Journal of APPLIED BIOMEDICINE
ISSN 1214-0287 (on-line)
ISSN 1214-021X (printed)

Volume 7 (2009), No 2, p 101-109




Glutathione, glutathione-dependent enzymes and antioxidant status in gastric carcinoma patients

Palanisamy Pasupathi, Ganesan Saravanan, Palanisamy Chinnaswamy, Govindaswamy Bakthavathsalam

Address: Palanisamy Pasupathi, Head-Department of Clinical Biochemistry, Institute of Laboratory Medicine, K. G. Hospital and Post Graduate Medical Institute, Coimbatore-641 018, Tamil Nadu, India
drppasupathi@gmail.com

Received 24th September 2008.
Revised 5th January 2009.
Published online 15th March 2009.

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SUMMARY
Oxidative stress plays an important role in malignant transformation and is postulated to be associated with increased lipid peroxidation. The aim of this study was to determine the extent of lipid peroxidation with the antioxidant status in patients with gastric cancer. The study population consisted of fifty newly diagnosed gastric cancer patients and an equal number of age- and sex-matched healthy control subjects. Lipid peroxidation as evidenced by thiobarbutric acid reactive substances (TBARS), and also the status of enzymatic (superoxide dismutase - SOD, catalase - CAT, glutathione peroxidase - GPx, glutathione reductase - GR, glutathione S-transferase - GST) and non-enzymatic (reduced glutathione - GSH) antioxidants, were determined. Enhanced lipid peroxidation with concomitant depletion of antioxidants was observed in gastric cancer patients as compared to healthy control subjects. The present study highlights the occurrence of lipid peroxidation and possible breakdown of antioxidant status in patients with gastric carcinoma.

KEY WORDS
gastric cancer; lipid peroxidation; antioxidants; glutathione; GSH-dependent enzymes


REFERENCES
Abou-Ghalia AH, Found IM: Glutathione and its metabolizing enzymes in patients with different benign and malignant diseases. Clin Biochem 33:657-662, 2000.

Arivazhagan S, Kavitha K, Nagini S: Erythrocyte lipid peroxidation and antioxidants in gastric cancer patients. Cell Biochem Funct 15:15-18, 1997.

Bakan E, Taysi S, Polat MF, Dalga S, Umudum Z, Bakan N, Gumus M: Nitric oxide levels and lipid peroxidation in plasma of patients with gastric cancer. Jpn J Clin Oncol 32:162-166, 2002.

Beutler E, Kelley BM: The effect of sodium nitrate on red cell glutathione. Experientia 19:96-97, 1963.

Bjelakovic G, Nikolova D, Simonetti RG, Gluud C: Antioxidant supplements for prevention of gastrointestinal cancers: a systematic review and meta-analysis. Lancet 364:1219-1228, 2004.

Boisio ML, Merlo F, Martines H, Esposito M: Blood levels of reduced and oxidized glutathione in malignant and non-malignant human colorectal lesions. Eur J Cancer 26:89-91, 1990.

Carlberg I, Mannervik B: Glutathione reductase, In: Meister A (ed.): Methods in enzymalogy, Accademic Press, New york 1985, pp 484-490.

Codandabany C: Erythrocyte lipid peroxidation and antioxidants in cigarette smokers. Cell Biochem Funct 18:99-102, 2000.

Cotton SC, Sharp L, Little J, Brockton N: Glutathione S-transferase polymorphisms and colorectal cancer. Am J Epidemiol 151:7-32, 2000.

Della Rovere F, Granata A, Saija A, Broccio M, Tomaino A, Zirilli A, De Caridi G, Broccio G: SH groups and glutathione in cancer patient's blood. Anticancer Res 20:1595-1598, 2000.

Donnan SK: The thiobarbituric acid test applied to tissues from rats treated in various ways. J Biol Chem 182:415-419, 1950.

Dreher D, Junod AF: Role of oxygen free radicals in cancer development. Eur J Cancer 32:30-38, 1996.

