Journal of Applied Biomedicine, 2011 (vol. 9), issue 1
Original Research Article
Inhibition of blood and tissue cholinesterases by soman in guinea pigs in vivo
Jiří Bajgar, Jiří Kassa, Miroslav Pohanka, Jana Žďárová Karasová, Ladislav Novotný, Josef Fusek, Václav Bláha
J Appl Biomed 9:35-41, 2011 | DOI: 10.2478/v10136-009-0030-8 
Guinea pigs were intoxicated intramuscularly with different doses of soman, and cholinesterase activities were determined in the blood, diaphragm and parts of the brain - the pontomedullar area, the frontal cortex and the basal ganglia. The time course of poisoning following low doses (1,3, 5 μg/kg) and a dose equal to 1×LD50 (28.5 μg/kg) were studied. The dose having a negligible effect on cholinesterases in the tissues studied was assessed at 1-3 μg/kg, and, following administration of a dose of 5 μg/kg, statistically significant blood cholinesterase inhibition was demonstrated.
Nycthemeral rhythms of total locomotor activity and oxidative markers in horse
Giuseppe Piccione, Claudia Giannetto, Stefania Casella, Simona Marafioti, Vanessa Messina, Anna Assenza, Caterina Faggio, Francesco Fazio
J Appl Biomed 9:43-48, 2011 | DOI: 10.2478/v10136-009-0034-4
The aim of this study was to investigate the nycthemeral rhythm of total locomotor activity (TLA) in horse and the possible involvement of the daily organization of rest/activity cycles in the fluctuation of the redox state. For this purpose we recorded TLA and determined oxidative markers in ten clinically healthy Italian Saddle horses. TLA was continuously recorded by means of an actigraphy-based data logger (Actiwatch-Mini®). For the assessment of free radicals (dROMs), the antioxidant barrier (Oxy-ads) and the thiol-antioxydant barrier (SHp), blood samples were collected every 4 hours over a 48 h period. One-way repeated measures analysis of...
Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells
Aleš Tichý, Darina Muthná, Jiřina Vávrová, Jaroslav Pejchal, Zuzana Šinkorová, Lenka Zárybnická, Martina Řezáčová
J Appl Biomed 9:49-56, 2011 | DOI: 10.2478/v10136-009-0031-7
Ionising radiation (IR) is one of the main treatment modalities in oncology. However, we still search for substances which can radio-sensitize tumour cells. In this study we used caffeine, a non-specific ataxia-telangiectasia mutated kinase (ATM) inhibitor, and studied its effect on the activation of the proteins involved in cell cycle control and the induction of apoptosis in human T-lymphocyte leukaemic MOLT-4 cells (p53 wt). We evaluated the expression of the tumour-suppressor p53 (itself and phosphorylated on Ser15 and Ser392), the cell cycle regulator p21, and the anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1). After...
Reviews
Personalized chronobiologic cybercare; other chronomics' progress by transdisciplinary cycles' congruences: season's appreciations 2009
Franz Halberg, Germaine Cornélissen, Patricia Grambsch, Rollin McCraty, Larry Beaty, Jarmila Siegelová, Pavel Homolka, Dewayne Carol Hillman, Judy Finley, Faithe Thomas, Tomoshige Kino, Miguel Revilla, Othild Schwartzkopff
J Appl Biomed 9:1-34, 2011 | DOI: 10.2478/v10136-009-0022-8
Originally a remembrance of an elderly physiologist, this paper illustrates the need for a standardized specification of certain experimental or survey conditions beyond those usually necessarily disclosed in conventional publications, namely calendar-dates, clock-times and geographic locations, to allow reference to helio-ionosphero-geomagnetics along with natural and artificial lighting and temperature. When possible, body times given by a marker rhythm also should be specified. A personalized chronobiologic cybercare can eventually include focus on infradians, beyond circadians. Benefits from longitudinal monitoring are: 1. Chronobiologically-interpreted...