ISSN 1214-0287 (on-line), ISSN 1214-021X (printed)
J Appl Biomed
Volume 9 (2011), No 4, p 231-241
DOI 10.2478/v10136-011-0012-5

Roles of fibrin deposition and protease activated receptor-1 in renal cytokine/chemokine production and inflammatory cell infiltration in rats of different ages

Shupeng Lin, Xuefeng Sun, Suozhu Shi, Chunsheng Xi, Quan Hong, Yang Lu, Xiangmei Chen

Address: Xiangmei Chen, State Key Lab of Kidney Diseases, Chinese PLA General Hospital & Military Medical Postgraduate College, 28 Fuxing Road, Beijing 100853, China
xmchen301@126.com

Received 8th May 2011.
Revised 14th June 2011.
Published online 7th July 2011.

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SUMMARY
The present study aimed to investigate the roles of fibrin deposition and protease activated receptor-1 (PAR-1) in renal cytokine/chemokine production and inflammatory cell infiltration in rats of different ages. Acute inflammation was induced by lipopolysaccharide (LPS) in rats which were then treated with tranexamic acid (TA), TA+urokinase (UK) or TA+low-molecular-weight heparin (HP). Fibrin deposition, inflammatory cells and expressions of PAR-1, monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule 1 (ICAM-1) were detected. A reduction in fibrin deposition and PAR-1 expression in the LPS+TA+HP group was associated with decreased infiltration of inflammatory cells and down-regulated expressions of MCP-1 and ICAM-1. In the LPS+TA+UK group, the fibrin deposition, but not the PAR-1 expression, was reduced, However, the infiltration of inflammatory cells decreased and the expressions of MCP-1 and ICAM-1 down-regulated. There were significant differences in the fibrin deposition, infiltration of inflammatory cells and expression of PAR-1, MCP-1 and ICAM-1 between young and old rats undergoing the same treatment. These findings demonstrated that fibrin deposition plays more important roles than PAR-1 dose in cytokine/chemokine production and inflammatory cell infiltration in vivo, and ageing may deteriorate the fibrin deposition-induced production of cytokines/chemokines and infiltration of inflammatory cells.

KEY WORDS
coagulation; fibrin deposition; protease activated receptor-1; ageing; kidney

REFERENCES
Aillaud MF, Pignol F, Alessi MC, Harle JR, Escande M, Mongin M, Juhan-Vague I. Increase in plasma concentration of plasminogen activator inhibitor, fibrinogen, von Willebrand factor, factor VIII: C and in erythrocyte sedimentation rate with age. Thromb Haemost. 55: 330-332, 1986.

Altieri DC. Regulation of leukocyte-endothelium interaction by fibrinogen. Thromb Haemost. 82: 781-786, 1999.

Balleisen L, Bailey J, Epping PH, Schulte H, van de Loo J. Epidemiological study on factor VII, factor VIII and fibrinogen in an industrial population. I. Baseline data on the relation to age, gender, bodyweight, smoking, alcohol, pill-using, and menopause. Thromb Haemost. 54: 475-479, 1985.

Berger J. Age-related sensitivity of rats to induction of anaemia. Fol Haematol. 114: 408-413, 1987a.

Berger J. Age-associated sensitivity to experimental drug-induced marrow hypoplasia in laboratory rats. Haematologia. 20: 171-178, 1987b.

Carthew P, Dorman BM, Edwards RE. Increased susceptibility of aged rats to haemorrhage and intravascular hypercoagulation following endotoxin administered in a generalized Shwartzman regime. J Comp Pathol. 105: 323-330, 1991.

Cirino G, Cicala C, Bucci MR, Sorrentino L, Maraganore JM, Stone SR. Thrombin functions as an inflammatory mediator through activation of its receptor. J Exp Med. 183: 821-827, 1996.

Cunningham MA, Rondeau E, Chen X, Coughlin SR, Holdsworth SR, Tipping PG. Protease-activated receptor 1 mediates thrombin-dependent, cell-mediated renal inflammation in crescentic glomerulonephritis. J Exp Med. 191: 455-462, 2000.

Cunningham MA, Kitching AR, Tipping PG, Holdsworth SR. Fibrin independent proinflammatory effects of tissue factor in experimental crescentic glomerulonephritis. Kidney Int. 66: 647-654, 2004.
[CrossRef]

Dang CV, Bell WR, Kaiser D, Wong A. Disorganization of cultured vascular endothelial cell monolayers by fibrinogen fragment D. Science. 227: 1487-1490, 1985.

Diamond MS, Springer TA. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol. 120: 545-556, 1993.

