This article is focused on an optimization of acetylcholinesterase immobilization on screen printed platinum electrodes. An acetylcholinesterase layer cross linked by glutaraldehyde and another non cross linked were compared according to several parameters including background current, current before and current after inhibition by paraoxon. The percentage of inhibition was also calculated. The results obtained confirmed the importance of glutaraldehyde cross linking in the design of the acetylcholinesterase based biosensor.
acetylcholinesterase; immobilization; cross linking; biosensor; electrochemical
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