Journal of Applied Biomedicine, 2023 (vol. 21), issue 2
Original Research Article
Sequential hybrid ablation versus surgical CryoMaze alone for treatment of atrial fibrillation (SurHyb Trial): a protocol of the multicentre randomized controlled trial
Alan Bulava, Aleš Mokráček, Dan Wichterle, Petr Budera, Pavel Osmančík, Petr Kačer, Linda Vetešková, Petr Němec, Tomáš Skála, Petr Šantavý, Jan Chovančík, Piotr Branny, Vitalii Rizov, Miroslav Kolesár, Marian Rybář
J Appl Biomed 21:67-72, 2023 | DOI: 10.32725/jab.2023.007 
Background: Atrial fibrillation is common in patients with structural heart disease who are undergoing cardiac surgery. Surgical CryoMaze has been shown to be an effective treatment in several trials, but success rates have varied considerably, between 47-95%. The sequential hybrid approach, combining surgical CryoMaze followed by radiofrequency catheter ablation, can achieve high freedom from atrial arrhythmias. However, in patients with concomitant surgical atrial fibrillation treatment, data comparing the hybrid approach to CryoMaze alone are lacking. Methods: The SurHyb study was designed as a prospective, open-label, multicentre randomized trial....
In vitro study of Nigella sativa and thymoquinone activity on endothelial activation and monocyte adhesion
Al'Aina Yuhainis Firus Khan, Fahmi Mohtar, Thuhairah Abdul Rahman, Suhaila Abdul Muid, Gabriele Ruth Anisah Froemming, Hapizah Nawawi
J Appl Biomed 21:73-79, 2023 | DOI: 10.32725/jab.2023.006
Introduction: Thymoquinone (TQ) is one of the bioactive compounds in Nigella sativa (NS). Also known as black seeds/cumin, it has been postulated to possess anti-atherogenic properties. However, research on the effects of NS oil (NSO) and TQ on atherogenesis remain scarce. The aim of this study is to determine gene and protein expression of Intercellular Adhesion Molecule-1 (ICAM-1), Vascular Cell Adhesion Molecule-1 (VCAM-1), and Endothelial-eukocyte adhesion molecule (E-selectin) in Human Coronary Artery Endothelial Cells (HCAECs). Methods: HCAECs were stimulated for 24 hours (h) with 200 µg/ml of Lipopolysaccharides (LPS) and different...
Antioxidant action of xanthine oxidase inhibitor febuxostat protects the liver and blood vasculature in SHRSP5/Dmcr rats
Mai Kakimoto, Moe Fujii, Ikumi Sato, Koki Honma, Hinako Nakayama, Sora Kirihara, Taketo Fukuoka, Shang Ran, Satoshi Hirohata, Kazuya Kitamori, Shusei Yamamoto, Shogo Watanabe
J Appl Biomed 21:80-90, 2023 | DOI: 10.32725/jab.2023.009
Background: Xanthine oxidase (XO) generates reactive oxygen species during uric acid production. Therefore, XO inhibitors, which suppress oxidative stress, may effectively treat non-alcoholic steatohepatitis (NASH) and atherosclerosis via uric acid reduction. In this study, we examined the antioxidant effect of the XO inhibitor febuxostat on NASH and atherosclerosis in stroke-prone spontaneously hypertensive 5 (SHRSP5/Dmcr) rats. Methods: SHRSP5/Dmcr rats were divided into three groups: SHRSP5/Dmcr + high-fat and high-cholesterol (HFC) diet [control group, n = 5], SHRSP5/Dmcr + HFC diet + 10% fructose (40 ml/day) [fructose group, n =...
The hepatoprotective effect of Sophora viciifolia fruit extract against acetaminophen-induced liver injury in mice
Shanshan Qi, Beibei Lin, Sanqiao Wu, Hao Hao, Hongxin Zheng, Xiang Liu, Xiaoying Zhang, Lijuan Yue, Chen Chen
J Appl Biomed 21:91-98, 2023 | DOI: 10.32725/jab.2023.008
This research demonstrated the protective effect and possible mechanism of the Sophora viciifolia extract (SVE) against acetaminophen-induced liver injury in mice. The levels of ALT and AST in the serum and antioxidant enzyme activity in the liver were measured. We used immunohistochemistry to detect CYP2E1, Nrf2, and Keap1 protein expression in the liver. The mRNA expression in the liver of TNF-α, NF-κB, and IL-6, Nrf2 and its downstream genes HO-1 and GCLC were measured by qRT-PCR. We found that SVE could decrease the ALT and AST levels, promote the activities of SOD, CAT, GSH-Px,...
Experimental study on clinical and morphological determination of the optimal cannula diameter for lipoaspirate harvest from rabbit inguinal fat pad
Ilona Pak, Meirambek Askarov, Nurlan Kissamedenov, Dmitriy Klyuyev, Yevgeniy Kamyshanskiy
J Appl Biomed 21:99-105, 2023 | DOI: 10.32725/jab.2023.011
Determining the most appropriate cannula diameter for lipoaspirate harvesting is important, both in terms of the quality and composition of the material obtained, and the ease of practical use of the cannula. The size of the cannula is one of the main factors affecting the qualitative characteristics of the obtained lipoaspirate sample for further use of adipose tissue. The purpose of the investigation was to clinically and histomorphometrically determine the optimal cannula diameter for lipoaspirate samples collection from rabbit inguinal fat pad in an experimental study. The methods of Animal models, Surgical procedures, Macroscopic examination,...
Review Article
Causality assessment of adverse drug reaction: A narrative review to find the most exhaustive and easy-to-use tool in post-authorization settings
Pallavi Pradhan, Maude Lavallée, Samuel Akinola, Fernanda Raphael Escobar Gimenes, Anick Bérard, Julie Méthot, Marie-Eve Piché, Jennifer Midiani Gonella, Lyne Cloutier, Jacinthe Leclerc
J Appl Biomed 21:59-66, 2023 | DOI: 10.32725/jab.2023.010
Background: The core motive of pharmacovigilance is the detection and prevention of adverse drug reactions (ADRs), to improve the risk-benefit balance of the drug. However, the causality assessment of ADRs remains a major challenge among clinicians, and none of the available tools of causality assessment used for assessing ADRs have been universally accepted. Objective: To provide an up-to-date overview of the different causality assessment tools. Methods: We conducted electronic searches in MEDLINE, EMBASE, and the Cochrane database. The eligibility of each tool was screened by three reviewers. Each eligible tool was then scrutinized for its domains...