ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 3 (2005), No 1, p 37-46

Biochemical and haematological differentiation of opiate addicts from healthy subjects. A case control study

Jose Fernando Diaz-Flores Estevez, Felicitas Diaz-Flores Estevez, Carlos Hernandez Calzadilla, Elena Maria Rodriguez Rodriguez, Carlos Diaz Romero, Lluis Serra-Majem

Address: Carlos Diaz Romero, Laboratory of Clinical Analysis of the Drug Rehabilitation Center, Youth Association Cooperation "San Miguel", 38006-Santa Cruz de Tenerife, Spain

Received 12th January 2005.
Revised 21st January 2005.
Published online 24th January 2005.

Full text article (pdf)

The biochemical and haematological parameters of nutritional interest were determined in the serum of opiate addicts in order to compare them with those obtained in healthy subjects. The blood of 106 opiate addicts in detoxification treatment (n=19) or in Methadone Maintenance Treatment Program (MMTP) (n=87) was studied. Opiate addicts presented lower levels in the number of red cells, cholesterol, albumin, retinol, alpha-tocopherol, folic acid, K and Se and higher levels in the number of leukocytes, GOT, GPT and Na, than the control group. The opiate addicts in MMTP had higher levels of glucose, triglycerides, Mg and P than the opiate addicts in the detoxification treatment and control groups. Significant correlations between the three vitamins (folic acid, retinal and alpha-tocopherol) were observed and the graphic representations suggest a biochemical differentiation between opiate addicts and healthy subjects. Factor analysis made it possible to select seven factors explaining 66.2% of the total variance, and representing the first and fourth factor, opiate addicts tended to separate from the control group.

opiate addicts; correlation and factor analysis; detoxification; methadone

Christakis G, Stimmel B, Rabin J: Nutritional Status of heroin users enrolled in methadone maintenance. Proc Natl Conf Methadone Treatm 1:494-500, 1973.

Diaz-Flores Estevez JF, Diaz-Flores Estevez F, Hernandez Calzadilla C et al.: Application of linear discriminant analysis to the biochemical and haematological differentiation of opiate addicts from healthy subjects. A case control study. Eur J Clin Nutr 58:449-455, 2003.

Farrell M, Ward J, Mattick R et al.: Methadone maintenance treatment in opiate dependence. Br Med J 309:997-1001, 1994.

Gambera SE, Clarke JAK: Comment on dietary intake of drug-dependent persons. J Am Diet Assoc 68:155-157, 1976.

Grant A, DeHoog S: Nutritional Assessment and Support, 3rd edn. Grant-DeHoog, Seattle, 1985.

Henriquez Sanchez P, Diaz Romero C, Rodriguez Rodriguez E et al.: Evaluacion bioquimica del estado nutricional de la poblacion Canaria. Arch Latinoamer Nutr 50:43-54, 2000.

Kabrtt J, Wilczek H, Svobodova J, Haluzic M, Kotrlikova E: Nutritional status of drug addicts in a methadone program. National Library of Medicine 138:693-695, 1999.

Morabia A, Fabre J, Chee E et al.: Diet and opiate addiction: a quantitative assessment of the diet of non-institutionalizated opiate addicts. Br J Addict 84:173-180, 1989.

Rodriguez-Delgado M., Diaz-Flores Estevez JF, Diaz-Flores Estevez F, Hernandez Calzadilla C, Diaz Romero C: Fast determination of retinol and alpha-tocopherol in plasma by LC. J Pharm Biomed Anal 28:991-997, 2002.

Santolaria-Fernandez FJ, Gomez-Sirvent JL, Gonzalez-Reimers CE et al.: Nutritional assessment of drug addicts. Drug Alcohol Depend 38:11-18, 1995.

Steghens JP, van Kappel AL, Riboli E, Collombel C: Simultaneous measurement of seven carotenoids, retinol and alpha-tocopherol in serum by high-performance liquid chromatography. J Chromatogr B694:71-81, 1997.

Verde Mendez C, Diaz-Flores JF, Sanudo RI, Rodriguez Rodriguez E, Diaz Romero C: Habitos alimentarios en opiaceodependientes incluidos en un Programa de Mantenimiento con Metadona. Alim Nutr Salud 9:61-68, 2002.