleic Acids Res 26:3871-3876, 1998.

Scott M, Boisvert FM, Vieyera FM, Johnston RN, Bazett Jones DP, Riabowol K: UV induces nucleolar translocation of ING1 through two distinct nucleolar targeting. Nucl Acids Res 29:2052-2058, 2001.

Schwarzacher HG, Wachtler F: Nucleolus organizer regions in nucleoli. Hum Genet 63:89-99, 1983.

Smetana K: Nucleoli in maturing blood cells. In Roath S (ed.): Topical Reviews in Haematology I. Wright, Bristol 1980, 115-137.

Smetana K: Structural features of nucleoli in blood, leukemic, lymphoma and myeloma cells. Eur J Histochem 46:125-132, 2002.

Smetana K, Busch H: Studies on silver stained nucleolar components. In Busch H, Crooke ST, Daskal Y (eds.), Effects of drugs on the cell nucleus. Academic Press, New York, 1979.

Smetana K, Cajthamlova H, Grebenova D, Hrkal Z: The 5-aminolaevulinic acid based photodynamic effects on nuclei and nucleoli of HL-60 leukemic granulocytic precursors. J Photochem Photobiol B Biol 59:80-99, 2000.

Smetana K, Chan PK, Jiraskova I, Busch H: Nucleolar coefficient and cytochemistry of human blood monocytes. Life Sci 60:2199-2205, 1997.

Smetana K, Jiraskova I, Cermak J: Incidence of nucleoli in erythroblasts of patients suffering from refractory anemia of myelodysplastic syndrome. Eur J Haematol 63:112-336, 1999.

Smetana K, Jiraskova I, Smetana K Jr., Cermak J: A short note on micronucleoli in the course of terminal maturation of human erythroblasts. Folia Biol (Praha) 47:14-17, 2001.

Smetana K, Jiraskova I, Perlaky L, Busch H: The silver reaction of nucleolar proteins in the main structural compartments of ring shaped nucleoli in smear preparations. Acta Histochem 101:167-183, 1999.

Smetana K, Jiraskova I, Sedlackova M, Dvorak R, Spatova M, Hozak P: Preferential silver reaction of nucleolar regions adjacent to fibrillar centers in ring shaped nucleoli of leukemic lymphocytes. Acta Histochem 100:257-270, 1998.

Stegh AH, Schickling O, Ehret A, Scaffidi C, Peterhansel C, Hofmann TG, Grummt I, Krammer PH, Peter ME: DEDD, a novel death effector domain-containing protein targeted to the nucleolus. EMBO J 17:5974-5986, 1998.

Wachtler F, Stahl E: The nucleolus: A structural and functional interpretation. Micron 24:473-505, 1993.

Weil M, Jacobson MD, Raff MC: Are caspases involved in the death of cells with a transcriptionally inactive nucleus? J Cell Sci 111:2707-2715, 1998.

Zatsepina OV, Chelidze PV, Chentsov YS: Changes in the volume and fibrillar centers with the inactivation of nucleoli at erythropoiesis. J Cell Sci 9:439-448, 1988.

Berger J, Machackova M, Berger Z: Effects of feed restriction on the nucleolar structure and function in lymphocytes. Basic Clin Pharmacol Toxicol 97:236-237, 2005.

Schulz A, Dhar S, Rylova S, Dbaibo G, Alroy J, Hagel C, Artacho I, Kohlschutter A, Lin S, Boustany RM: Impaired cell adhesion and apoptosis in a novel CLN9 Batten disease variant. Ann Neurol 56:342-350, 2004.

Smetana K, Chan PK, Marinov Y, Soucek J, Hrkal Z, Busch H: A short note on the nucleolar size and density in apoptotic leukemic granulocytic precursors (HL-60 cells). Life Sci 75:791-796, 2004.