Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 5 (2007), No 2, p 81-84




Assay of radical scavenging activity of antidotes against chemical warfare agents by DPPH test using sequential injection technique

Vit Koleckar, Daniel Jun, Lubos Opletal, Ludek Jahodar, Kamil Kuca

Address: Kamil Kuca, Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01, Hradec Kralove, Czech Republic
kucakam@pmfhk.cz

Received 2nd November 2006.
Revised 3rd January 2007.
Published online 19th March 2007.

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SUMMARY
Free radicals are believed to play an important role in many pathological states. Consequently antioxidants are receiving increased attention in medicinal research. As part of studies of the biological effects of the antidotes against chemical warfare agents currently used in the Czech Armed Forces, eleven compounds were assayed for their free radical scavenging activity. An optimized PC-controlled sequential injection analysis (SIA) system with spectrophotometric detection was used for evaluation of the antioxidation activity of the antidotes. The automated method is based on the known reaction of 2,2´- diphenyl-1-picrylhydrazyl radical (DPPH) with antioxidants. Only the radical scavenging activity of the tested compound 2,3-dimercaptopropanol (BAL) was found to be weaker compared to the reference antioxidant acidum ascorbicum. Other tested antidotes did not possess any DPPH radical scavenging activity. The results obtained show that a correlation between the required biological activity (prophylaxis or treatment of intoxications by warfare agents) and possible antioxidation activity of the tested antidotes is doubtful.

KEY WORDS
antioxidant; radical; analysis; antidote; warfare agent


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