ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 6 (2008), No 1, p 19-26

Antihyperglycaemic effect of Cardiospermum halicacabum Linn. leaf extract on STZ-induced diabetic rats

Chinnadurai Veeramani, Ganesan Pushpavalli, Kodukkur Viswanathan Pugalendi

Address: Kodukkur Viswanathan Pugalendi, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamilnadu, India

Received 24th May 2007.
Revised 10th September 2007.
Published online 31st October 2007.

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The present study was designed to investigate the antihyperglycaemic effect of ethanolic extract of Cardiospermum halicacabum Linn. (Sapindaceae) leaves on normal and streptozotocin (STZ) diabetic rats. Diabetes was induced into male albino Wistar rats by intraperitonial administration of STZ. The Cardiospermum halicacabum leaf extract (CHE) was administered orally at three different doses to normal and STZ-diabetic rats for 45 days. The diabetic rats showed an increase in levels of blood glucose and glycosylated haemoglobin (HbA1c) and a decrease in the levels of insulin and haemoglobin (Hb). In addition, diabetic rats showed a significant reduction in the activity of glucokinase and an elevation in the activities of gluconeogenic enzymes such as glucose-6-phosphatase and fructose-1, 6-bisphosphatase. Treatment with CHE significantly decreased plasma glucose and HbA1c, and increased the levels of insulin and Hb. CHE administration to diabetic rats reversed these enzyme activities in a significant manner. Thus, the results show that CHE possesses an antihyperglycaemic activity and provide evidence for its traditional usage in the control of diabetes. The 200 mg dose of the extract produced a better effect than 50 or 100 mg doses.

Cardiospermum halicacabum; antihyperglycaemia; streptozotocin; insulin; carbohydrate metabolizing enzymes

Alberti KGMM, Press CM: The Biochemistry and Complications of Diabetes. In Keen H, Javve J (eds.): Edward Arnold Publishers, London 1982, pp. 231-270.

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 30:S42-S47, 2007.

Anonymous: The Wealth of India - A Dictionary of Indian Raw Material and Industrial Products, Raw Materials, Vol. 3, CSIR, New Delhi 1992, pp. 269-271.

Asgary S, Naderi GA, Sarraf-Zadegan N, Vakili R: The inhibitory effects of pure flavonoids on in vitro protein glycosylation. J Herb Pharm 2:47-55, 2002.

Baquer NZ, Gupta D, Rajo J: Regulations of metabolic pathways in liver and kidney during experimental diabetes. Effect of antidiabetic compounds. Indian J Clin Biochem 13:63-80, 1998.

Benedict SR: The detection and estimation of glucose in urine. J Am Med Assoc 57:1193-1196, 1911.

Berg JM, Tymoczko JL, Stryer L: Glycolysis and gluconeogenesis. In Berg JM, Tymoczko JL, Stryer L. (eds.): Biochemistry, WH Freeman and Company, New York 2001, pp. 425-464.

Brandstrup N, Kirk JE, Bruni C: Determination of hexokinase in tissues. J Gerontol 12:166-171, 1957.

Burgi W, Briner M, Franken N, Kessler AC: One-step sandwich enzyme immuno assay for insulin using monoclonal antibodies. Clin Biochem 21:311-314, 1988.

Carini M, Adlini G, Furlanetto S, Stefani R, Facino RM: LC coupled to ion-trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas. J Pharm Biomed 24:517-526, 2001.

Chatterjee MN, Shinde R: Textbook of Medical Biochemistry. Metabolism of Carbohydrates, JayPee Medical Publishers, Delhi 2000. p. 421.

Chen V, Ianuzzo CD: Dosage effect of streptozotocin on rat tissue enzyme activities and glycogen concentration. Can J Physiol Pharmacol 60:1251-1256, 1982.

Czinner E, Hagymasi K, Blazovies A, Kery A, Szoke E, Lemberkovics E: In vitro antioxidant properties of Helichrysum arenarium (L.) Moench J Ethnopharmacol 73:437-443, 2000.

Djrolo F, Houngbe H, Avode G, Addra B, Kodjoh N, Avinadje M, Monterio B: Le diabete lie a la malnutrition (diabete tropical). Med Afr Noire 45:538-542, 1998.

Drabkin DL, Austin JM: Spectrophotometric constants for common haemoglobin derivatives in human, dog and rabbit blood. J Biol Chem 98:719-733, 1932.

Gabbay KH: Glycosylated haemoglobin and diabetic control. N Engl J Med 295:443-444, 1976.

Gancedo JM, Gancedo C: Fructose-1,6-bisphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and non-fermenting yeast. Arch Microbiol 76:132-138, 1971.

Ganesan K, Gani SB, Arunachalam GM, Moses RP: Antihyperglycaemic and antiperoxidative effect of Helicteres igora L. bark extracts in streptozotocin-induced diabetic rats. J Appl Biomed 5:97-104, 2007.

Grover JK, Vats V, Rathi SS: Antihyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol 73:461-470, 2000.

Guignot L, Mithieux G: Mechanisms by which insulin, associated or not with glucose, may inhibit hepatic glucose production in the rate. Am J Physiol 277: E984-E989, 1999.

Kamtchouing P, Sokeng DS, Moundipa FP, Watcho P, Jatsa BH, Lontsi D: Protective role of Anacardium occidentale extract against streptozotocin-induced diabetes in rats. J Ethnopharmacol 62:55-99, 1998.

