ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 6 (2008), No 2, p 73-79

Locomotor activity and serum tryptophan and serotonin in goats: daily rhythm

Giuseppe Piccione, Claudia Giannetto, Anna Assenza, Francesco Fazio, Giovanni Caola

Address: Giuseppe Piccione, Laboratorio di Cronofisiologia Veterinaria, Facolta di Medicina Veterinaria, Universita di Messina, Polo Universitario dell’Annunziata, 98168 Messina - Italy.

Received 20th February 2008.
Revised 27th February 2008.
Published online 22nd April 2008.

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The goal of the present study was to investigate the daily amount of motor activity and the daily rhythm of tryptophan and serotonin in goats housed in individual boxes under a 12/12 light/dark cycle. We equipped six Girgentana breed goats with Actiwatch-Mini (Cambridge Neurotechnology Ltd, UK), actigraphy-based data loggers that record a digitally integrated measure of motor activity. Also blood samples were collected at four-hour intervals over a 48 h period via an intravenous cannula inserted into the jugular vein. The concentration of tryptophan (TRP) and serotonin (5-HT) were assessed by the high-performance liquid chromatography (HPLC) method. Our results outline a diurnal pattern of motor activity and levels of tryptophan in the serum, and a nocturnal pattern of levels of serotonin in the serum, which underline the impact of endogenous serotoninergic activity on the behavioural aspects of the circadian response to light.

motor activity; tryptophan; serotonin; daily rhythm; Capra hircus

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