J Appl Biomed 18:106-114, 2020 | DOI: 10.32725/jab.2020.014

Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

Daliah Alves Coelho Trevisan1,*, Paula Aline Zanetti Campanerut-Sá1, Alex Fiori da Silva2, Andreia Farias Pereira Batista1, Flavio Augusto Vicente Seixas3, Rosane Marina Peralta4, Anacharis Babeto de Sá-Nakanishi4, Benício Alves de Abreu Filho5, Miguel Machinski Junior5, Jane Martha Graton Mikcha1
1 State University of Maringá, Department of Clinical Analysis and Biomedicine, Maringá, Brazil
2 State University of Minas Gerais, Department of Biological Sciences, Ituiutaba, Brasil
3 State University of Maringá, Department of Technology, Umuarama, Brazil
4 State University of Maringá , Department of Biochemistry, Maringá, Brazil
5 State University of Maringá, Department of Basic Health Sciences, Maringá, Brazil

Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.

Keywords: Carvacrol; Biofilms; Proteome; Salmonella Typhimurium
Grants and funding:

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001 and the Complexo de Centrais de Apoio à Pesquisa (COMCAP) of the State University of Maringá. We also thank the Laboratory of Mass Spectrometry at the Brazilian Biosciences National Laboratory (LNBio), CNPEM, CampiTrevisan et al. / J Appl Biomed 113 nas, Brazil for their support with the use of RP-nano UPLC (nanoAcquity, Waters) equipment coupled with a Q-Tof Premier mass spectrometer (Waters).

Conflicts of interest:

The authors declare that they have no conflict of interests.

Received: April 9, 2020; Revised: July 30, 2020; Accepted: September 8, 2020; Prepublished online: September 22, 2020; Published: December 14, 2020  Show citation

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Alves Coelho Trevisan D, Aline Zanetti Campanerut-Sá P, da Silva AF, Farias Pereira Batista A, Seixas FAV, Peralta RM, et al.. Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study. J Appl Biomed. 2020;18(4):106-114. doi: 10.32725/jab.2020.014. PubMed PMID: 34907763.
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