Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 5 (2007), No 1, p 39-47




Maldi tof mass spectrometry of selected mycotoxins in barley

Shaban Elosta, Dagmar Gajdosova, Blanka Hegrova, Josef Havel

Address: Josef Havel, Department of Analytical Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic
havel@chemi.muni.cz

Received 16th November 2006.
Published online 10th January 2007.

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SUMMARY
A novel method for the simultaneous determination of the trichothecene mycotoxins deoxynivalenol (DON), nivalenol (NIV), 3-acetyl-deoxynivalenol (3-ADON), and 15-acetyl-deoxynivalenol (15-ADON) in barley and malt extracts has been developed using Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI TOF MS). This technique enables highly sensitive and fast analysis and/or detection using very small samples. In this work several matrices were examined and the most suitable ones were identified. Statistical analysis was carried out to verify the ability of the system to determine the mycotoxins in a real sample. Test for the accuracy of the method, repeatability, limit of detection (LOD) and limit of quantification (LOQ) were studied. Moreover, the accuracy of the method was confirmed by comparing analytical data to certified values from reference materials for those mycotoxins. In addition, the comparison of analytical parameters for the determination of DON, 3-ADON, 15-ADON, and Nivalenol was performed. This work opens the possibility of very sensitive determination of the selected mycotoxins in barley, malt, cereals and food.

KEY WORDS
malt; deoxynivalenol; nivalenol; 3-acetyl-deoxynivalenol; 15-acetyl-deoxynivalenol; MALDI TOF MS


REFERENCES
Berger U, Oehme M, Kuhn F: Quantitative determination and structure elucidation of type A- and B-trichothecenes by HPLC/Ion Trap Multiple Mass Spectrometry. J Agric Food Chem 47:4240-4245, 1999.

Blechova P, Havlova P, Gajdosova D, Havel J: New possibilities of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry to analyze barley malt quality. Highly sensitive detection of mycotoxins. Environ Toxicol 4:403-408, 2006.

Cole RJ, Cox RH: Handbook of Toxic Fungal Metabolites. Academic Press, New York 1981, pp. 152-263.

Eckschlager K, Horsak I, Kodejs Z: Vyhodnocovani analytickych vysledku a metod. SNTL, Praha 1980.

Francisco M, Valle A, Angel M, Jose VG-A, Amparo L, Misericordia J, Rufino M: Comparative assessment of solid-phase extraction clean-up procedures, GC columns and perfluoroacylation reagents for determination of type B trichothecenes in wheat by GC-ECD. Talanta 6:194-201, 2005.

Fuchs E, Rabus B, Handl J, Binder EM: Type Atrichothecenes - quantitative analysis using LC-MS and occurrence in Austrian maize and oats. Mycotoxin Res 19:56, 2003.

Olson J, Borjesson T, Lundstedt T, Schnurer J: Detection and quantification of ochratoxin A and deoxynivalenol in barley grains by GCMS and electronic noise. Int. J. Food Microbiol. 72:203-214, 2002.

Onji Y, Aoki Y, Tani N, Umebayashi K, Kitada Y, Dohi Y: Direct analysis of several Fusarium mycotoxins in cereals by capillary gas chromatography-mass spectrometry. J Chromatogr A 815:59-65, 1998.

Prelusky DB, Rotter BA, Rotter RG: In Miller JD, Trenholm HL (eds.): Mycotoxins in Grain, Compounds Other than Aflatoxins. Eagan Press, St. Paul, Minn 1994, p. 359.

Radová Z, Holadova K, Hajslova J: Comparison of two clean-up principles for determination of trichothecenes in grain extract. J Chromatogr A 829:259-267, 1998.

Razzazi-Fazele E, Bohm J, Luf W: Determination of nivalenol and deoxynivalenol in wheat using liquid chromatography-mass spectrometry with negative ion atmospheric pressure chemical ionisation. J Chromatogr A 854:45-55, 1999.

Razzazi-Fazeli E, Rabus B, Cecon B, Bohm J: Simultaneous quantification of Atrichothecene mycotoxins in grains using liquid chromatography-atmospheric pressure chemical ionisation mass spectrometry. J Chromatogr A 968:129-142, 2002.

Sweeney MJ, Dobson ADW: Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int J Food Microbiol 43:141-158, 1998.

Tanaka K, Waki H, Ido Y, Akita S, Yoshida Y, Yoshida T: Protein and polymer analyses up to m/z 100,000 by Laser Ionization Time-of- Flight Mass Spectrometry. Rapid Commun Mass Spectrom 8:445-448, 1988.

Tanaka T, Yoneda A, Inoue S, Sugiura Y, Ueno Y: Simultaneous determination of trichothecene mycotoxins and zearalenone in cereals by gas chromatography-mass spectrometry. J Chromatogr A 882:23-28, 2000.

Trucksess MW, Ready DE, Pender MK, Ligmond CA, Wood GE, Page SW: Determination and survey of deoxynivalenol in white flour, whole wheat flour, and bran. J AOAC Int 79:883-887, 1996.

Trucksess MW, Thomas F, Young K, Stack ME, Fulgueras WJ, Page SW: Survey of deoxynivalenol in U. S. 1993 wheat and barley crops by enzyme-linked immunosorbent assay. J AOAC Int 78:631-636, 1995.

Ueno Y: In Draper HH (ed.): Nutritional Research. Plenum Press, New York 1983, p. 225.

Van Egmond HP, Jonker MA: Worldwide regulations for mycotoxins in food and feed, Draft FAO Food and Nutrition Paper, 2004.
CITED

Vujanovic V, Chau HW. Monitoring Fusarium complex mycelia replacement by mycopathogenic Sphaerodes using alcohol percentage test, qRT-PCR and HPLC. Physiol Molecul Plant Pathol. 80: 28-34, 2012.

Kong X, Cheng P. Application of Nanodiamonds in Biomolecular Mass Spectrometry. Mater. 3: 1845-1862, 2010.

Bhat R, Rai RV, Karim AA: Mycotoxins in Food and Feed: Present Status and Future Concerns. Compre Rev Food Sci Food Safety 9:57-81, 2010.

Maragos CM, Busman M: Rapid and advanced tools for mycotoxin analysis: a review. Food Addit Contam Part A-Chem Anal Control Exp Risk Assessment 27:688-700, 2010.

Chau HW, Goh YK, Si BC, Vujanovic V: Assessment of alcohol percentage test for fungal surface hydrophobicity measurement. Lett Appl Microbiol 50:295-300, 2010.

Liu Y, Delwiche S, Dong, Y: Feasibility of FT-Raman spectroscopy for rapid screening for DON toxin in ground wheat and barley. Food Additt Contam - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment 26:1396-1401, 2009.

Houska J, Panyala NR, Pena-Mendez EM, Havel J: Mass spectrometry of nanodiamonds. Rapid Commun Mass Spectrom 23:1125-1131, 2009.

Rahmani A, Jina S, Soleimany F: Qualitative and quantitative analysis of mycotoxins. Compre Rev Food Sci and Food Safety 8:202-251, 2009.

Tejral G, Panyala NR, Havel J: Carbon nanotubes: Toxicological impact on human health and environment. J Appl Biomed 7:1-13, 2009.

Jedlickova L, Gadas D, Havlova P, Havel J: Determination of ergosterol levels in barley and malt varieties in the Czech Republic via HPLC. J Agric Food Chem 56:4092-4095, 2008.


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