Journal of Applied Biomedicine, 2012 (vol. 10), issue 3

Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

Miroslav Pohanka, Jiří Sochor, Branislav Ruttkay-Nedecký, Natalia Cernei, Vojtěch Adam, Jaromír Hubálek, Marie Stiborová, Tomáš Eckschlager, René Kizek

J Appl Biomed 10:155-167, 2012 | DOI: 10.2478/v10136-012-0006-y  

In the food industry, in the process of creating new agricultural plant products, and in the testing of anti-cancer drugs there is often a need to assay multiple samples of low molecular weight antioxidants, plant samples and foods rich in antioxidants, with minimal additional costs and low degrees of uncertainty. With these demands in mind, we decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and application of reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes. Five methods...

Non-alcoholic steatohepatitis: an overview including treatments with herbals as alternative therapeutics

Menaka Chanu Thounaojam, Ravirajsinh Navalsinh Jadeja, Ranjisinh Vijaysinh Devkar, Ayalur Vadathala Ramachandran

J Appl Biomed 10:119-136, 2012 | DOI: 10.2478/v10136-012-0008-9  

Non-alcoholic steatohepatitis (NASH), an under- recognized hepatic ailment with increasing prevalence, is fast emerging as the dark horse of hepatic related morbidity and mortality. Though introduced as a hepatic condition as early as 1980, detailed exploration of its causes, underlying mechanisms and therapeutics has largely remained an ignored or neglected field. Only recently, the focus of attention has gravitated towards an understanding of NASH as a pathological manifestation of significance and a search for possible therapeutic interventions. Treatment schedules as of now involve life style management and usage of anti-diabetic or anti-obesity...

Application of microreactors in medicine and biomedicine

Anita Šalić, Ana Tušek, Bruno Zelić

J Appl Biomed 10:137-153, 2012 | DOI: 10.2478/v10136-012-0011-1  

Microreactor technology is an interdisciplinary field that combines science and engineering. This new concept in production, analysis and research is finding increasing application in many different fields. Benefits of this new technology pose a vital influence on chemical industry, biotechnology, the pharmaceutical industry and medicine, life science, clinical and environmental diagnostic. In the last few years, together with microplant development, a great part of research investigation is focused on integrated micro-systems, the so called micro-total-analysis-systems (μ-TAS) or lab-on-chip (LOC). They are devices that perform sampling,...

Post-doctoral studies - development of new methods and evaluation of compound pharmacokinetics parameters: University Hospital Hradec Králové, Czech Republic

J Appl Biomed 10:154, 2012 | DOI: 10.1016/S1214-021X(14)60074-0  

Post-doctoral studies - position focused on synthetic work: University Hospital Hradec Králové, Czech Republic

J Appl Biomed 10:168, 2012 | DOI: 10.1016/S1214-021X(14)60076-4