Free radicals are believed to play an important role in many pathological states. Consequently antioxidants are receiving increased attention in medicinal research. As part of studies of the biological effects of the antidotes against chemical warfare agents currently used in the Czech Armed Forces, eleven compounds were assayed for their free radical scavenging activity. An optimized PC-controlled sequential injection analysis (SIA) system with spectrophotometric detection was used for evaluation of the antioxidation activity of the antidotes. The automated method is based on the known reaction of 2,2´- diphenyl-1-picrylhydrazyl radical (DPPH) with antioxidants. Only the radical scavenging activity of the tested compound 2,3-dimercaptopropanol (BAL) was found to be weaker compared to the reference antioxidant acidum ascorbicum. Other tested antidotes did not possess any DPPH radical scavenging activity. The results obtained show that a correlation between the required biological activity (prophylaxis or treatment of intoxications by warfare agents) and possible antioxidation activity of the tested antidotes is doubtful.
antioxidant; radical; analysis; antidote; warfare agent
Aruoma OI: Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol 32:671-683, 1994.
Aruoma OI: Methodological considerations for characterizing potential antioxidant actions of bioactive components in plant foods. Mutat Res 523-524:9-20, 2003.
Bajgar J: Organophosphates/nerve agents poisoning: mechanism of action, diagnosis, prophylaxis and treatment. Adv Clin Chem 38:151-216, 2004.
Blokhina O, Virolainen E, Fagerstedt KV: Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot (Lond.) 91:179-94, 2003.
Bondet V, Brand-Williams W, Berset C: Kinetics and Mechanisms of Antioxidant Activity using the DPPH Free radical method. Food Sci Technol 30:609-615, 1997.
Cantuti-Castelvetri I, Shukitt-Hale B, Joseph JA: Neurobehavioral aspects of antioxidants in aging. Int. J. Dev. Neurosci. 18:367-381, 2000. Halliwell B: How to characterize a biological antioxidant. Free Radic Res Commun 9:1-32, 1990.
Hollman PCH, Katan MB: Dietary flavonoids: intake, health effects and bioavailability. Food Chem Toxicol 37:937-942, 1999.
Jun D, Kuca K, Hronek M, Opletal L: Effect of some acetylcholinesterase reactivators on human platelet aggregation in vitro. J Appl Toxicol 26:258-61, 2006.
Paulova H, Bochorakova H, Taborska E: In vitro methods for estimation of the antioxidant activity of natural compounds. Chem Listy 98:174-179, 2004.
Polasek M, Skala P, Opletal L, Jahodar L: Rapid automated assay of anti-oxidation/radicalscavenging activity of natural substances by sequential injection technique (SIA) using spectrophotometric detection. Anal Bioanal Chem 379:754-758, 2004.
Ruzicka J, Marshall GD: Sequential injection: A new concept for chemical sensors, process analysis and laboratory assays. Anal Chim Acta 237:329-343, 1990.
Surh YJ, Chun KS, Cha HH, Keum YS, Park KK, Lee SS: Molecular mechanisms underlying chemopreventive activities of antiinflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappaB activation. Mutat Res 480-481:243-268, 2001.
Vaya J, Aviram M: Nutritional antioxidants mechanisms of action, analyses of activities and medical applications. Curr Med Chem 1:99-117, 2001.
Al Moutaery M, Al Rayes H, Al Swailam R, Elfaki I, Khan HA, Alhomida AS, Arshaduddin M, Tariq M. 2,3-Dimercaptopropanol, a thiol chelator, alleviates gastroduodenal ulcers in rats. Fundam Clin Pharmacol. 26: 402-409, 2012.
Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 48: 412-422, 2011.
Zilic S, Maksimovic Dragisic J, Maksimovic V, Maksimovic M, Basic Z, Crevar M, Stankovic G. The content of antioxidants in sunflower seed and kernel. Helia. 33: 75-84, 2010.
Giorgioni G, Claudi F, Ruggieri S, Ricciutelli M, Palmieri GF, Stefano AD, Sozio P, Cerasa LS, Chiavaroli A, Ferrante C, Orlando G, Glennon RA. Design, synthesis, and preliminary pharmacological evaluation of new imidazolinones as l-DOPA prodrugs. Bioorg Med Chem. 18: 1834-1843, 2010.
Zilic S, Sukalovic VHT, Milasinovic M, Ignjatovic-Micic D, Maksimovic M, Semencenko V. Effect of micronisation on the composition and properties of the flour from white, yellow and red maize. Food Technol Biotechnol. 48: 198-206, 2010.
Koleckar V, Opletal L, Macakova K, Jahodar L, Jun D, Kunes J, Kuca K. New antioxidant flavonoid isolated from Leuzea carthamoides. J Enzyme Inhib Med Chem. 25: 143-145, 2010.
Zilic S, Sukalovic VHT, Srebric M, Dodig D, Maksimovic M, Drinic SM, Crevar M. Chemical compositions as quality parameters of ZP soyben and wheat genotypes. Genetika-Belgrade. 41: 297-308, 2009.
Kravic N, Andelkovic V, Sukalovic VHT, Vesna HT, Vuletic M. Antioxidant Activity in Seeds of Maize Genotypes with Different Percentage of Exotic Germplasm. Genetika-Belgrade. 41: 21-28, 2009.
Cervenka F, Koleckar V, Rehakova Z, Jahodar L, Kunes J, Opletal L, Hyspler R, Jun D, Kuca K. Evaluation of natural substances from Evolvulus alsinoides L. with the purpose of determining their antioxidant potency. J Enzyme Inhib Med Chem. 23: 574-578, 2008.
Koleckar V, Opletal L, Brojerova E, Rehakova Z, Cervenka F, Kubikova K, Kuca K, Jun D, Polasek M, Kunes J, Jahodar L. Evaluation of natural antioxidants of Leuzea carthamoides as a result of a screening study of 88 plant extracts from the European Asteraceae and Cichoriaceae. J Enzyme Inhib Med Chem. 23: 218-224, 2008.