J Appl Biomed 13:7-18, 2015 | DOI: 10.1016/j.jab.2014.01.004

HPLC analysis and antimicrobial, antimycobacterial and antiviral activities of Tabernaemontana catharinensis A. DC

Aline Augusti Boligona,*, Mariana Pianaa, Thaís Felli Kubiçab, Débora Nunes Marioc, Tanise Vendruscolo Dalmolind, Pauline Cordenonsi Bonezd, Rudi Weiblenb, Luciane Lovatob, Sydney Hartz Alvesc, Marli M.A. Camposd, Margareth Linde Athaydea
a Phytochemical Laboratory, Department of Pharmacy Industrial, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil
b Virology Laboratory, Department of Veterinary Preventive Medicine, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil
c Mycological Research Laboratory, Department of Microbiology and Parasitology, Federal University of Santa Maria (UFSM), 97105-900 Santa Maria, RS, Brazil
d Mycobacterial Research Laboratory, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil

Antimicrobial, antimycobacterial and antiviral activities of crude extracts, fractions and subfractions of Tabernaemontana catharinensis were evaluated. Best antimicrobial results occurred with the dichloromethane (DCM) and butanolic (NB) fractions against Staphylococcus aureus, Micrococcus sp., Enterococcus faecalis and Bacillus subtilis (minimal inhibitory concentration, MIC = 31.25-1000 μg/mL). Considering the Gram-negative bacteria, only NB fraction was effective against Proteus mirabilis and Aeromonas sp. (MIC = 62.5 μg/mL and 250 μg/mL, respectively). In addition, the fungi Candida albicans, Candida glabrata, Cryptococcus neoformans, Saccharomyces cerevisiae, Aspergillus flavus and Aspergillus fumigatus were particularly vulnerable for DCM fraction (MIC = 31.25-1000 μg/mL). The fractions and subfractions were effective against Mycobacterium smegmatis (MIC = 19.53-156.25 μg/mL). DCM (selectivity index - SI = 77.92), ethyl acetate (EA) (SI = 40.27) and NB (SI = 28.97) fractions from the leaves exhibited a potential antiviral activity toward Herpes Simplex Virus type 1 whereas DCM2 subfraction from leaves (SI = 12.28) and alkaloidal fraction (AF) (10.71) maintained this good activity. Steroids, terpenoids and phenolics compounds were identified by high-performance liquid chromatography with diode array detection (HPLC/DAD) and may be partially responsible for the antimicrobial and antiherpes activities observed. The results obtained in this study showed that T. catharinensis has antimicrobial and anti-herpetic activities and that these properties are reported for the first time for this species.

Keywords: Apocynaceae; Cobrina; HSV-1; HPLC; Mycobacterium

Received: November 29, 2013; Revised: January 15, 2014; Accepted: January 20, 2014; Published: February 1, 2015  Show citation

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Boligon AA, Piana M, Felli Kubiça T, Nunes Mario D, Dalmolin TV, Bonez PC, et al.. HPLC analysis and antimicrobial, antimycobacterial and antiviral activities of Tabernaemontana catharinensis A. DC. J Appl Biomed. 2015;13(1):7-18. doi: 10.1016/j.jab.2014.01.004.
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