ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 6 (2008), No 1, p 27-30

Optimization of acetylcholinesterase immobilization onto screen printed platinum electrode

Miroslav Pohanka, Kamil Kuca, Daniel Jun

Address: Miroslav Pohanka, Centre of Biological Defense, Central Military Institute of Health, Techonin, Czech Republic

Received 8th October 2007.
Revised 1st November 2007.
Published online 24th November 2007.

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This article is focused on an optimization of acetylcholinesterase immobilization on screen printed platinum electrodes. An acetylcholinesterase layer cross linked by glutaraldehyde and another non cross linked were compared according to several parameters including background current, current before and current after inhibition by paraoxon. The percentage of inhibition was also calculated. The results obtained confirmed the importance of glutaraldehyde cross linking in the design of the acetylcholinesterase based biosensor.

acetylcholinesterase; immobilization; cross linking; biosensor; electrochemical

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