ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 9 (2011) No 3, p 151-156
DOI 10.2478/v10136-011-0002-7

Enhanced frequency of micronuclei in lymphocytes from current as opposed to former uranium miners

Friedo Zolzer, Zuzana Freitinger Skalicka, Renata Havrankova, Zdenek Hon, Leos Navratil, Jozef Rosina, Jiri Skopek

Address: Friedo Zolzer, University of South Bohemia, Faculty of Health and Social Studies, Department of Radiology and Toxicology and Department of Preclinical Studies, Ceske Budejovice, Czech Republic
zoelzer@zsf.jcu.cz

Received 21st January 2011.
Revised 22nd February 2011.
Published online 4th May 2011.

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SUMMARY
Micronuclei can be used as markers of past radiation exposure, but few pertinent studies have dealt with alpha radiation. Here we report on micronuclei in lymphocytes from uranium miners, comparing some that are currently active and others that retired 15-20 years ago. Their radiation exposure is assumed to come mainly from radon and its decay products in the air breathed at the work place.
Current miners showed a greater micronucleus frequency than former miners. This can be attributed to their recent radiation exposure, while the lower frequency in the former miners probably results from the disappearance of potentially micronucleus containing lymphocytes from the peripheral blood, which is known to occur with a half-life of about one year.
For current miners there is a significant correlation between micronucleus frequency and effective dose received over the last 12 months. The dose at which a doubling of the micronucleus frequency is observed is around 10 mSv. This is a much smaller dose than would usually be expected to be detectable with this test, and raises a number of questions about the induction of micronuclei by alpha radiation from radon and its decay products.

KEY WORDS
biodosimetry; micronucleus-centromere assay; alpha radiation; radon; uranium mining

REFERENCES
Abend M, Blakely WF, van Beuningen D. Simplified and optimized kinetochore detection: cytogenetic marker for late-G2 cells. Mutat Res 334:39-47, 1995.
[CrossRef]

Bejarano LA, Bolivar J, Valdivia MM. Anticentromere antibody specific to human cells directed against the CENP-B autoantigen. Cytogenet Cell Genet. 63: 54-58, 1993.
[CrossRef]

Bilbao A, Prosser JS, Edwards AA, Moody JC, Lloyd DC. The induction of micronuclei in human lymphocytes by in vitro irradiation with alpha particles from plutonium-239. Int J Radiat Biol. 56: 287-292, 1989.
[CrossRef]

Chang WP, Tsai M, Hwang J, Lin Y, Hsieh WA, Shao-yi H. Follow-up in the micronucleus frequencies and its subsets in human population with chronic low-dose gamma-irradiation exposure. Mutat Res. 428: 99-105, 1999.
[CrossRef]

Earnshaw WC, Sullivan KF, Machlin PS, Cooke CA, Kaiser DA, Pollard TD, Rothfield NF, Cleveland DW. Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen. J Cell Biol. 104: 817-829, 1987.
[CrossRef]

Fenech M, Morley AA. Measurement of micronuclei in lymphocytes. Mutat Res. 147: 29-36, 1985.

Hadjidekova VB, Bulanova M, Bonassi S, Neri M. Micronucleus frequency is increased in peripheral blood lymphocytes of nuclear power plant workers. Radiat Res. 160: 684-690, 2003.
[CrossRef]

Harley NH, Robbins ES. Radon and leukemia in the Danish study: another source of dose. Health Phys. 97: 343-347, 2009.
[CrossRef]

Johannes C, Dixius A, Pust M, Hentschel R, Buraczewska I, Staaf E, Brehwens K, Haghdoost S, Nievaart S, Czub J, Braziewicz J, Wojcik A. The yield of radiation-induced micronuclei in early and late-arising binucleated cells depends on radiation quality. Mutat Res. 701: 80-85, 2010.

