ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 8 (2010), No 1, p 41-46
DOI 10.2478/v10136-009-0006-8

Pesticide sorption in typical Central European soils evaluated using a photometric microplate assay based on acetylcholinesterase inhibition

Miroslav Pohanka, Vitezslav Vlcek, Kamil Kuca, Hana Bandouchova, Jiri Pikula

Address: Miroslav Pohanka, Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic

Received 16th July 2009.
Revised 11th September 2009.
Published online 26th November 2009.

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Sorption isotherms were estimated for two model organophosphorus pesticides - methamidophos and paraoxon-ethyl - and four typical Central European soils endangered by these pesticides: haplic Chernozems, Cambisols, Luvisols, and Fluvisols. A photometric microplate assay based on the recognition capability of the enzyme acetylcholinesterase toward organophosphorous pesticides was used for the construction of sorption isotherms. The sorption capacity of each soil was then determined and it was found that the soils with the highest content of humic acid (haplic Chernozems and haplic Luvisols) sorbed pesticides most. Pesticides in concentrations over the sorption capacity were easily removed from soil by water.

organophosphates; methamidophos; paraoxon; sorption isotherms; capacity

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