ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 5 (2007), No 2, p 77-80

Lactate dehydrogenase isoenzyme pattern in the liver tissue of chemically-injured rats treated by combinations of diphenyl dimethyl bicarboxylate

Laila Faddah, Nabil Abdel-Hamid, Yaser Abul-Naga, Sanaa Ibrahim, Ahmed Mahmoud

Address: Nabil Mohie Abdel-Hamid, Department of Biochemistry, Faculty of Pharmacy, El-Minia University, El Minia, Egypt

Received 15th October 2006.
Revised 22nd December 2006.
Published online 12th March 2007.

Full text article (pdf)
Full text article (html)

The purpose of our study is to evaluate the protective effect of diphenyl dimethyl bicarboxylate (DDB) in combination with some antioxidants, namely vitamin C (V.C), vitamin E (V.E), and selenium (Se), in liver damage induced by carbon tetrachloride (0.2 ml/kg body weight). This was done by monitoring the liver total and fractional lactate dehydrogenase (LDH) activities. The results revealed a significant increase in the activity of liver total LDH activity in CCl4 - intoxicated rats with a significant increase in both LDH3 and 4 and a significant decrease in LDH5. LDH2 disappeared after CCl4 treatment and neither DDB nor its combinations could restore this permanent change. DDB alone significantly decreased the CCl4-raised total LDH and LDH4, but still far from the control and failed to correct LDH3 and 5 variations. A combination of DDB and V.C, V.E and Se showed the best corrective potential in both total LDH and LDH3 activities, without correcting the increased LDH4, nor the decreased LDH5 isoenzyme. Although this combination was previously reported to correct liver function disturbances, it seems that CCl4 and consequently hepatitis C may induce some irreversible, non-curable changes by DDB or even by additional antioxidants. Its clinical usefulness seems to be through different metabolic pathways, not including correction of LDH disturbances, which necessitates additional investigation for other adjunct medicines for treating liver fibrosis in clinical practice.

LDH isoenzymes; liver fibrosis; DDB; CCI4; rats

Babson AL, Babson SR: Kinetic colorimetric measurement of serum lactate dehydrogenase activity. Clin Chem 19:766-769, 1973.

Berne D, Kyriakopoulos A, Sceid S, Gressner H: Effects of chemical form and dosage on the incorporation of selenium into tissue protein in rats. J Nutr 121:806, 1991.

Dietz AA, Lubrano T: Separation and quantitation of lactate dehydrogenase isoenzymes by disc electrophoresis. Anal Biochem 20:246-257, 1967.

Duncan DB: Multiple range and multiple F tests. Biometrics 11:1-42, 1955.

El-Sawy SA, El-Shafey AM, El-Bahrawy HA: Effect of dimethyl diphenyl bicarboxylate on normal and chemically-injured liver. East Mediterr Health J 8:95-104, 2002.

Fu T, Liu G: Protective effects dimethyl-4, 4- dimethoxy-5, 6, 5, 6-dimethylenedioxybiphenyl -2, 2-dicarboxylate on damages of isolated rat hepatocytes induced by carbon tetrachloride and D-galactosamine. Biomed Environ Sci 5:185-194, 1992.

Gao M, Zhang J, Liu G: Effect of diphenyl dimethyl bicarboxylate on concanavalin A - induced liver injury in mice. Liver Int 25:904-912, 2005.

Henderson AR: Isoenzymes of lactate dehydrogenase. In Bergmeyer HU (ed): Methods of Enzymatic Analysis. Vol. III (Third Edition), VCH, USA and Canada 1986, pp. 138.

Ip SP, Ko KM: The crucial antioxidant action of schisandrin B in protecting against carbon tetrachloride hepatotoxicity in mice. A comparative study with butylated hydroxyl toluene. Biochem Pharmacol 52:1687-93, 1996.

Kim SN, Kim SY, Yim HK, Lee WY, Han KS, Kim SK, Yoon NY, Kim YC: Effect of dimethyl-4, 4-dimethoxy 5,6,5-,6-dimethylenedioxybiphenyl- 2, 2-dicarboxylate (DDB) on chemical-induced liver injury. Biol Pharm Bull 22:93-5, 1999.

Kory M, Susan C: Clinical Chemistry: Concepts and Application. W.B. Saunders Company, Harcourt Brace Jovanovich, Inc., Philadelphia, 1993, pp. 238.

Liang B, Lane L, Watson R: Vitamin E stimulation of disease and immune function. J Antioxid 4:201, 1995.

Palmer AM, Thomas CR, Gopaul N, Dhir S, Anggard EE, Poston L, Tribe RM: Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocindiabetic rat. Diabetologia 41:148-56, 1998.

Qing WG, Liu GT: Protective effect of dimethyl, 4, 4, dimethoxy 5, 6, 5, 6-dimiethylene dioxybiphenyl-2, 2-dicaboxylate (DDB) against carcinogen-induced rat liver nuclear DNA damage. Biomed Environ Sci 5:201-208, 1992.

Rodrigue F, Boyer O, Feillet F, Lemonnier A: Lactate dehydrogenase LD5 : LD2 ratio as an indicator of early graft function and complications following pediatric orthotopic liver transplantation. Transplant Proc 27:1871-1874, 1995.

Salplachta J, Necas J: Lactate Dehydrogenase isoenzymes pattern in tissues and serum of the calf. Acta Vet Brno 69:267-275, 2000.

Yasuda J, Syuto B, Too K, Ohfuji S: Lactate dehydrogenase isoenzyme pattern in bovine liver tissue. Nippon Juigaku Zasshi 51:733-739, 1989.

Zhu M, Lin KF; Yeung RY, Li RC: Evaluation of the protective effects of Schisandra chinensis of phase I drug metabolism using CCl4 intoxication model. J Ethnopharmacol 67:61-68, 1999.

Haque MM, Im H-Y, Seo J-E, Hasan M, Sarma S, Woo K, Kwon O-S. Evaluation of CdSe/CdS-PEG-FA quantum dots: Distribution and observable-adverse-effect-level in mice after intravenous injection H. J Pharm Invest. 42: 203-212, 2012.

Hallegue D, Tebourbi O, Kacem K, Sakly M, Ben Rhouma K. Impact of dieldrin on liver morphological and biochemical parameters of rats. Toxicol Industrial Health. 26: 131-137, 2010.