J Appl Biomed 8:41-46, 2010 | DOI: 10.2478/v10136-009-0006-8

Pesticide sorption in typical Central European soils evaluated using a photometric microplate assay based on acetylcholinesterase inhibition

Miroslav Pohanka1,*, Vítězslav Vlček2, Kamil Kuča1, Hana Bandouchová3, Jiří Pikula3
1 Center of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
2 Department of Agrochemistry, Soil Science, Microbiology and Plant Nutrition, Mendel University of Agriculture and Forestry Brno, Czech Republic
3 Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic

Sorption isotherms were estimated for two model organophosphorus pesticides - methamidophos and paraoxon-ethyl - and four typical Central European soils endangered by these pesticides: haplic Chernozems, Cambisols, Luvisols, and Fluvisols. A photometric microplate assay based on the recognition capability of the enzyme acetylcholinesterase toward organophosphorous pesticides was used for the construction of sorption isotherms. The sorption capacity of each soil was then determined and it was found that the soils with the highest content of humic acid (haplic Chernozems and haplic Luvisols) sorbed pesticides most. Pesticides in concentrations over the sorption capacity were easily removed from soil by water.

Keywords: organophosphates; methamidophos; paraoxon; sorption isotherms; capacity

Received: July 16, 2009; Revised: September 11, 2009; Published: March 31, 2010  Show citation

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Pohanka M, Vlček V, Kuča K, Bandouchová H, Pikula J. Pesticide sorption in typical Central European soils evaluated using a photometric microplate assay based on acetylcholinesterase inhibition. J Appl Biomed. 2010;8(1):41-46. doi: 10.2478/v10136-009-0006-8.
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