J Appl Biomed 22:99-106, 2024 | DOI: 10.32725/jab.2024.008
Resveratrol alleviates endoplasmic reticulum stress-induced cell death and improves functional prognosis after traumatic brain injury in mice
- 1 The First Affiliated Hospital of Ningbo University, Department of Neurology, Ningbo 315000, China
- 2 Ningbo Medical Center Lihuili Hospital, Department of Rehabilitation, Ningbo 315000, China
Resveratrol (RSV) is a polyphenol antioxidant that has been shown to have neuroprotective effects. We sought molecular mechanisms that emphasize the anti-inflammatory activity of RSV in traumatic brain injury (TBI) in mice associated with endoplasmic reticulum stress (ERS). After establishing three experimental groups (sham, TBI, and TBI+RSV), we explored the results of RSV after TBI on ERS and caspase-12 apoptotic pathways. The expression levels of C/EBP homologous protein (CHOP), glucose regulated protein 78kD (GRP78), caspase-3, and caspase-12 in cortical brain tissues were assessed by western blotting. The qPCR analysis was also performed on mRNA expression of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in cortical brain tissue. In addition, the expression of GRP78 in microglia (ionized calcium binding adaptor molecule 1; Iba-1) and neurons (neuronal nuclei; NeuN) was identified by immunofluorescence staining. The neurological function of mice was assessed by modified neurological severity scores (mNSS). After drug treatment, the expression of CHOP, GRP78, caspase-3 and caspase-12 decreased, and qPCR results showed that TNF-α and IL-1β were down-regulated. Immunofluorescence staining showed down-regulation of Iba-1+/GRP78+ and NeuN+/GRP78+ cells after RSV treatment. The mNSS analysis confirmed improvement after RSV treatment. RSV improved apoptosis by downregulating the ERS signaling pathway and improved neurological prognosis in mice with TBI.
Keywords: Apoptosis; Endoplasmic reticulum stress; Resveratrol; Traumatic brain injury
Grants and funding:
The study was supported by Ningbo Health Technology Project, No: 2020Y12 and 2022Y12.
Conflicts of interest:
The authors have no conflict of interest to declare.
Received: June 16, 2023; Revised: February 19, 2024; Accepted: March 27, 2024; Prepublished online: April 17, 2024; Published: June 24, 2024 Show citation
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