J Appl Biomed 13:19-22, 2015 | DOI: 10.1016/j.jab.2014.04.003

Chicken immunoglobulins for prophylaxis: Effect of inhaled antibodies on inflammatory parameters in rat airways

Jana Hadrabová^a, Barbora Majerová^a, Věra Černá^a, Michaela Moserová^a, Radim Holuša^b, Václav Mandys^c, Marie Stiborová^a, Ilja Stříž^d, Petr Hodek^a,*

^a Charles University in Prague, Faculty of Science, Department of Biochemistry, Hlavova 2030, CZ-128 40 Prague 2, Czech Republic

^b National Institute of Public Health, Prague 10, Czech Republic

^c Charles University in Prague, 3rd Faculty of Medicine, Department of Pathology, Prague 10, Czech Republic

^d Institute for Clinical and Experimental Medicine, Prague 4, Czech Republic

The prophylaxis against microbial airway infections of cystic fibrosis (CF) patients is an emerging application of chicken yolk antibody (IgY), however, no data on the effect of inhaled IgY have been published yet. Rats were daily (for 28 days) exposed to an aerosol of IgY, ovalbumin (OVA), Fab fragment of IgY, or PBS and their serum, bronchoalveolar lavage (BAL) and lung tissue were examined for inflammation signs. There were no marked changes in lung parenchyma, except for an elevated number of alveolar macrophages in the OVA-exposed group. While the administration of OVA or IgY aerosols slightly increased levels of cytokine TNF-α and GRO/KC in BAL fluid, a marked elevation of GM-CSF in serum was observed after the OVA inhalation. The administration of Fab induced expression of IL-1β > IL-18 in serum, in contrast no effect exerted by IgY. Our results suggest that the aerosolized IgY did not cause any deleterious effects in rat lungs.

Keywords: Yolk antibody; Cystic fibrosis; Inhalation prophylaxis; Bronchoalveolar lavage; Cytokine; Inflammation

Received: March 1, 2014; Accepted: April 16, 2014; Published: February 1, 2015  Show citation