Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 4 (2006), No 4, p 197-203




Cytogenetic methods and biomonitoring of occupational exposure to genotoxic factors

Tomas Adamus, Irena Mikulenkova, Lubomir Dobias, Jaroslava Havrankova, Tomas Pek

Address: Tomas Adamus, Faculty of Health and Social Studies, University of Ostrava, Department of Medical Examination Methods and Medical Biology, Syllabova 19, 703 00 Ostrava 3, Czech Republic
tomas.adamus@post.cz

Received 20th June 2006.
Revised 11th September 2006.
Published online 27th October 2006.

Full text article (pdf)
Full text article (html)

SUMMARY
The professional aim of this project was to map the detection of genotoxicants using cytogenetic methods. It summarizes the results of four studies carried out at three different workplaces in different industries. The main aim of the study was also to monitor types of exposure to genotoxicants and their impact on the human population: The exposure of blast furnace workers in the steel industry to genotoxic factors; the exposure of coke oven plant workers to genotoxic factors, and the exposure to genotoxic factors in workplaces in the chemical industry - in particular to heavy metals (Ni, Cd). Cytogenetic analysis and the micronucleus test are sensitive methods in the field of biomonitoring of human exposure in industry, and play an important role in the prevention of damage to human health.

KEY WORDS
genotoxicants; micronucleus test; binucleated cells; furnace workers


REFERENCES
Adamus T, Mikulenkova I, Kusova J et al.: Entry about using cytogenetic research methods for monitoring of impact genotoxic working environment factors (in Czech). In Conference proceedings No. 27 - Czech and Slovak Company for Environmental Mutagenesis Working Days. NCO NZO Brno, May 5-7, 2004, pp. 35-36.

AHEM Supplement 20/1989: Methods for biological monitoring of genotoxic effects of environmental factors (in Czech). SZU, Praha 1989.

AHEM 3/2003: Regular operation standards for biological monitoring of environmental genotoxic impacts (in Czech). SZU, Praha 2003.

Albertini RJ, Niclas JA, O´Neill JP: Future research directions for evaluating human genetic and cancer risk from environmental exposures. Environ Health Perspect 104 (Suppl. 3):503-510, 1996.

Albertini RJ, Anderson D, Douglas GR et al.: IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety. Mutat Res 463:111-172, 2000.

Brusick DJ: Methods for Genetic Risk Assessment. Lewis Publishers, CRC Press, Boca Raton, Florida 1994.

Buchancov J, Klimentova G, Sulcova M et al.: Pracovne lekarstvo a toxikologia. Osveta, Martin 2003.

Cerna M, Rossner P: Using of short - term methods for monitoring of genotoxic working environment factors impacts (in Czech). Cs. Hygiena 33:105-109, 1988.

Cikrt M, Kneidlova M, Tucek M et al.: Working environmental and working procedure factors - their affections and effects to humans - chemical factors (in Czech). In Provaznik K, Komarek L, Cikrt M (eds.): Manual prevence v lekarske praxi I.-V. dil, Souborne vydani, V., 2.2:517-540, 1998.

Daisey JM, Kneip TJ, Hawryluk I, Mukai F.: Seasonal variations in the bacterial mutagenicity of airborne particulate organic matter in New York City. Environ Sci Technol 4/12:1478-1490, 1980.

Dipple A, Moschel RC, Digger CAH: Polynuclear aromatic carcinogens. In Searle CE (ed.): Chemical Carcinogens. ACS Monograph No. 173, American Chemical Society, Washington D.C. 1976, pp. 324-365.

Dobias L, Vit M: Chemical carcinogens and occupational environment (in Czech). In Cikrt M, Malek B (eds.): Pracovni lekarstvi I. dil - Hygiena prace, CIVOP, Praha 1995, pp. 153-163.

Dobias L, Vit M, Malachova K, Havrankova J: Occupational exposure to heavy metals in industry. In Proceedings of the 2nd International Conference on Trace Elements Effects on Organisms and Environment, Katowice, Poland 1998, pp. 5-10.

Dobias L, Vit M: Chemical carcinogens (in Czech). In Jirak Z (ed.): Hygiena prace v zakladnich vyrobnich odvetvich II, IDVZP, Brno 1995.

Fenech M, Bonassi S, Turner J et al.: Intra - and inter - laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Result of international slide - scoring excercise by the HUMN project. Mutat Res 534:45-64, 2003.

Fenech M, Holland N, Chang PW et al.: The HUman MicroNucleus Project-An international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans. Mutat Res 428:271-283, 1999.

Fenech M, Morley A: Measurement of micronuclei in lymphocytes. Mutat Res 147:29-36, 1985.

Hungerfod DA: Leucocytes cultured from small inocula of whole blood and preparation of metaphase chromosomes by treatment with hypotonic KCl. Stain Technol 40:333-338, 1965.

Hunter WJ, Aresini G, Haigh R, Papadopoulos P: Occupational exposure limits for chemicals in the European Union. Occup Environ Med 54:217-222, 1997.

IAEA: Cytogenetic Analysis for Radiation Dose Assessment. Technical Report Series No. 405, A Manual, International Atomic Energy Agency, Vienna 2001.

IARC: Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. Vol. 34, IARC, Lyon, France 1984.

Kusova J, Dobias L, Adamcik M et al.: Metallurgical technologies and genotoxic risk. Human Monitoring for Genetic Effects, NATO Science Series I: Life and Behavioural Sciences, Vol. 351, IOS Press, Amsterdam 2003, pp. 125-131.

Kusova J, Havrankova J, Dobias L et al.: Multivitamin prophylaxis and exposure to carcinogenic polycyclic aromatic hydrocarbons of coke oven plant workers. In Workshop Assessment of occupational and environmental exposure to genotoxic substances - Methodological approach, Ustron, Poland, Oct. 4-7, 2000, pp. 47-48.

Lewtas J: Experimental evidence for the carcinogenicity of air pollutans. In Tomalis L (ed.): Air Pollution and Human Cancer, Springer - Verlag, Berlin 1990, pp. 49-61.

Rossner P, Bavorova H, Ocadlikova D et al.: Chromosomal aberrations in peripheral lymphocytes of children as biomarkers of environmental exposure and life style. Toxicol Lett 134:79-85, 2002.

Rossner P: Methods of biological genotoxic effects factors monitoring in the occupational environment - Cytogenetic analysis of peripheral lymphocytes (in Czech). Ceske pracovni lekarstvi (Suppl. 1):34-39, 2000.

Smerhovsky Z, Landa K, Rossner P et al.: Risk of cancer in an occupationally exposed cohort with increased level of chromosomal aberrations. Environ Health Perspect 109:41-45, 2001.

Zudova Z, Samkova I.: Usti nad Labem area pregnancy results analysis (in Czech). AHEM 5:7-9, 1980.
CITED

0

BACK