ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 9 (2011), No 2, p. 103-109
DOI 10.2478/v10136-009-0032-6

Square wave voltammetry on screen printed electrodes: comparison to ferric reducing antioxidant power in plasma from model laboratory animal (Grey Partridge) and comparison to standard antioxidants

Miroslav Pohanka, Hana Bandouchova, Kristina Vlckova, Jana Zdarova Karasova, Kamil Kuca, Veronika Damkova, Lucie Peckova, Frantisek Vitula, Jiri Pikula

Address: Miroslav Pohanka, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
miroslav.pohanka@gmail.com

Received 24th May 2010.
Revised 1st July 2010.
Published online 23rd November 2010.

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SUMMARY
Low molecular weight antioxidants (LMWAs) were assayed by square wave voltammetry (SWV) using screen printed electrodes. Standard antioxidants, i.e. uric acid, ascorbic acid, trolox and glutathione, were assayed in order to estimate the sensitivity and standard redox potentials of individual LMWAs. In another experiment, plasma from Grey Partridges was used as model real samples. Ferric reducing antioxidant power (FRAP) was used as a reference method. Two peaks in plasma samples were found by SWV and correlated to FRAP. The SWV peaks were successfully correlated to FRAP. The practical importance of SWV carried out on screen printed electrodes is discussed.

KEY WORDS
electrochemistry; antioxidant assay; oxidative stress; reactive oxygen species; avian blood; biochemistry

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