Ellman GL: Tissue sulfhydryl groups. Arch Biochem Biophys 82:70-77, 1959.

Estrela JM, Ortega A, Obrador E: Glutathione in cancer biology and therapy. Crit Rev Clin Lab Sci. 43:143-181, 2006.

Forman D, Burley VJ: Gastric cancer: global pattern of the disease and an overview of environmental risk factors. Best Pract Res Clin Gastroenterol 20:633-649, 2006.

Gerber M, Astre C, Segala C, Saintot M, Scali J, Simony-Lsfontaine J: Oxidant-antioxidant status alterations in cancer patients: relationship to tumour progression. J Nutr 126:1201, 1996.

Gupta A, Butts B, Kwei KA, Dvorakova K, Stratton SP, Briehl MM, Bowden GT: Attenuation of catalase activity in the malignant phenotype plays a functional role in an in vitro model for tumour progression, Cancer Lett 173:115-125, 2001.

Habig WH, Pabst MJ, Jakoby WB: Glutathione-S-transferases, the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130-7139, 1974.

Halliwell B, Gutteridge JMC: Oxygen free radicals and iron in relation to biology and medicine: Some problems and concepts. Arch Biochem Biophys 246:501-514, 1986. Hayes JD, McLellan LI: Glutathione and glutathione-dependent enzymes represent a coordinately regulated defence against oxidative stress. Free Radic Res 31:273-300, 1999.

Ho JC-M, Zheng S, Comhair SAA, Farver C, Erzurum SC: Differential expression of manganese superoxide dismutase and catalase in lung cancer. Cancer Res 61:8578-8585, 2001.

Hussain SP, Hofseth LJ, Harris CC: Radical causes of cancer. Nat Rev Cancer 3:276-285, 2003.

Hwang TS, Choi HK, Han HS: Differential expression of manganese superoxide dismutase, copper/zinc superoxide dismutase, and catalase in gastric adenocarcinoma and normal gastric mucosa. EJSO 33:474-479, 2007.

Jernstrom B, Morgenstern R, Moldeus P: Protective role of glutathione, thiols, and analogues in mutagenesis as carcinogenesis. Basic Life Sci 61:137-47, 1993.

Johansen JS, Harris AK, Rychly, DJ, Ergul A: Oxidative stress and the use of antioxidants in diabetes: linking basic science to clinical practice. Cardiovasc Diabetol 4:5, 2005.

Kakkar PS, Das B, Viswanathan PN: A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21:130-132, 1984.

Khanzode SS, Khanzode SD, Dakhale GN: Serum and plasma concentration of oxidant and antioxidants in patients of Helicobacter pylori gastritis and its correlation with gastric cancer. Cancer Lett 95:27-31, 2003.

Kim Y, Shin A, Gwack J, Jun J K, Park S K, Kang D, Shin H R, Chang S H, Yoo KY J: Cigarette smoking and gastric cancer risk in a community based cohort study in Korea. Prev Med Pub Health 40:467-474, 2007.

Kireev GV, Balenkov OIU, Sharipov FK, Dzhuraev MD: Lipid peroxidation and tumour dimensions in stomach cancer. Klin Lab Diagn 20:33-34, 2004.

Kumaraguruparan R, Subapriya R, Kabalimoorthy J, Nagini S: Antioxidant profile in the circulation of patients with fibroadenoma and adenocarcinoma of the breast. Clin Biochem 35:275-279, 2002.

Lang CA, Naryshkin S, Schneider DL, Mills BJ, Lindeman RD: Low blood glutathione levels in healthy aging adults. J. Lab Clin Med 120:720-725, 1992.

Laurent A, Nicco C, Chereau C, Goulvestre C, Alexandre J, Alves A, Levy E, Goldwasser F, Panis Y, Soubrane O, Weill B, Batteux F: Controlling tumour growth by modulating endogenous production of reactive oxygen species, Cancer Res 65:948-956, 2005.

