Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 6 (2008), No 4, p 195-201




Changes in selected parameters of the antioxidant system in radiation damage to the organism

Leos Navratil, Jaroslav Racek, Renata Havrankova, Ladislav Beranek, Zuzana Freitinger Skalicka, Hana Siffnerova, Eva Kantorova

Address: Leos Navratil, University of South Bohemia, Faculty of Health and Social Studies, Department of Radiology and Toxicology, Matice skolske 17, CZ 370 01 Ceske Budejovice, Czech Republic
leos.navratil@volny.cz

Received 1st October 2008.
Revised 8th December 2008.
Published online 12th December 2008.

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SUMMARY
The target of the present work was a study of the oxidation-reduction system of the organism damaged with ionizing radiation. The parameters chosen for examination were: the superoxide dismutase activity in erythrocytes, the concentration of reduced glutathione in erythrocytes, the activity of glutathione peroxidase in the total blood, the concentration of malondialdehyde in the blood plasma, and the antioxidant capacity of blood plasma. These parameters were monitored in four groups (patients after radiotherapy, volunteers whose blood was submitted to in vitro irradiation, employees of a nuclear power plant, and a group of healthy persons). A statistically significant decrease in the GSHPx activity was measured in patients after radiotherapy and in employees of the nuclear power plant. Results of processing the data obtained by the method of multidimensional analysis of variance suggest that the dynamics of the changes is comparable in the group of patients after radiotherapy and employees of the nuclear power plant on the one hand, and in the control group and group of healthy volunteers whose blood was subjected to in vitro irradiation, on the other. The study demonstrated changes concerning certain parameters of the oxidation-reduction system after the action of ionizing radiation.

KEY WORDS
antioxidant system; blood plasma; ionizing radiation


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