Journal of Applied Biomedicine, 2009 (vol. 7), issue 1

Original Research Article

Protective effect of apigenin against hydrogen peroxide induced genotoxic damage on cultured human peripheral blood lymphocytes

Yasir Hasan Siddique, Mohammad Afzal

J Appl Biomed 7:35-43, 2009 | DOI: 10.32725/jab.2009.003

Apigenin is a member of the flavone family of flavonoids and possesses anti-inflammatory, free radical scavenging and anti-carcinogenic properties. Hydrogen peroxide, which is generated during oxidative stress, is known to damage proteins, nucleic acids and cell membranes and also has been implicated in cancer, ageing and several chronic neurogenerative diseases. The present study focuses on the protective effect of apigenin against genotoxic doses of hydrogen peroxide (H₂O₂) using sister chromatid exchanges (SCEs) and cytokinesis blocked micronucleus (CBMN) assay. The treatment with 50, 100 and 150 μM of H₂O₂...

Anti-lipidemic actions of essential oil extracted from Ocimum sanctum L. leaves in rats fed with high cholesterol diet

Thamolwan Suanarunsawat, Watcharaporn Devakul Na Ayutthaya, Thanapat Songsak, Jittraporn Rattanamahaphoom

J Appl Biomed 7:45-53, 2009 | DOI: 10.32725/jab.2009.004

Ocimum sanctum L. (OS) leaves have been shown to have a potential for lipid-lowering action. The present study was conducted to evaluate the anti-hyperlipidaemic ability of the EO extracted from OS leaves in rats fed with a high cholesterol (HC) diet. EO of OS leaves was extracted using the hydrodistillation method and its chemical composition was further determined by GC-MS. The results showed that phenylpropanoid compounds (eugenol and methyl eugenol) were the major components of the EO. There were no significant differences in body weight gain, food intake, and heart weight in all groups of rats. The HC diet apparently raised the serum total...

Daily rhythms of 25 physiological variables in Bos taurus maintained under natural conditions

Claudia Giannetto, Giuseppe Piccione

J Appl Biomed 7:55-61, 2009 | DOI: 10.32725/jab.2009.005

To further understanding of the multiple temporal relationships of the physiological process, we monitored simultaneously 25 different variables in individual cows. We used 6 Bruna Italiana non - pregnant and non - lactating cows from the same farm. The animals were housed individually in a 12 m2 box under natural 14/10 light/dark cycle. They were fed twice daily and water was available ad libitum. Locomotor activity and heart rate were recorded continuously. The rectal temperature, respiratory rate and blood samples were recorded every 4 hours for 24 consecutive hours. To describe the periodic phenomenon analytically we applied a trigonometric...

Reviews

Carbon nanotubes: toxicological impact on human health and environment

Gracian Tejral, Nagender Reddy Panyala, Josef Havel

J Appl Biomed 7:1-13, 2009 | DOI: 10.32725/jab.2009.001

Nanotechnology is an emerging field of science which is producing nano-sized materials. Some nanomaterials are having a significant impact in health care. Of these, carbon nanotubes (CNTs) represent one of the most promising materials in the field of nano-science and technology. Their potential in industrial applications has brought them much attention and the wide spectrum of usage has made it imperative that the impact of CNTs on human health and the environment is investigated thoroughly. In addition to their various beneficial applications, there is a potential for hazardous effects on human health. For example, the potential hazards through inhalation...

Clock genes in health and diseases

Valérie Mongrain, Nicolas Cermakian

J Appl Biomed 7:15-33, 2009 | DOI: 10.32725/jab.2009.002

Circadian rhythms, rhythms of about 24 h in various physiological functions, are getting extensively characterized at the molecular level. The generation of circadian rhythms is acknowledged to originate from oscillations in the expression of several clock genes as well as in the regulation of their protein products. While the general entrainment of organisms to the light-dark cycle of the environment is mainly achieved through the master clock of the suprachiasmatic nucleus in mammals, the molecular clockwork is functional in various organs and tissues. The molecular components of the circadian system have been found to be essential for a diversity...