J Appl Biomed 19:97-104, 2021 | DOI: 10.32725/jab.2021.010

Astragalus polysaccharide alleviates alveolar bone destruction by regulating local osteoclastogenesis during periodontitis

Yakun Han *, Chengcheng Yu, Yan Yu
Affiliated Hospital of Jilin Medical University, Department of Stomatology, Jilin, China

Inflammatory imbalance of bone formation/resorption leads to alveolar bone destruction. Astragalus polysaccharide has been confirmed to have anti-inflammatory effects. We sought to disclose the protective effect and its potential mechanisms of astragalus polysaccharide in the periodontitis model. Experimental periodontitis was induced by cotton ligatures for this study. We measured the alveolar bone damage rate, periodontal osteoclasts, proportion of CD4+Foxp3+, CD4+IL-10+, CD4+TGF-β+ subsets in the gingiva, and RANKL, OPG, TGF-β+, and IL-10+ level in the gingiva. We also cultured osteoclast precursor cells in the presence of RANKL and astragalus polysaccharide. Osteoclasto-like cells were identified by TRAP staining, mRNA of RANK, TRAP, and TRAF6 were evaluated by real time PCR. We found that astragalus polysaccharide caused significant protection of the alveolar bone via reducing local osteoclasts. It also decreased the proportion of CD4+Foxp3+ cells and upregulated the level of CD4+IL-10+ cells, reduced RANKL, and remedied IL-10 levels. In cell culture experiments, astragalus polysaccharide prohibited the RANKL mediated osteoclast differentiation. The findings of this study disclose the functions and possible mechanisms of astragalus polysaccharide engaged in local osteoclastogenesis, and reveal the considerable effect of astragalus polysaccharide in alveolar bone homeostasis and its likely contribution to host immuno-regulation in periodontitis.

Keywords: Astragalus polysaccharide; Osteoclastogenesis; Periodontitis; RANKL; Tregs
Grants and funding:

This work was supported by grants from Science and Technology Plan of Chinese Traditional Medicine (2018127), Province Jilin, China.

Conflicts of interest:

The authors have no conflict of interests to declare.

Received: July 25, 2020; Revised: January 23, 2021; Accepted: February 23, 2021; Prepublished online: April 15, 2021; Published: May 10, 2021  Show citation

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Han Y, Yu C, Yu Y. Astragalus polysaccharide alleviates alveolar bone destruction by regulating local osteoclastogenesis during periodontitis. J Appl Biomed. 2021;19(2):97-104. doi: 10.32725/jab.2021.010. PubMed PMID: 34907709.
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