Journal of Applied Biomedicine, 2005 (vol. 3), issue 4

Editorial

To the cell and molecular biology, cell physiology and pathology, cell morphology, structure and cytochemistry - the struggle for publication

Karel Smetana

J Appl Biomed 3:155-157, 2005 | DOI: 10.32725/jab.2005.020

The editorial boards with responsible reviewers in scientific journals might play a very important role and should be of a great help for authors. It is a reasonable hope that the journals with such reviewers might be very much respected as well as and highly evaluated without overestimation of the impact factor.

Original Research Article

Expression and translocation of major nucleolar proteins in relation to the transcriptional activity of the nucleolus

Ivan Kalousek, Petra Otevřelová, Pavla Röselová

J Appl Biomed 3:175-186, 2005 | DOI: 10.32725/jab.2005.023

Changes in the expression and cellular localization of abundant nucleolar proteins: C23/nucleolin, upstream binding factor (UBF), B23/nucleophosmin, and fibrillarin were examined in human lymphocytes subjected to the control of nucleolar activity by phytohemagglutinin and actinomycin D. Data suggest that the up-regulation of ribosomal RNA transcription induced by phytohemagglutinin was accompanied by a significant increase in the nucleolar content of C23/nucleolin, UBF, B23/nucleophosmin, whilst the nucleolar content of fibrillarin had a relatively low-variable. An unraveling of the multicopy ribosomal gene accompanying the mitogenic stimulation was...

The chemopreventive effect of diallyl disulphide on N-nitrosodiethylamine induced heptocarcinogenesis

Thamilarasan Manivasagam, Perumal Subramanian, Ganapathy Suthakar, Musthafa Mohamed Essa

J Appl Biomed 3:187-191, 2005 | DOI: 10.32725/jab.2005.024

In the present study we have investigated the anticarcinogenic property of diallyl disulphide (DADS) on hepatic thiobarbituric acid reactive substances (TBARS) and antioxidants such as reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase in control, N-nitrosodiethylamine and diallyl disulphide treated rats. The levels of TBARS and activities of SOD and catalase were decreased in NDEA treated rats whereas GSH and GPx tended to increase in carcinogenic rats. Oral administration of DADS (60mg/kg body wt) tends to normalize these variables in liver. This clearly indicates that DADS could possess chemopreventive...

A morphological and cytochemical note to the reversible intranucleolar translocation of AgNORs (silver stained nucleolus organizer regions) in early leukemic granulocytic progenitors represented by cultured K 562 cells

Karel Smetana, Michaela Pluskalová, Ilona Jirásková, Zbyněk Hrkal

J Appl Biomed 3:193-198, 2005 | DOI: 10.32725/jab.2005.025

The present study was undertaken to provide more information on the nucleolar size, number and distribution of AgNORs (silver stained nucleolus organizer regions) in nucleolar bodies of aging leukemic granulocytic progenitor cells represented by cultured K562 cells. In these aging and starving cells without further differentiation and with translocated AgNORs to the nucleolar periphery, the diameter of the nucleolar size was reduced together with a reduction in the number of these nucleolar compartments. However, after re-feeding cell cultures, the nucleolar size increased again. Similarly, the number of AgNORs also increased and was distributed in...

Effect of diet supplementation with N-acetylcysteine on leucocyte functions in prematurely aging mice

Noelia Guayerbas, Marta Puerto, Carmen Alvarado, Mónica De la Fuente

J Appl Biomed 3:199-205, 2005 | DOI: 10.32725/jab.2005.026

According to the free radical theory of aging, oxidative stress plays a key role in the senescent decline of the immune system, and therefore antioxidants may provide an effective therapy in order to protect the immune function against oxidative damage. Accordingly, the purpose of this study was to investigate the favourable effect of a diet supplemented with a glutathione precursor - namely the antioxidant N-acetylcysteine (NAC, 0.1% w/w) - on several functions of the leucocytes from prematurely-aging mice (PAM). In adult PAM, the immune functions studied showed values similar to those of older mice, and worse than those found in adult non prematurely-aging...

Reviews

Endoplasmic reticulum quality control and congenital pathology

Marek Michalak

J Appl Biomed 3:159-165, 2005 | DOI: 10.32725/jab.2005.021

Quality control of the endoplasmic reticulum plays a critical role in protein folding, modification and modification of a secretory pathway. As endoplasmic reticulum chaperones, calreticulin and calnexin have similar substrate specificity and share several common features. Yet, surprisingly, mice bearing a disruption in the calreticulin gene die from a lesion in cardiac development and develop significant metabolic problems whereas calnexin-deficient mice are born alive with, yet not understood, neurological problems. Studies with calreticulin and calnexin gene knockout mice and calreticulin- and calnexin-deficient cell lines indicate that calnexin...

The nucleolar structure and nucleolar proteins as indicators of cell proliferation events in plants

Fernando González-Camacho, Francisco Javier Medina

J Appl Biomed 3:167-174, 2005 | DOI: 10.32725/jab.2005.022

Cell proliferation is a crucial cellular process which influences development. In plants, meristems are formed by actively proliferating cells, in which the main expression of proliferation is the existence of a cell division cycle. Many cell activities are influenced by the cell proliferation status and cell cycle progression, among them ribosome biogenesis, which is morphologically expressed as the nucleolus. The connection is established through nucleolar proteins, which regulate the synthesis and processing of preribosomal precursors and, at the same time, are targets of various cell cycle regulators, such as certain kinases. Nucleolin is one of...