J Appl Biomed 22:107-114, 2024 | DOI: 10.32725/jab.2024.014

Ethyl pyruvate attenuates cellular adhesion and proliferation of diffuse large B-cell lymphoma by targeting c-Jun

Zhimin Yan1 a, Qiong Zhong2 a, Ling Yan3, Wenhong Lai1, Xi Xu1 *
1 The First Affiliated Hospital of Gannan Medical University, Department of Hematology, Ganzhou, 341000, China
2 The First Affiliated Hospital of Gannan Medical University, Department of Pain, Ganzhou, 341000, China
3 The First Affiliated Hospital of Gannan Medical University, Department of Ultrasonography, Ganzhou, 341000, China

Diffuse large B-cell lymphoma (DLBCL) stands out as the most common type of malignant cancer, representing the majority of cases of non-Hodgkin's lymphoma. Ethyl pyruvate (EP) is a derivative of pyruvic acid and found to have potent anti-tumor properties. Despite its potential benefits, the impact of EP on DLBCL remains ambiguous. Our objective is to elucidate the role of EP in modulating the development of DLBCL. Analysis of cholecystokinin-8 (CCK-8) revealed that treatment with EP significantly diminished the viability of DLBCL cells. Furthermore, EP administration suppressed colony formation and hindered cell adhesion and invasion in DLBCL cells. Examination of cell cycle progression showed that EP treatment induced arrest at the G1 phase and subsequently reduced the S phase population in DLBCL cells. EP treatment consistently exhibited apoptosis-inducing properties in Annexin-V assays, and notably downregulated the expression of Bcl-2 while increasing levels of proapoptotic cleaved caspase 3 and BAX in DLBCL cells. Additionally, EP treatment decreased the overexpression of c-Jun in c-Jun-transfected DLBCL cells. Further, EP demonstrated DNA-damaging effects in TUNEL assays. In vivo, xenograft animal models revealed that EP treatment significantly mitigated DLBCL tumor growth and suppressed DLBCL cell adhesion to bone marrow stromal cells. In summary, these findings suggest that EP mitigates DLBCL progression by inducing apoptosis, inducing cell cycle arrest, and promoting DNA damage.

Keywords: Adhesion; c-Jun; Diffuse large B cell lymphoma; Ethyl pyruvate; Proliferation
Grants and funding:

This work was supported in part by funding from the Science and Technology Program of Jiangxi Provincial Health Commission (SKJP520201086-202130697).

Conflicts of interest:

The authors have no conflict of interest to declare.

Received: July 24, 2023; Revised: February 22, 2024; Accepted: June 20, 2024; Prepublished online: June 20, 2024; Published: June 24, 2024  Show citation

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Yan Z, Zhong Q, Yan L, Lai W, Xu X. Ethyl pyruvate attenuates cellular adhesion and proliferation of diffuse large B-cell lymphoma by targeting c-Jun. J Appl Biomed. 2024;22(2):107-114. doi: 10.32725/jab.2024.014. PubMed PMID: 38912866.
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