Koeing RJ, Peterson CM, Jones RL: Correlation of glucose regulation and haemoglobin ALC in diabetes mellitus. N Engl J Med 295:417-420, 1976.

Koide H, Oda T: Pathological occurrence of glucose-6-phosphate in serum liver diseases. Clin Chim Acta 4:554-561, 1992.

Latner A: Clinical Biochemistry, Saunders, Philadelphia 1958, p. 48.

Matsuda H, Cai H, Kubo M, Tosa H, Iinuma M: Study on anti-cataract drugs from natural sources. II. Effects of Buddlejae Flos on in vitro aldose reductase activity. Biol Pharm Bull 18:463-466, 1995.

Matsuda H, Morikawa T, Yoshikawa M: Antidiabetogenic constituents from several natural medicines. Pure Appl Chem 74:1301-1308, 2002.

Murray RK, Granner DK, Mayes PA, Rodwell VW: Harper’s Biochemistry, 25th edn., Appleton and Lange, Stanford 2000, pp. 610-617.

Nevwinger HD: African Traditional Medicine: A Dictionary of Plant Use and Applications. Medpharm GmbH Scientific Publishers, Stuttgart 2000, pp. 1-300.

Paulsen EP: Haemoglobin ALC in childhood of diabetes. Metabolism 22:269-271, 1973.

Romanova D, Vachalkova A, Cipak I, Ovesna Z, Rauko P: Study of antioxidant effect of apigenin, luteolin and quercetin by DNA protective method. Neoplasma 48:104-107, 2001.

Schein PS, Cooney DA, McMenamin MG, Anderson T: Streptozotocin diabetes-further studies on the mechanism of depression of nicotinamide adenine dinucleotide concentrations in mouse pancreatic islets and liver. Biochem Pharmacol 22:2625-2631, 1973.

Sudhakar NS, Pattabiraman TN: A new colorimetric method for the estimation of glycosylated haemoglobin. Clin Chim Acta 109:267-274, 1981.

The European Agency for the Evaluation of Medicinal Products (TEAEMP). Veterinary medicines evaluation unit. EMEA/MRL/664/99-FINAL, 1999.

Trinder P: Determination of blood glucose using an oxidase peroxidase system with a non-carcinogenic chromogen. Ann Clin Biochem 6:24-30, 1969.

Ueda H, Kaneda N, Kawanishi K, Alves SM, Moriyasu M: A new isoflavone glycoside from Ceiba pentandra (L). Gaertner Chem Pharm Bull 50:403-404, 2002.

WHO: WHO launches the first global strategy on traditional medicine, Press Release WHO/38, 2002.

Jeyadevi R, Sivasudha T, Rameshkumar A, Harnly JM, Lin LZ. Phenolic profiling by UPLC-MS/MS and hepatoprotective activity of Cardiospermum halicacabum against CCl4 induced liver injury in Wistar rats. J Funct Foods. 5: 289-298, 2013.

Jeyadevi R, Sivasudha T, Rameshkumar A, Kumar LD. Anti-arthritic activity of the Indian leafy vegetable Cardiospermum halicacabum in Wistar rats and UPLC-QTOF-MS/MS identification of the putative active phenolic components. Inflamm Res. 62: 115-126, 2013.

Ramachandran S, Naveen KR, Rajinikanth B, Akbar M, Rajasekaran A. Antidiabetic, antihyperlipidemic and in vivo antioxidant potential of aqueous extract of Anogeissus latifolia bark in type 2 diabetic rats. Asian Pacific J Tropical Dis. 2(Suppl. 2): S596-S602, 2012.

Oak M, Singh J. Chitosan-zinc-insulin complex incorporated thermosensitive polymer for controlled delivery of basal insulin in vivo. J Controlled Release. 163: 145-153, 2012.

Veeramani C Al-Numair KS, Alsaif MA, Chandramohan G, Al-Numair NS, Pugalendi KV. Protective effect of Cardiospermum halicacabum leaf extract on glycoprotein components on STZ-induced hyperglycemic rats. Asia Pacific J Tropical Med. 5: 939-944, 2012.

Parameshappa B, Basha MSA, Sen S, Chakraborty R, Kumar GV, Sagar GV, Sowmya L, Raju KK, Kumar PKRS, Lakshmi AVSM. Acetaminophen-induced nephrotoxicity in rats: Protective role of Cardiospermum halicacabum. Pharm Biol. 50: 247-253, 2012.

Ramachandran S, Asokkumar K, Uma Maheswari M, Ravi TK, Sivashanmugam AT, Saravanan S, Rajasekaran A, Dharman J. Investigation of antidiabetic, antihyperlipidemic, and in vivo antioxidant properties of Sphaeranthus indicus Linn. in type 1 diabetic rats: An identification of possible biomarkers. Evidence-based Complementary and Alternative Medicine. 1-8, 2011.

Pareek A, Suthar M. Antidiabetic activity of extract of Berberis aristata root in streptozotocin induced diabetic rats. Pharmacologyonline. 2: 179-185, 2010.

De D, Chatterjee K, Ali KM, Mandal S, Barik B, Ghosh D. Antidiabetic and antioxidative effects of hydro-methanolic extract of sepals of Salmalia malabarica in streptozotocin induced diabetic rats. J Appl Biomed. 8: 23-33, 2010.