ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 8 (2010), No 2, p 81-86
DOI 10.2478/v10136-009-0012-x

The incidence of beta-defensin-1, 2, 3 in human healthy and chronically inflamed nasal and tonsillar mucosa

Hana Pacova, Jaromir Astl, Jindrich Martinek

Address: Hana Pacova, Inst. of Histology and Embryology, First Faculty of Medicine, Charles University, Albertov 4, 128 01 Prague 2, Czech Republic
Hpac@seznam.cz

Received 26th August 2009.
Published online 23rd March 2010.

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SUMMARY
The nasal and tonsillar mucosa are exposed to massive incursions of pathological microorganisms. One of the mechanisms known to prevent an invasion of pathogens is an endogenous synthesis of antimicrobial peptides, which include human beta-defensins-1, 2, 3 (HBD-1, 2, 3).
The aim of this study was to demonstrate the occurrence of HBD-1, 2 and 3 in the human nasal mucosa and palatine tonsils in healthy tissues and during chronic inflammation (nasal polyposis with and without the colonization of Staphylococcus aureus and chronic tonsillitis) and to evaluate their incidence under varying conditions.
Another target was to compare the occurrence of human beta-defensins in these two different entities; that is, in the nasal mucosa and in the palatine tonsil.
It was assumed that the incidence of HBD-1, 2, 3 was lower in tonsils than in nasal mucosa; however, inflamed samples of tonsils and nasal mucosa showed no difference in the production of HBD-1, 2, 3. The presence of all three subfamilies of HBD was significantly lower in nasal polyps with Staphylococcus aureus positive than in the negative control.

KEY WORDS
beta-defensins; nasal mucosa; palatine tonsil; chronic inflammation

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