Kribek B, Zak K, Dobes P, Leichmann J, Pudilova M, Rene M, Scharm B, Scharmova M, Hajek A, Holeczy D, Hein U, Lehmann B. The Rozna uranium deposit (Bohemian Massif, Czech Republic): shear zone-hosted, late Variscan and post-Variscan hydrothermal mineralization. Mineralium deposita. 44: 99-128, 2009.
[CrossRef]

Kryscio A, Ulrich Muller WU, Wojcik A, Kotschy N, Grobelny S, Streffer C. A cytogenetic analysis of the long-term effect of uranium mining on peripheral lymphocytes using the micronucleus-centromere assay. Int J Radiat Biol. 77: 1087-1093, 2001.
[CrossRef]

Little MP, Wakeford R, Kendall GM. Updated estimates of the proportion of childhood leukaemia incidence in Great Britain that may be caused by natural background ionising radiation. J Radiol Prot. 29: 467-482, 2009.
[CrossRef]

Michalek B. Mining of Radioactive Raw Materials as an Origin of the Nuclear Fuel Chain. Acta Montanistica Slovaca. 12: 115-125, 2007.

Muller WU, Streffer C. Micronucleus assays. In Obe G (ed.): Advances in Mutagenesis Research, Springer: Berlin, 1994, pp. 1–134.

Norppa H, Falck GCM. What do human micronuclei contain? Mutagenesis. 18: 221-233, 2003.
[CrossRef]

Norppa H, Renzi L, Lindholm C. Detection of whole chromosomes in micronuclei of cytokinesis-blocked human lymphocytes by antikinetochore staining and in situ hybridization. Mutagenesis. 8: 519-525, 1993.
[CrossRef]

Sari-Minodier I, Orsiere T, Bellon L, Pompili J, Sapin C, Botta A. Cytogenetic monitoring of industrial radiographers using the micronucleus assay. Mutat Res. 521: 37-46, 2002.

Sejkora J, Paulis P, Jelinek J, Vlk J. Nalezy cejkaitu v dulni chodbe uranoveho loziska Rozna, Ceska republika. Bulletin mineralogicko-petrografickeho oddeleni Narodniho muzea v Praze. 16: 212-216, 2008.

Thierens H, Vral A. The micronucleus assay in radiation accidents. Ann Ist Super Sanita. 45: 260-264, 2009.

Thierens H, Vral A, Barbe M, Aousalah B, De Ridder L. A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay. Mutat Res. 15: 105-111, 1999.

Thierens H, Vral A, Morthier R, Aousalah B, De Ridder L. Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay. Mutagenesis. 15: 245-249, 2000.
[CrossRef]

Thomson EJ, Perry PE. The identification of micronucleated chromosomes: a possible assay for aneuploidy. Mutagenesis. 3: 415-418, 1988.
[CrossRef]

Tomek P. Ceskoslovensky uran 1945-1989. Tezba a prodej ceskoslovenskeho uranu v ere komunismu. http://www.aplikace.mvcr.cz/archiv2008/policie/udv/sesity/sesit1/sesit1.doc, 2008 (accessed 11 February 2011).

Vral A, Thierens H, De Ridder L. In vitro micronucleus-centromere assay to detect radiation-damage induced by low doses in human lymphocytes. Int J Radiat Biol. 71: 61-68, 1997.
[CrossRef]

Wojcik A, Kowalska M, Bouzyk E, Buraczewska I, Kobialko G, Jarocewicz N, Szumiel I. Validation of the micronucleus-centromere assay for biological dosimetry. Genet Mol Biol. 23: 25–30, 2000.
[CrossRef]

World Nuclear Association. World Uranium Mining. http://www.world-nuclear.org/info/inf23.html, 2010 (accessed 11 February 2011).

Yamada Y, Oghiso Y, Enomoto H, Ishigure N. Induction of micronuclei in a rat alveolar epithelial cell line by alpha particle irradiation. Radiat Prot Dosim. 99: 219-222, 2002.

Zeni O, Chiavoni AS, Sannino A, Antolini A, Forigo D, Bersani F, Scarfi MR. Lack of genotoxic effects (micronucleus induction) in human lymphocytes exposed in vitro to 900 MHz electromagnetic fields. Radiat Res. 160: 152-158, 2003.
[CrossRef]
CITED

Zolzer F, Hon Z, Skalicka ZF, Havrankova R, Navratil L, Rosina J, Skopek J. Micronuclei in lymphocytes from currently active uranium miners. Radiat Environ Biophys. 51: 277-282, 2012.

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