ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 5 (2007), No 2, p 105-113

Urtica dioica extract reduces platelet hyperaggregability in type 2 diabetes mellitus by inhibition of oxidant production, Ca2+ mobilization and protein tyrosine phosphorylation

Mohammed El Haouari, Isaac Jardin, Hasane Mekhfi, Juan A. Rosado, Gines M. Salido

Address: J. A. Rosado, Department of Physiology, University of Extremadura, Av. Universidad s/n. Caceres 10071, Spain

Received 21st March 2007.
Revised 23rd April 2007.
Published online 7th May 2007.

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Platelet hyperaggregability is involved in the pathogenesis of type 2 diabetes mellitus. Thrombin-evoked platelet aggregation includes the activation of several intracellular pathways, including endogenous generation of reactive oxygen species (ROS), Ca2+ mobilization and protein tyrosine phosphorylation. Here we show that crude aqueous extract from Urtica dioica reduces thrombin-evoked aggregation in platelets from healthy donors and diabetics, in a concentration-dependent manner. U. dioica extract showed a potent antioxidant activity and prevented thrombin-evoked ROS generation in platelets from healthy and diabetic donors. Treatment with U. dioica extract reduced Ca2+ entry induced by thrombin or selective depletion of the two Ca2+ stores in platelets (without altering Ca2+ release), reduced protein tyrosine phosphorylation and reversed the abnormal Ca2+ mobilization and tyrosine phosphorylation in platelets from diabetics. We conclude that extract from U. dioica shows antiaggregant actions and might be used for the treatment and/or prevention of cardiovascular complications associated with type 2 diabetes mellitus.

platelets; type 2 diabetes mellitus; Urtica dioica; calcium mobilization; antioxidant; tyrosine phosphorylation

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