J Appl Biomed 19:149-158, 2021 | DOI: 10.32725/jab.2021.017

Effect of the natural flavonoids myricetin and dihydromyricetin on the wound healing process in vitro

Renáta Sklenářová1, Marika Svrčková1, Petr Hodek2, Jitka Ulrichová1, Jana Franková1, *
1 Palacký University Olomouc, Faculty of Medicine and Dentistry, Department of Medical Chemistry and Biochemistry, Olomouc, Czech Republic
2 Charles University, Faculty of Science, Department of Biochemistry, Prague 2, Czech Republic

Myricetin (MYR) and dihydromyricetin (DHM) are classified as natural flavonoids. Both substances are known for their anti-inflammatory and antioxidant properties. In this study, an in vitro model of inflammation was demonstrated on monolayers of scratched fibroblasts or keratinocytes exposed to LPS from Pseudomonas aeruginosa for six hours. MYR and DHM were subsequently applied to the cells for 24 hours at sub toxic concentrations (5-15 µM). Inflammatory parameters were analysed in collected cell medium and lysate after the incubation period using the Enzyme-Linked ImmuneSorbent Assay (ELISA) and Western blot. Both flavonoids inhibit the production of pro-inflammatory cytokines (IL-6, IL-8) in LPS-stimulated skin cells as well as the decreased level of MMP-1 in fibroblasts. However, the application of MYR and DHM dose dependently increased the level of MMP-1 in keratinocytes. In our experiments, we focused on the anti-glycation activity of MYR and DHM, where the higher concentration of MYR seems to be more effective.

Keywords: Dihydromyricetin; Inflammation; Myricetin; Skin wound healing
Grants and funding:

This work was supported by the grants IGA_LF_2021_011, IGA_LF_2019_015 and P303/12/G163 from the Czech Science Foundation (GACR).

Conflicts of interest:

The authors declare no conflict of interests.

Received: November 18, 2020; Revised: April 6, 2021; Accepted: July 20, 2021; Prepublished online: August 10, 2021; Published: September 17, 2021  Show citation

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Sklenářová R, Svrčková M, Hodek P, Ulrichová J, Franková J. Effect of the natural flavonoids myricetin and dihydromyricetin on the wound healing process in vitro. J Appl Biomed. 2021;19(3):149-158. doi: 10.32725/jab.2021.017. PubMed PMID: 34907758.
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