ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 8 (2010), No 1, p 47-54
DOI 10.2478/v10136-009-0007-7

Inhibition of Ehrlich’s ascites carcinoma by ethyl acetate extract from the flower of Calotropis gigantea L. in mice

Muhammad Rowshanul Habib, Muhammad Abdul Aziz, Muhammad Rezaul Karim

Address: Muhammad Rezaul Karim, Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205, Bangladesh
mrkbio_onco@yahoo.com

Received 29th August 2009.
Revised 27th October 2009.
Published online 18th November 2009.

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SUMMARY
As part of a scientific appraisal of some of the folkloric and ethnomedical uses of Calotropis gigantea L. (Family: Asclepiadaceae), the present study was designed to evaluate the antitumour activity of the Calotropis gigantea flower against Ehrlich’s ascites carcinoma (EAC) by using a crude ethyl acetate extract from the flower of Calotropis gigantea (designated as EECF) in Swiss mice. EECF was administered intraperitoneally at doses of 50, 100 and 200 mg/kg body weight. Bleomycin (0.3 mg/kg) was used as a positive control. EECF treatment significantly decreased both viable tumour cells and body weight gain induced by the tumour burden and prolonged survival time. The haematological and biochemical (glucose, cholesterol, triglyceride, blood urea, ALP, SGPT and SGOT) parameters altered during tumour progression, were also significantly restored in EECF-treated mice. Among the three doses tested, the highest dose was the most potent and comparable with the standard drug bleomycin (0.3 mg/kg). In conclusion, EECF from the Calotropis gigantea flower has a potent inhibitory effect against EAC cells in a dose dependent manner.

KEY WORDS
Calotropis gigantea; Ehrlich’s ascites carcinoma; flower extract; Swiss mice


REFERENCES
Adak M, Gupta JK: Evaluation of anti-inflammatory activity of Calotropis gigantea (AKANDA) in various biological systems. Nepal Med Coll J 8:156-161, 2006.

Ahmed H, Chatterjee BP, Debnath AK: Interaction and in vivo growth inhibition of Ehrlich ascites tumour cells by jacalin. J Biosciences 13:419-424, 1988.

Ajith TA, Janardhanan KK: Cytotoxic and antitumor activities of a polypore macrofungus, Phellinus rimosus (Berk) Pilat. J Ethnopharmacol 109:41-47, 2003.

Argal A, Pathak AK: CNS activity of Calotropis gigantea roots. J Ethnopharmacol 106:142-145, 2006.

Chitme HR, Chandra R, Kaushik S: Studies on anti-diarrhoeal activity of Calotropis gigantea R. Br. in experimental animals. J Pharm Pharm Sci 7:70-75, 2004.

Chitme HR, Chandra R, Kaushik S: Evaluation of antipyretic activity of Calotropis gigantea (Asclepiadaceae) in experimental animals. Phytother Res 19:454-456, 2005.

Fecchio D, Sirois P, Russo M, Jancar S: Studies on inflammatory response induced by Ehrlich tumor in mice peritoneal cavity. Inflammation 14:125-131, 1990.

Ghani A: Medicinal Plants of Bangladesh, Asiatic Society of Bangladesh, Dhaka 2003, pp. 141.

Gibbs JB: Mechanism based target identification and drug discovery in cancer research. Science 287:1967-1973, 2000.

Gupta J, Ali M: Rare chemical constituents from Calotropis gigantea roots. Indian J Pharm Sci 62:29-32, 2000.

Habib MR, Nikkon F, Rahman M, Haque ME, Karim MR: Isolation of stimasterol and beta-sitosterol from methanolic extract of root bark of Calotropis gigantea (linn). Pak J Biol Sci 10:4174-4176, 2007.

Hogland HC: Heamatological complications of Cancer chemotherapy. Seminar in Oncology 9:95-102, 1982.

Kartikar KR, Basu N: Indian Medicinal Plants. Lolit Mohan Basu, Allahabad 1994, pp. 1607.

Killington RA, Williams AE, Ratchffe NA, Whitehead TP: Hypoglycemia in rats bearing ascites tumor. Br J Cancer 25:93-102, 1991.

Kintzios SE: Terrestrial plant derived anticancer agents and plants used in anticancer research. CRC Crit Rev Plant Sci 25:79-113, 2006.

Kitagawa I, Zhang RS, Park JD, Baek NI, Takeda Y, Yoshikawa M, Shibuya H: Indonesian medicinal plants. I. Chemical structures of calotroposides A and B, two new oxypregnane-oligoglycosides A and B, from the root of Calotropis gigantea (Asclepiadaceae). Chem Pharm Bull (Tokyo) 40:2007-2013, 1992.

Kiuchi F, Fukao Y, Maruyama T, Obata T, Tanaka M, Sasaki T, Mikage M, Haque ME, Tsuda Y: Cytotoxic principles of a Bangladeshi crude drug, akond mul (roots of Calotropis gigantea L.). Chem Pharm Bull (Tokyo) 46:528-530, 1998.

Kviecinski MR, Felipe KB, Schoenfelder T, de Lemos Wiese LP, Rossi MH, Goncalez E, Felicio JD, Filho DW, Pedrosa RC: Study of the antitumor potential of Bidens pilosa (Asteraceae) used in Brazilian folk medicine. J Ethnopharmacol 117:69-75, 2008.

Latha PG, Panikkar KR: Cytotoxic and antitumour principles from Ixora coccinea flowers. Cancer Lett 130:197-2002, 1998.

Lhinhatrakool T, Sutthivaiyakit S: 19-Nor- and 18, 20-epoxy-cardenolides from the leaves of Calotropis gigantea. J Nat Prod 69:1249-1251, 2006.

