Journal of Applied Biomedicine, 2023 (vol. 21), issue 1
Original Research Article
Closed reduction and minimally invasive screw osteosynthesis of Pipkin femoral head fractures
Martin Kloub *, Karel Holub, Marek Peml, Jiøí Urban, Pavel Látal
J Appl Biomed 21:1-6, 2023 | DOI: 10.32725/jab.2023.001 
Background: Femoral posterior hip dislocation with associated femoral head fractures (Pipkin fractures) are rare high-energy injuries. Published treatment modalities involve conservative treatment, head fragment resection, open reduction and internal fixation, and total hip replacement. The experience with mini-invasive screw osteosynthesis of these fractures is the main focus of our study. Methods: Seven Pipkin fractures (five Pipkin II and two Pipkin I) in six patients were treated by closed reduction of hip dislocation, followed by minimal invasive lag screw osteosynthesis. Cancellous screw(s) were inserted from the incision on the lateral hip through...
Comparison of cardiocerebrovascular disease incidence between angiotensin converting enzyme inhibitor and angiotensin receptor blocker users in a real-world cohort
Suehyun Lee, Hyunah Kim, Hyeon Woo Yim, Hun-Sung Kim, Ju Han Kim
J Appl Biomed 21:7-14, 2023 | DOI: 10.32725/jab.2023.002
Background: Both angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin II receptor blockers (ARBs) are known to be effective in managing cardiovascular diseases, but more evidence supports the use of an ACEI. This study investigated the difference in cardiovascular disease incidence between relatively low-compliance ACEIs and high-compliance ARBs in the clinical setting. Methods: Patients who were first prescribed ACEIs or ARBs at two tertiary university hospitals in Korea were observed in this retrospective cohort study for the incidence of heart failure, angina, acute myocardial infarction, cerebrovascular disease, ischemic heart disease,...
hKLK alleviates myocardial fibrosis in mice with viral myocarditis
Youfa Qin, Xiaomei Ye, Ye Luo, Luting Peng, Guanghui Zhou, Yongkun Zhu, Chunyu Pan
J Appl Biomed 21:15-22, 2023 | DOI: 10.32725/jab.2023.005
Myocardial fibrosis is the most serious complication of viral myocarditis (VMC). This study aimed to investigate the therapeutic benefits and underlying mechanisms of lentivirus-mediated human tissue kallikrein gene transfer in myocardial fibrosis in VMC mice. We established VMC mouse model via intraperitoneal injection with Coxsackie B3 virus. The effect was then assessed after treatment with vehicle, the empty lentiviral vectors (EZ.null), and the vectors expressing hKLK1 (EZ.hKLK1) via tail vein injection for 30 days, respectively. The results showed that administering EZ.hKLK1 successfully induced hKLK1 overexpression in mouse heart. Compared with...
As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy
Ruoxi Zhao, Shigang Zheng, Ying Li, Xueqin Zhang, Dan Rao, Ze Chun, Yadong Hu
J Appl Biomed 21:23-35, 2023 | DOI: 10.32725/jab.2022.019
Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb. However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored. In this study, the differences between ether extract and ethanol extract of Dendrobium nobile Lindl. on chemical components and anticancer activities were investigated, as well as the anticancer mechanisms among different extracts. The results demonstrated that the ether extract exhibited a stronger anticancer effect...
Amaryllidaceae alkaloids in skin cancer management: Photoprotective effect on human keratinocytes and anti-proliferative activity in melanoma cells
Carol Castañeda, Karent Bravo, Natalie Cortés, Janeth Bedoya, Warley de S. Borges, Jaume Bastida, Edison Osorio
J Appl Biomed 21:36-47, 2023 | DOI: 10.32725/jab.2023.004
Skin cancer has high rates of mortality and therapeutic failure. In this study, to develop a multi-agent strategy for skin cancer management, the selective cytotoxicity of several alkaloid fractions and pure alkaloids isolated from Amaryllidaceae species was evaluated in melanoma cells. In addition, UVB-stimulated keratinocytes (HaCaT) were exposed to seven alkaloid fractions characterized by GC-MS, and the production of intracellular reactive oxygen species (ROS) and IL-6, were measured to evaluate their photoprotection effects. The Eucharis caucana (bulb) alkaloid fraction (20 μg/ml) had a clear effect on the viability of melanoma cells,...
Effects of oleanolic acid on hair growth in mouse dorsal skin mediated via regulation of inflammatory cytokines
Bing Zhang, Wenyun Zhang, Jianfang Luo, Jian He, Xiaomin Zheng, Siyang Zhu, Baoshan Rong, Yong Ai, Lanyue Zhang, Tinggang He
J Appl Biomed 21:48-57, 2023 | DOI: 10.32725/jab.2023.003
Oleanolic acid (OA) is a pentacyclic triterpenoid with favourable physiological activity. It is widely distributed in more than 200 species of plants. OA has garnered significant interest because of its potential biological activities, such as antioxidant, bacteriostatic, and hair growth-promoting effects. To study the effect of OA on hair growth and related mechanisms, we investigated hair growth in mice with testosterone-induced androgenetic alopecia (AGA) that were treated with three different concentrations of OA. The antioxidant, bacteriostatic, and cytotoxic effects of OA were evaluated. We found that mice with testosterone-induced AGA...