ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 1 (2003), No 4, p 183-187

Glycophenotype of squamous epithelia: from laboratory to clinical practice

Karel Smetana Jr.

Address: Karel Smetana Jr., Charles University, 1st Faculty of Medicine, Institute of Anatomy, U nemocnice 3, 128 00 Praha 2, Czech Republic

Received 30th May 2003.
Published online 8th July 2003.

Full text article (pdf)

The epidermal stem cell plays a pivotal role in the function of squamous epithelia in physiological as well as in pathological conditions such as cancer. This review summarizes data about the glycobiology of normal squamous epithelia and related tumors with respect to epithelial cell differentiation and search for a glycophenotype specific for epidermal stem cells using labeled plant and endogenous lectins. Although the glycophenotype typical for epithelial cells at the stage of low differentiation level were found, no typical cell surface saccharidic markers of stem cells were detected. The nuclear binding of galectin-1 seems to be specific for the keratinocyte population prepared from hair follicles enriched for multipotent stem cells. The close topographical relationship of nuclear galectin-1 binding sites with SC35 splicing factor suggests some role for these glycoepitopes in pre-mRNA splicing. The data shown in this paper can be employed for diagnostic purposes and for cell therapy of skin defects and indicate the importance of the use of endogenous lectins as probes in biology and medicine.

epidermal stem cell; glycocode; endogenous lectin-galectin-carcinoma

Chiang C, Swan RZ, Gratchouk M, Bolinger M, Litingtung Y, Robertson EK, Cooper MK, Gaffield W, Westphal H, Beachy PA, Dlugosz AA: Essential role for sonic hedgehog during hair follicle morphogenesis. Develop Biol 205:1-9, 1999.

Dvorankova B, Motlik J, Holikova Z, Vacik J, Smetana K Jr.: Dolichos biflorus agglutinin-binding site expression in basal keratinocytes is associated with cell differentiation. Biol Cell 94:365-373, 2002.

Fan HR, Oro AE, Scott MP, Khavari PA: Induction of basal cell carcinoma features in transgenic human skin expressing Sonic Hedgehog. Nature Med 3:788-792, 1997.

Gabius H-J: Animal lectins. Eur J Biochem 243:543-576, 1997.

Gabius H-J, Andre S, Kaltner H, Siebert H-C: The sugar code: functional lectinomics. Biochim Biophys Acta 1572, 165-177, 2002.

Gandarillas A, Watt FM: c-Myc promotes differentiation of epidermal stem cells. Genes Dev 11 2869-2882, 1997.

Holikova Z, Hrdlickova-Cela E, Plzak J, Smetana K Jr., Betka J, Dvorankova B, Esner M, Wasano K, Andre S, Kaltner H, Hercogova J, Kodet R, Gabius H-J: Defining the glycophenotype of squamous epithelia by plant and mammalian lectins. Differentiationdependent expression of alpha2,6- and alpha2,3- linked N-acetylneuraminic acid in squamous epithelia and carcinomas and its differential effect on binding of the endogenous lectins galectin-1 and -3. APMIS 110:845-856, 2002.

Kanitakis J: Anatomy, histology and immunohistochemistry of normal human skin. Eur J Dermatol 12:390-401, 2002.

Labsky J, Dvorankova B, Smetana K Jr., Holikova Z, Broz L, Gabius H-J: Mannosides as crucial part of bioactive support for cultivation of human epidermal keratinocytes without feeder cells. Biomaterials 24:863-872, 2003.

Lavker RM, Sun T-T: Epidermal stem cells: Properties, markers, and location. Proc Natl Acad Sci USA 97:13473-13475, 2000.

Liang L, Bickenbach JR: Somatic epidermal stem cells can produce multiple cell lineages during development. Stem Cells 20, 21-33, 2002.

Pellegrini G, Dellambra E, Golisano O, Martinelli E, Fantozzi I, Bondanza S, Ponzin D, McKeon F, De Luca M: p63 identifies keratinocyte stem cells. Proc Natl Acad Sci USA 98:3156-3161, 2001.

Plzak J, Smetana K Jr., Betka J, Kodet R, Kaltner H, Gabius H-J: Endogenous lectins (galectin-1 and -3) as probes to detect differentiation-dependent alterations in human squamous cell carcinomas of the oropharynx and larynx. Int J Mol Med 5:369-372, 2000.

Plzak J, Holikova Z, Smetana K Jr., Dvorankova B, Hercogova J, Kaltner H, Motlik J, Gabius H-J: Differentiation-dependent glycosylation of cells in squamous cell epithelia detected by mammalian lectin. Cells Tissues Organs 171:135-144, 2002.

Plzak J, Smetana K Jr., Hrdlickova E, Kodet R, Holikova Z, Liu F-T, Dvorankova B, Kaltner H, Betka J, Gabius H-J: Expression of galectin-3-reactive ligands in squamous cancer and normal epithelial cells as a marker of differentiation. Int J Oncol 19, 59-64, 2001.

Trempus CS, Morris RJ, Bortner CD, Cotsarelis G, Faircloth RS, Reece JM, Tennant RW: Enrichment for living murine keratinocytes from the hair follicle bulge with cell surface marker CD34. J Invest Dermatol 120:501-511, 2003.

Watt FM: The stem cell compartment in human interfollicular epidermis. J Dermatol Sci 28:173-180, 2002.