Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 5 (2007), No 1, p 19-23




Cell migration and the ganglioside composition of the cell lines derived from Reuber hepatoma

Lenka Dudkova, Lucie Muchova, Eva Brozova, Frantisek Smid

Address: Institute of Clinical Biochemistry and Laboratory Diagnostics, General University Hospital and First Faculty of Medicine Charles University in Prague, U nemocnice 2, Prague 2, 120 00, Czech Republic
lenka.dudkova@centrum.cz

Received 23rd May 2006.
Revised 16th October 2006.
Published online 4th December 2007.

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SUMMARY
The migration potency of differentiated (H-4-II-E, H-4-II-E-C3) and dedifferentiated (H-5) cell lines originated from Reuber hepatoma H 35, and their connection to the morphology and expression of gangliosides were examined. The migration capacity tested by a Transwell assay was fifteen times higher in H-5 cells compared to H-4-II-E cells. The ganglioside pattern was assessed by thin-layer chromatography (TLC). H-4-II-E and H-4-II-E-C3 cell lines expressed Fuc-GM1, which was not found in H-5 cells, whereas H-5 expressed GM3, which was absent in differentiated cell lines. GM3 ganglioside is thought to be one of the key molecules involved in signal transduction of mammalian cells. We conclude that changes in the migration capacity of various hepatoma cell lines might relate to their ganglioside spectra.

KEY WORDS
hepatoma; cell migration; ganglioside; GM3


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