Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 2 (2004), No 1, p 43-49




Acute toxicity and radioprotective effects of amifostine (WR-2721) or cystamine in single whole body fission neutrons irradiated rats

Pavel Kuna, Milan Dostal, Otakar Neruda, Josef Knajfl, Pavel Petyrek, Frantisek Podzimek, Jan Severa, Vaclav Svoboda, Jan Simsa, Stanislav Spelda, Jirina Vavrova, Jindriska Hermanska, Zdenek Prouza, Pavel Pitterman, Evzen Listik, Leos Navratil, Frantisek Spurny, Frantisek Konrad, Zdena Vilasova, Renata Havrankova

Address: Pavel Kuna, Department of Radiology and Toxicology, Faculty of Health and Social Studies, Matice skolske 17, 370 01 Ceske Budejovice, Czech Republic
pavel.kuna@tiscali.cz

Received 2nd August 2003.
Revised 6th October 2003.
Published online 30th October 2003.

Full text article (pdf)

SUMMARY
The radioprotective substances amifostine (WR-2721) and cystamine were tested in rats following their parenteral administration (i.p., i.m., and i.v.). Cystamine is more toxic than amifostine in mice as well as in rats. Amifostine was less toxic after intravenous injection. The radioprotective effects of WR-2721 (160 mg.kg-1) and cystamine (40 mg.kg-1) were not significant when they were administered parenterally 15-20 mins before lethal doses of whole body fission neutron irradiation in the thermal column of reactor VVR-S and 30-days lethality served as an integral criterion of postradiation injury to the rat body. The fission neutrons spectrum was characterized by mean energy 0.9-1.0 MeV with 30-40% fluency participation of moderate (E=0.1 MeV) neutrons. The contamination with gamma rays was 22-30%; the dose rate of whole irradiation was within 0.3 to 0.8 Gy.min-1.

KEY WORDS
amifostine; WR-2721; cystamine; radioprotective effect; fission neutrons irradiation


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