ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 5 (2007), No 1, p 19-23

Cell migration and the ganglioside composition of the cell lines derived from Reuber hepatoma

Lenka Dudkova, Lucie Muchova, Eva Brozova, Frantisek Smid

Address: Institute of Clinical Biochemistry and Laboratory Diagnostics, General University Hospital and First Faculty of Medicine Charles University in Prague, U nemocnice 2, Prague 2, 120 00, Czech Republic

Received 23rd May 2006.
Revised 16th October 2006.
Published online 4th December 2007.

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The migration potency of differentiated (H-4-II-E, H-4-II-E-C3) and dedifferentiated (H-5) cell lines originated from Reuber hepatoma H 35, and their connection to the morphology and expression of gangliosides were examined. The migration capacity tested by a Transwell assay was fifteen times higher in H-5 cells compared to H-4-II-E cells. The ganglioside pattern was assessed by thin-layer chromatography (TLC). H-4-II-E and H-4-II-E-C3 cell lines expressed Fuc-GM1, which was not found in H-5 cells, whereas H-5 expressed GM3, which was absent in differentiated cell lines. GM3 ganglioside is thought to be one of the key molecules involved in signal transduction of mammalian cells. We conclude that changes in the migration capacity of various hepatoma cell lines might relate to their ganglioside spectra.

hepatoma; cell migration; ganglioside; GM3

Bohata J, Smid F, Krenova D, Kawashima I, Tai T, Nagai Y: Immunochromatographical analysis of gangliosides from rat liver and Nnitrosomorpholine induced hepatocellular carcinoma. Int J Bio-Chromatogr 4:43-51, 1998.

Deschatrette J, Weiss MC: Characterization of differentiated and dedifferentiated clones from a rat hepatoma. Biochimie 56:1603-1611, 1974.

Facci L, Skaper SD, Presti D, Kirschner G, Leon A, Chiecobianchi L: Exogenously administered gangliosides fail to increase in vivo metastatic frequency or in vitro growth of murine neoplastic cells. Clin Exp Metastasis 8:181-192, 1990.

Garcia-Pelayo MC, Garcia-Peregrin E, Martinez- Cayuela M: Differential translational effects of myristic acid and eicosapentaenoic acid on 3- hydroxy-3-methylglutaryl-CoA reductase from Reuber H35 hepatoma cells. Exp Biol Med 229:781-786, 2004.

Hashiramoto A, Mizukami H, Yamashita T: Ganglioside GM3 promotes cell migration by regulating MAPK and - Fos/AP. Oncogene 25:3948-3955, 2006.

Hakomori S, Igarashi Y: Functional role of glycosphingolipids in cell recognition and signalling. J Biochem 118:1091-1103, 1995.

Hartree EF: Determination of protein: A modification of the Lowry method that gives a linear photometric response. Anal Biochem 48:122-127, 1972.

Hyuga S, Yamagata S, Takatsu Y, Hyuga M, Nakanishi H, Furukawa K, Yamagata T: Suppression by ganglioside GD1a of migration capability, adhesion to vitronectin and metastatic potential of highly metastatic FBJLL cells. Int J Cancer 83:685-691, 1999.

Kawamura S, Ohyama C, Watanabe R, Satoh M, Saito S, Hoshi S, Gasa S, Orikasa S: Glycolipid composition in bladder tumor: A crucial role of GM3 ganglioside in tumor invasion. Int J Cancer 94:343-347, 2001.

Ladish S, Gillard G: A solvent partition method for microscale ganglioside purification. Anal Biochem 146:220-231, 1985.

Lang Z, Guerrera M, Li R, Ladisch S: Ganglioside GD1a enhances VEGF-induced endothelial cell proliferation and migration. Biochem Biophys Res Commun 282:1031-1037, 2001.

McKallip R, Ruixiang L, Ladisch S: Tumor gangliosides inhibit the tumor-specific immune response. J Immunol 163:3718-3726, 1999.

Mitsuzuka K, Handa K, Satoh M, Arai Y, Hakomori S: A specific microdomain ("glycosynapse 3") controls phenotypic conversion and reversion of bladder cancer cells through GM3-mediated interaction of alpha3beta1 integrin with CD9. J Biol Chem 280:35545-35553, 2005.

Ono M, Handa K, Sonnino S, Withers DA, Nagai H, Hakomori S: GM3 ganglioside inhibits CD9-Facilitated haptotactic cell motility: Coexpression of GM3 and CD9 is essential in the downregulation of tumor cell motility and malignancy. Biochemistry 40:6414-6421, 2001.

Pitot HC, Peraino C, Morse PA, Potter VR: Hepatomas in tissue culture compared with adapting liver in vivo. Natl Cancer Inst Monogr 13:229-245, 1964.

Reuber MD: A Transplantable bile-secreting hepatocellular carcinoma in the rat. J. Natl. Cancer Inst. 26:891-897, 1961.

Saha S, Mohanty KC, Mallick P: Gangliosides enhance migration of mouse B16-melanoma cells through artificial basement membrane alone or in presence of laminin or fibronectin. Indian J Exp Biol 43:1130-1138, 2005.

Shurin GV, Shurin MR, Bykovskaia S, Shogan J, Lotze MT, Barksdale EM: Neuroblastomaderived gangliosides inhibit dendritic cell generation and function. Cancer Res 60:363-369, 2001.

Smid F, Reinisova J: A densitometric method for the determination of gangliosied after their separation by thin-layer chromatography and detection with resorcinol reagent. J Chromatogr 86:200-204, 1973.

Wang XQ, Sun P, Paller AS: Ganglioside modulation regulates epithelial cell adhesion and spreading via ganglioside-specific effects on signalling. J Biol Chem 277:40410-40419, 2002.

Wiebel FJ, Park SS, Kiefer F, Gelboin HV: Expression of cytochromes P-450 in rat hepatoma cells. Analysis by monoclonal antibodies specific for cytochromes P-450 from rat liver induced by 3-methylcholanthrene or phenobarbital. Eur J Biochem 145:455-462, 1984.

Wu G, Ledeen R: Quantification of gangliotetraose gangliosides with cholera toxin. Anal Biochem 173:220-231, 1985.

Zvibel I, Fiorino AS, Brill S, Reid LM: Phenotypic characterization of rat hepatoma cell lines and lineage-specific regulation of gene expression by differentiation agents. Differentiation 63:215-223, 1998.