Lodhi G, Singh HK, Plant KK, Hussain Z: Hepatoprotective effects of Calotropis gigantea extract against carbon tetrachloride induced liver injury in rats. Acta Pharm 59:89-96, 2009.

Lora J, Alonso FJ, Segura JA, Lobo C, Marquez J, Mates JM: Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascetic tumour cells. Eur J Biochem 271:4298-4306, 2004.

Lorke DA: A new approach to practical acute toxicity testing. Arch Toxicol 54:275-287, 1983.

Maseki M, Nishiagaki I, Hagishara M, Tomoda Y, Yagi K: Lipid peroxidation levels and lipid content of serum lipoprotein fractions of pregnant subjects with or without preeclamsia. Clin Chim Acta 41:424-426, 1981.

Mates JM, Segura JA, Alonso FJ, Marquez J: Intracellular redox status and oxidative stress: implications for cell proliferation, apoptosis, and carcinogenesis. Arch Toxicol 82:273-299, 2008.

Mates JM, Segura JA, Alonso FJ, Marquez J: Natural Antioxidants: Therapeutic Prospects for Cancer and Neurological Diseases. Mini Rev Med Chem 9:1209-1214, 2009.

Mueen AKK, Rana AC, Kixit VK: Calotropis species (Asclepiadaceae) - A comprehensive review. Pharmacognosy Magazine 1:48-52, 2005.

Mukherjee KL: Medical Laboratory Technology. Tata Mcgraw Hill Publishing Company Limited, New Delhi 1988, pp. 218-280.

Nair SC, Panikkar B, Akamanchi KG, Panikkar KR: Inhibitory effects of Ixora javanica extract on skin chemical carcinogenesis in mice and its antitumour activity. Cancer Lett 60:253-258, 1991.

Nitha B, Meera CR, Janardhanan KK: Anti-inflammatory and antitumor activities of cultured mycelium of morel mushroom, Morchella esculenta. Curr Sci Ind 92:235-239, 2007.

Pari K, Rao PJ, Devakumar C, Rastogi JN: A novel insect antifeedant nonprotein amino acid from Calotropis gigantea. J Nat Prod 61:102-104, 1998.

Pathak AK, Argal A: Analgesic activity of Calotropis gigantea flower. Fitoterapia 78:40-42, 2007.

Ramachandra SS, Quereshi AA, Viswanath SAH, Patil T, Prakash T, Prabhu K, Veeran GA: Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats. Fitoterapia 78:451-454, 2007.

Rajkapoor B, Jayakar B, Murugesh N: Antitumor activity of Bauhinia variegain against Ehrlich Ascites Carcinoma Induced Mice. Pharm Biol 41:604-607, 2003.

Sastri BN: Wealth of India, Raw Materials. Publication and Information Directorate, CSIR, New Delhi 1950, pp. 20.

Segura JA, Barbero LG, Marquez J: Ehrlich ascites tumor unbalances splenic cell population and reduces reponsiveness of T cells to Staphylococcus aureus enterotoxin B stimulation. Immunology Lett. 74:111-115, 2000.

Sen S, Sahu NP, Mahato SB: Flavonol glycosides from Calotropis gigantea. Phytochemistry 31:2919-2921, 1992.

Senthilkumar R, Manivannan R, Balasubramaniam A, Sivakumar T and Rajkapoor B: Effect of ethanol extract of Pisonia aculeate Linn. on ehrlich’s ascites carcinoma tumor bearing mice. Int J Green Pharm 2:50-53, 2008.

Shibuya H, Zhang R, Park JD, Back NI, Takeda Y, Yoshikawa M, Kitagawa I: Chemical structures of Calotroposides C, D, E, F and G, five additional new oxypregnane-oligoglycosides from the root of Calotropis gigantea (Asclepiadaceae). Chem Pharm Bull (Tokyo) 40:2647-2653, 1992.

Shimizu M, Azuma C, Taniguchi T, Murayama T: Expression of Cytosolic Phospholipase A2alpha in murine C12 cells, a variant of L929 cells, induces arachidonic acid release in response to phorbol myristate acetate and Ca2+ ionophores, but not to tumor necrosis factor-alpha. J Pharmacol Sci 96:324-332, 2004.

Silverstein H, Dervot K, Oscar D: Studies on carbohydrate metabolism and different types of tumors bearing animals. Lancet 22:40-45, 1988.

Sonnenwirth AC, Jarett L: Clinical Laboratory Methods and Diagnosis. Mosby CV, St. Louis, 1980, pp. 305-323.

Suffines M, Pezzuto JM: Assays related to cancer drug discovery. In: Methods in Plant Biochemistry. Academic Press, New York, 1991, pp. 72-98.

Sur P, Ganguly DK: Tea plant root extract (TRE) as an antineoplastic agent. Planta Med 60:106-109, 1994.

Tannock IE, Richard P: The Basic Science of Oncology. Mcgraw Hill, New York 1998, pp. 384-385.

Zhu-Nian W, Mao-Yuan W, Wen-Li M, Zhuang H, Hao-Fu D: A new cytotoxic pregnanone from Calotropis gigantea. Molecules 13:3033-3039, 2008.

CITED

Islam F, Ali SMM, Khanam JA. Hepatoprotective effect of acetone semicarbazone on Ehrlich ascites carcinoma induced carcinogenesis in experimental mice. Asia-Pac J Tropic Biomed. 3: 105-110, 2013.

Habib MR, Karim MR. Antitumour evaluation of di-(2-ethylhexyl) phthalate (DEHP) isolated from Calotropis gigantea L. flower. Acta Pharm. 62: 607-615, 2012.

Berger J. The age of biomedicine: current trends in traditional subjects. J Appl Biomed. 9: 57-61, 2011.

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