ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 9 (2011), No 1, p. 35-41
DOI 10.2478/v10136-009-00030-8

Inhibition of blood and tissue cholinesterases by soman in guinea pigs in vivo

Jiri Bajgar, Jiri Kassa, Miroslav Pohanka, Jana Zdarova Karasova, Ladislav Novotny, Josef Fusek, Vaclav Blaha

Address: Jiri Bajgar, Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
bajgar@pmfhk.cz

Received 28th April 2010.
Revised 18th May 2010.
Published online 4th November 2010.

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SUMMARY
Guinea pigs were intoxicated intramuscularly with different doses of soman, and cholinesterase activities were determined in the blood, diaphragm and parts of the brain - the pontomedullar area, the frontal cortex and the basal ganglia. The time course of poisoning following low doses (1,3, 5 microg/kg) and a dose equal to 1xLD50 (28.5 microg/kg) were studied. The dose having a negligible effect on cholinesterases in the tissues studied was assessed at 1-3 microg/kg, and, following administration of a dose of 5 microg/kg, statistically significant blood cholinesterase inhibition was demonstrated.

KEY WORDS
blood; brain parts; cholinesterases; guinea pig; soman; inhibition in vivo

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CITED

Pohanka M, Fusek J, Adam V, Kozek R. Carbofuran assay using Gelatin based Biosensor with Acetylcholinesterase as a Recognition Element. Int J Electrochem Sci. 8: 71-79, 2013.


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