Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 6 (2008), No 4, p 177-186




The effect of tryptophanadministration on the circadian rhythms of melatonin in plasma and the pineal gland of rats

Soledad Sanchez, Cristina Lucia Sanchez, Sergio Damian Paredes, Ana Beatriz Rodriguez and Carmen Barriga

Address: Soledad Sanchez Mateos, Department of Physiology, Faculty of Science, University of Extremadura, Avda de Elvas s/n, 06071 Badajoz, Spain
solsan@unex.es

Received 4th September 2008.
Revised 6th November 2008.
Published online 28th November 2008.

Full text article (pdf)

SUMMARY
Most physiological processes that take place in the body have a circadian rhythm which is controlled by an internal biological clock located in the suprachiasmatic nucleus. The indole melatonin synthesized in the pineal gland, acts to synchronize these biological rhythms, and also it is synthesized and released following a circadian rhythm. The present study analyzed the levels of melatonin over a 24-hour period in Wistar rats in both basal and control conditions and after the oral administration of 125 mg/kg tryptophan, the amino acid that is the precursor of this indole, for 7 days. The levels of melatonin in the plasma and the pineal gland were measured by radioimmunoassay every hour during the night, and every 4 hours during the day. The results indicated that the tryptophan administration provoked raised levels of melatonin at all hours studied in both plasma and pineal. Of the chronobiological parameters studied, there were also increases in the values of the melatonin MESOR with respect to the values obtained in the basal and control groups (the respective increases being 45% and 52% in plasma, and 46% and 47% in the pineal), as well as an advanced acrophase with respect to the basal and control groups. In summary, our findings confirm that tryptophan intake one hour before lights-off increases melatonin levels in plasma and pineal over a 24-hour period, as well as advancing the peak of its synthesis.

KEY WORDS
circadian rhythms; melatonin; pineal gland; plasma; rat; tryptophan


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CITED

Delgado J, Terron MP, Garrido M, Pariente JA, Barriga C, Rodriguez AB, Paredes SD. Diets enriched with a Jerte Valley cherry-based nutraceutical product reinforce nocturnal behaviour in young and old animals of nocturnal (Rattus norvegicus) and diurnal (Streptopelia risoria) chronotypes. J Anim Physiol Anim Nutr. 97: 137-145, 2013.

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Delgado J, Terron MP, Garrido M, Pariente JA, Barriga C, Rodriguez AB, Paredes SD. A cherry nutraceutical modulates melatonin, serotonin, corticosterone, and total antioxidant capacity levels: effect on ageing and chronotype. J Appl Biomed. 10: 109-117, 2012.

Delgado J, Terron MP, Garrido M, Barriga C, Espino J, Paredes SD, Rodriguez AB. Jerte Valley cherry-based product modulates serum inflammatory markers in rats and ringdoves. J Appl Biomed. 10: 41-50, 2012.

Bejarano I, Espino J, Marchena AM, Barriga C, Paredes SD, Rodriguez AB, Pariente JA. Melatonin enhances hydrogen peroxide-induced apoptosis in human promyelocytic leukaemia HL-60 cells. Mol Cell Biochem. 353: 167-176, 2011.

Emadi M, Jahanshiri F, Kaveh K, Hair-Bejo M, Ideris A, Alimon AR. Nutrition and immunity: the effects of the combination of arginine and tryptophan on growth performance, serum parameters and immune response in broiler chickens challenged with infectious bursal disease vaccine. Avian Pathol. 40: 63-72, 2011.

Ortiz A, Espino J, Bejarano I, Lozano GM, Monllor F, Garcia JF, Pariente JA, Rodriguez AB. The correlation between urinary 5-hydroxyindoleacetic acid and sperm quality in infertile men and rotating shift workers. Reprod Biol Endocrinol. 8, art. no. 138, 2010.

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Garrido M, Espino J, Gonzalez-Gomez D, Lozano M, Cubero J, Toribio-Delgado AF, Maynar-Marino JI, Terron MP, Munoz JL, Pariente JA, Barriga C, Paredes SD, Rodriguez AB. A nutraceutical product based on Jerte Valley cherries improves sleep and augments the antioxidant status in humans. e-SPEN. 4: e321-e323, 2009.

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