Furie B, Furie BC. The molecular basis of blood coagulation. Cell. 53: 505-518, 1988.

Grandaliano G, Valente AJ, Abboud HE. A novel biologic activity of thrombin: stimulation of monocyte chemotactic protein production. J Exp Med. 179: 1737-1741, 1994.
[CrossRef]

Hertig A, Rondeau E. Role of the coagulation/fibrinolysis system in fibrin-associated glomerular injury. J Am Soc Nephrol. 15: 844-853, 2004.

Horan MA, Pendleton N. The relationship between aging and disease. Clin Geron. 5: 125-141, 1995.

Languino LR, Plescia J, Duperray A, Brian AA, Plow EF, Geltosky JE, Altieri DC. Fibrinogen mediates leukocyte adhesion to vascular endothelium through an ICAM-1-dependent pathway. Cell. 73: 1423-1434, 1993.

Levi M, van der Poll T. Inflammation and coagulation. Crit Care Med. 38(Suppl. 2):S26-34, 2010.
[CrossRef]

Marty I, Peclat V, Kirdaite G, Salvi R, So A, Busso N. Amelioration of collagen-induced arthritis by thrombin inhibition. J Clin Invest. 107: 631-640, 2001.
[CrossRef]

Miller DL, Welty-Wolf K, Carraway MS, Ezban M, Ghio A, Suliman H, Piantadosi CA. Extrinsic coagulation blockade attenuates lung injury and proinflammatory cytokine release after intratracheal lipopolysaccharide. Am J Respir Cell Mol Biol. 26: 650-658, 2002.

Naruse T, Tsuchida A, Ogawa S, Yano S, Maekawa T. Selective glomerular thrombosis in rats induced by combined injections of nephrotoxic antiserum and lipopolysaccharide. J Lab Clin Med. 105: 146-156, 1985.

Neale TJ, Tipping PG, Carson SG, Holdsworth SR. Participation of cell-mediated immunity in deposition of fibrin in glomerulonephritis. Lancet. 2: 421-424, 1988.

Nham SU. Characteristics of fibrinogen binding to the domain of CD11c, an alpha subunit of p150,95. Biochem Biophys Res Commun. 264: 630-634, 1999.
[CrossRef]

Perez RL, Roman J. Fibrin enhances the expression of IL-1beta by human peripheral blood mononuclear cells: implications in pulmonary inflammation. J Immunol. 154: 1879-1887, 1995.

Shankar R, de la Motte CA, Poptic EJ, DiCorleto PE. Thrombin receptor-activating peptides differentially stimulate platelet-derived growth factor production, monocytic cell adhesion, and E-selectin expression in human umbilical vein endothelial cells. J Biol Chem. 269: 13936-13941, 1994.

Szaba FM, Smiley ST. Roles for thrombin and fibrin(ogen) in cytokine/chemokine production and macrophage adhesion in vivo. Blood. 99: 1053-1059, 2002.
[CrossRef]

Ueno A, Murakami K, Yamanouchi K, Watanabe M, Kondo T. Thrombin stimulates production of interleukin-8 in human umbilical vein endothelial cells. Immunology. 88: 76-81, 1996.

Varisco PA, Peclat V, van Ness K, Bischof-Delaloye A, So A, Busso N. Effect of thrombin inhibition on synovial inflammation in antigen induced arthritis. Ann Rheum Dis. 59: 781-787, 2000.

Wada T, Yokoyama H, Matsushima K, Kobayashi K. Chemokines in renal diseases. Int Immunopharmacol. 1: 637-645, 2001.

Welty-Wolf KE, Carraway MS, Miller DL, Ortel TL, Ezban M, Ghio AJ, Idell S, Piantadosi CA. Coagulation blockade prevents sepsis induced respiratory and renal failure in baboons. Am J Respir Crit Care Med. 164: 1988-1996, 2001.

Yamamoto K, Shimokawa T, Yi H, Isobe K, Kojima T, Loskutoff DJ, Saito H. Aging accelerates endotoxin -induced thrombosis increased responses of plasminogen activator inhibitor-1 and lipopolysaccharide signaling with aging. Am J Pathol. 161: 1805-1814, 2002.
CITED

Gutowska I, Baranowska-Bosiacka I, Safranow K, Jakubowska K, Olszewska M, Telesinski A, Siennicka A, Drozdzik M, Chlubek D, Stachowska E. Fluoride in low concentration modifies expression and activity of 15 lipoxygenase in human PBMC differentiated monocyte/macrophage. Toxicol. 295: 23-30, 2012.

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