Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 6 (2008), No 1, p 27-30




Optimization of acetylcholinesterase immobilization onto screen printed platinum electrode

Miroslav Pohanka, Kamil Kuca, Daniel Jun

Address: Miroslav Pohanka, Centre of Biological Defense, Central Military Institute of Health, Techonin, Czech Republic
rau@atlas.cz

Received 8th October 2007.
Revised 1st November 2007.
Published online 24th November 2007.

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SUMMARY
This article is focused on an optimization of acetylcholinesterase immobilization on screen printed platinum electrodes. An acetylcholinesterase layer cross linked by glutaraldehyde and another non cross linked were compared according to several parameters including background current, current before and current after inhibition by paraoxon. The percentage of inhibition was also calculated. The results obtained confirmed the importance of glutaraldehyde cross linking in the design of the acetylcholinesterase based biosensor.

KEY WORDS
acetylcholinesterase; immobilization; cross linking; biosensor; electrochemical


REFERENCES
Andreescu S, Magearu V, Lougarre A, Fournier D, Marty JL: Immobilization of enzymes on screen-printed sensors via an histidine tail. Application to the detection of pesticides using modified cholinesterase. Anal Lett 34:429-540, 2001.

Ciucu A, Ciucu C: Organic phase amperometric biosensor for detection of pesticides. Roum Biotechnol Lett 7:667-676, 2002.

Devic E, Li D, Dauta A, Henriksen P, Codd GA, Marty JL, Fournier D: Detection of anatoxin-a(s) in environmental samples of cyanobacteria by using a biosensor with engineered acetylcholinesterase. Appl Environ Microbiol 68:4102-4106, 2002.

Kuca K, Patocka J, Cabal J: Reactivation of organophosphate inhibited acetylcholinesterase activity by alpha, omega-bis-(4-hydroxyiminomethyl-pyridinium)alkanes in vitro. J Appl Biomed 1:207-211, 2003.

Margariti MG, Tsakalof AK, Tsatsakis AM: Analytical methods of biological monitoring for exposure to pesticides: recent update. Ther Drug Monit 29:150-163, 2007.

Musilek K, Kuca K, Jun D, Dolezal M: In vitro reactivation potency of bispyridinium (E)-but-2-ene linked acetylchlinesterase reactivators against tabun-inhibited acetylcholinesterase. J Appl Biomed 5:25-30, 2007.

Patocka J, Kuca K, Jun D: Acetylcholinesterase and butyrylcholinesterase - important enzymes of human body. Acta Medica (Hradec Kralove) 47:215-228, 2004.

Pohanka M, Jun D, Kuca K: Amperometric biosensor for evaluation of competitive cholinesterase inhibition by the reactivator HI-6. Anal Lett 40:2351-2359, 2007.

Schulze H, Vorlova S, Villatte F, Bachmann TT, Schmid RD: Design of acetylcholinesterases for biosensor applications. Biosens Bioelectron 18:201-209, 2003.

Skladal P: Compensation of temperature variations disturbing performance of an amperometric biosensor for continuous monitoring. Sens Actuat B Chem 28:59-62, 1995.

Skladal P: Biosensors based on cholinesterase for detection of pesticides. Food Technol Biotechnol 34:43-49, 1996.

Skladal P, Krejci J: Performance of the amperometric biosensor with immobilized butyrylcholinesterase in organic solvents. Collect Czech Chem Commun 61:985-991, 1996.

Skladal P, Nunes GS, Yamanaka H, Ribeiro ML: Detection of carbamate pesticides in vegetable samples using cholinesterase-based biosensors. Electroanalysis 9:1083-1087, 1997.

Sotiropoulou S, Chaniotakis NA: Lowering the detection limit of the acetylcholinesterase biosensor using a nanoporous carbon matrix. Anal Chim Acta 530:199-204, 2005.

Vesela S, Ondruska V, Kuca K, Patocka J: Test with Daphnia magna: A new approach to prescreen toxicity of newly synthesized acetylcholinesterase reactivators. J Enzyme Inhib Med Chem 21:427-432, 2006.

Wiesner J, Kriz Z, Kuca K, Jun D, Koca J: Acetylcholinesterases - the structural similarities and differences. J Enzyme Inhib Med Chem 22:417-424, 2007.
CITED

Pohanka M, Zdarova Karasova J, Kuca K, Pikula J, Holas O, Korabecny J, Cabal J: Colorimetric dipstick for assay of organophosphate pesticides and nerve agents represented by paraoxon, sarin and VX. Talanta 81:621-624, 2010.

Pohanka M, Vlcek V, Kuca K, Bandouchova H, Pikula J: Pesticide sorption in typical Central European soils evaluated using a photometric microplate assay based on acetylcholinesterase inhibition. J Appl Biomed 8:41-46, 2010.

Pohanka M, Malir F, Roubal T, Kuca K: Detection of Aflatoxins in Capsicum Spice Using an Electrochemical Immunosensor. Anal Lett 41:2344-2353, 2008.


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