Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 5 (2007), No 4, p 195-207




Degenerative action on mice and rat testes of polyspermine and its complexes with RNase A

Josef Matousek, Milan Tomanek, Francesca Vottariello, Manuela Morbio, Giovanni Gotte, Massimo Libonati

Address: Josef Matousek, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov 277 21, Czech Republic
massimo.libonati@univr.it

Received 6th September 2007.
Revised 5th October 2007.
Published online 26th October 2007.

Full text article (pdf)

SUMMARY
A significant aspermatogenic activity, ascertained by microscopic studies of seminiferous tubules and interstitial tissue, and by the observation of the entrance of immunity and fibrocytic cells in mice injected with polyspermine (PS) or polyspermine conjugated to monomeric or dimeric RNase A (PS-RNase A or PS-dimeric RNase A, respectively), was found either in mice injected or in non-injected testes. Polyspermine and its complexes with RNase A destroyed all spermatogenic and intertestitial tissue, including Leydic cells, as well as their ability to secrete testosterone. The total loss of spermatogenic activity in injected testes is irreversible because spermatogonia cells also were destroyed. The injection of PS into both mice testes determined the total degeneration of testicle tissue in 50% of injected testes. The second half of testes was also partly degenerated, and if they were re-injected, almost all testes were fully destroyed. PS-dimeric RNase A injected once into both testicles produced a stronger degeneration and also the interruption of testosterone secretion in comparison with the effects due to injection of mice with PS or PS-RNase A. In all mice treated with these substances, as well as in rats in which PS was injected twice into their testes, we detected polymorfonucleates, monocytes, plasma cells, lymphocytes and fibrocytic cells. Antibodies against PS, PS-RNase A or PS-dimeric RNase A did not influence the aspermatogenic activity. Animals in which a repeated intra-peritoneal injection was carried out did not lose body mass and remained in good condition, with the exception of mice injected with spermine.

KEY WORDS
aspermatogenesis; cell degeneration; testes; spermine; polyspermine; polyspermine-RNase A


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