ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 9 (2011) No 3, p 129-141
DOI 10.2478/v10136-011-0001-8

Effects of SDF-1alpha/CXCR4 on vascular smooth muscle cells and bone marrow mesenchymal cells in a rat carotid artery balloon injury model

Wen-Wei Cai, Ning-Yuan Fang, Jing Sheng, Shao-Jun Ma, Zhi-Hui Chen

Address: Ning-Yuan Fang, No. 639, Zhi-Zao-Ju Road, Shanghai 200011, P. R. China
funfeng111@gmail.com

Received 20th December 2010.
Revised 10th February 2011.
Published online 7th April 2011.

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SUMMARY
Bone marrow mensenchyme cells(BMSCs) can differentiate into endothelial progenitor cells which then migrate to injured sites for the repair of neointima, and stromal cell-derived factor-1alpha (SDF-1alpha) can mediate the migration of CXCR4 expressing stem/progenitor cells to injured sites for repair. Protein and mRNA expression of SDF-1alpha and CXCR4 were determined by RT-PCR, Western blot and ELISA. Immediately after common carotid artery balloon injury, the mRNA expression of SDF-1alpha in vascular smooth muscle cells(VSMCs) first increased and then decreased 7 days later. VSMCs transfected with SDF-1alpha siRNA did not express SDF-1alpha mRNA, but after transfection with SDF-1alpha siRNA, the SDF-1alpha content in injured VSMCs gradually returned to the baseline level. Normal BMSCs rarely expressed CXCR4 mRNA, but the CXCR4 mRNA expression on BMSCs increased significantly 4 days after common carotid artery injury and was maintained. The migration of BMSCs after artery injury was enhanced when compared with normal BMSCs, but SDF-1alpha siRNA transfection of VSMCs and AMD3100 treatment remarkably decreased the chemotaxis of BMSCs to VSMCs and SDF-1alpha, respectively. We conclude that the SDF-1alpha/CXCR4 axis plays an important role in the migration of BMSCs after balloon injury and can ultimately cause abnormal proliferation of the intima.

KEY WORDS
bone marrow mensenchyme cells; vascular smooth muscle cells; stromal cell-derived factor-1alpha

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