Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 3 (2005), No 3, p 123-128




Nycthemeral change of some haematological parameters in horses

Giuseppe Piccione, Francesco Fazio, Elisabetta Giudice1, Fortunata Grasso, Massimo Morgante

Address: Giuseppe Piccione, Dipartimento di Morfologia, Biochimica, Fisiologia e Produzioni animali, Facolta di Medicina Veterinaria, Sezione di Fisiologia Veterinaria, Universita degli Studi di Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy
Giuseppe.Piccione@unime.it

Received 28th May 2005.
Revised 9th June 2005.
Published online 29th June 2005.

Full text article (pdf)

SUMMARY
Many physiological processes of domestic animals exhibit daily rhythmicity. The goal of the present study was to investigate, in horses, the existence of the daily rhythms of some haematological parameters: red blood cells (RBC), white blood cells (WBC), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and platelets (PLT). Blood samples from 6 Thoroughbred mares were collected at 4-hour intervals for 48 hours (starting at 08:00 hours on day 1 and finishing at 04:00 on day 2) via an intravenous cannula inserted into the jugular vein. ANOVA showed a highly significant effect of time on haemoglobin and on haematocrit, in all the horses, on either day, with p values < 0.05. Cosinor analysis identified the periodic parameters and their acrophases (expressed in hours) during the 2 days of monitoring. RBC, Hb, Hct and MCV showed nycthemeral variations included between 17.44 and 22.28, which could suggest the hypothetical influence of various exogenous factors.

KEY WORDS
circadian rhythm; haematological parameters; horse


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CITED

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