Lin CC, Yin MC: B vitamins deficiency and decreased anti-oxidative state in patients with liver cancer. Eur J Nutr 46:293-299, 2007.

Liu TS, Wang Y, Chen SY, Sun YH: An updated meta-analysis of adjuvant chemotherapy after curative resection for gastric cancer. EJSO 34:1208-1216, 2008.

Marnett LJ: Oxy radicals, lipid peroxidation and DNA damage. Toxicology 182:219-222, 2002.

Masella R, Di Benedetto R, Vari R, Filesi C, Giovannini C: Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes. J Nutr Biochem 16:577-586, 2005.

Mates JM, Perez-Gomez C, De Castro IN: Antioxidant enzymes in human diseases. Clin Biochem 32:595-603, 1999.

Niederhofer LJ, Daniels JS, Rauzer CA, Greene RE, Marnett LJ: Malonaldehyde, a product of lipid peroxidation is mutagenic in human cells. J Biol Chem 278:31426-31433, 2003.

Nishikawa M: Reactive oxygen species in tumour metastasis. Cancer Lett 266:53-59, 2008.

Parkin DM, Bray F, Ferlay J, Pisani P: Global cancer statistics. Cancer J Clin 55:74-108, 2005.

Pastore A, Fedrici G, Bertini E, Piemonte F: Analysis of glutathione: implication in redox and detoxification. Clin Chim Acta 33:19-39, 2003.

Pasupathi P, Saravanan G, Bakthavathsalam G: Effect of chronic smoking on erythrocyte lipid peroxidation and antioxidant status in gastric carcinoma patients. J Cell Tissue Research 8:1399-1403, 2008.

Polat MF, Taysi S, Gul M, Cikman O, Yilmaz I, Bakan E: Oxidant/antioxidant status in blood of patients with malignant breast tumour and benign breast diseases. Cell Biochem Funct 20:327-331, 2002.

Robinson ME, Godfrey PJ, Thompson LD, Rea HM and Van Riji AM: Blood selenium and glutathione peroxidase activity in normal subjects and in surgical patients with and without cancer in New Zealand. Am J Clin Nutr 32:1477-1485, 1979.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG: Selenium: biochemical roles as a component of glutathione peroxidase. Science 179:588-590, 1973.

Scibior D, Skrzycki M, Podsiad M, Czeczot H: Glutathione level and glutathione-dependent enzyme activities in blood serum of patients with gastrointestinal tract tumours. Clin Biochem 41:852-858, 2008.

Scott MD, Lubin BH, LinZuo and Kuypers FA: Erythrocyte defence against hydrogen peroxide pre- eminent importance of catalase. J Lab Clin Med 118, 7-16, 1991.

Sen CK: Cellular thiols and redox-regulated signal transduction. Curr Top Cell Regul 36:1-30, 2000.

Sies H: Glutathione and its cellular functions. Free Radic Biol Med 27:916-21, 1999.

Sies H: Oxidative stress: oxidants and antioxidants. Exp Physiol 82:291-295, 1997.

Sinha KA: Colorimetric assay of catalase. Anal Biochem 47:389-394, 1972.

Skrzydlewska E, Kozuszko B, Sulkowska M, Bogdan Z, Kozlowski M, Snarska J, Puchalski Z, Sulkowski S, Skrzydlewski Z: Antioxidant potential in esophageal, stomach and colorectal cancers. Hepatogastroenterology 50:126-131, 2003.

Storz P: Reactive oxygen species in tumour progression. Front Biosci 10:1881-1896, 2005.

Tsuchida S, Sato K: Glutathione transferases and cancer. Crit Rev Biochem Mol Biol 27:337-84, 1992.

Vattem DA, Shetty RK: Cranberry phenolics-mediated antioxidant enzyme response in oxidatively stressed porcine muscle. Process Biochemistry 40:2225-2238, 2004.

Yagi K: Lipid peroxides and human diseases. Chem Phys Lipids 45:337-351, 1978.


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