Journal of Applied Biomedicine, 2014 (vol. 12), issue 3

Contents

J Appl Biomed 12:v, 2014 | DOI: 10.1016/S1214-021X(14)00062-3  

BMP-2 modulates expression of other growth factors in a rat fracture healing model

Jessica Ann Cottrell, Owen Keane, Sheldon Sutton Lin, James Patrick O'Connor

J Appl Biomed 12:127-135, 2014 | DOI: 10.1016/j.jab.2014.02.003  

Recombinant human bone morphogenetic protein-2 (rhBMP-2) is an osteo-inductive reagent that can be used therapeutically to promote spinal arthrodesis and fracture healing. Despite its clinical use, the effect of rhBMP-2 on expression of endogenous osteogenic growth factors is poorly described. The goal of this study was to determine if local rhBMP-2 treatment modulates expression of pro-osteogenic growth factors during fracture healing. Rat femur fractures were wrapped with control or rhBMP-2 collagen soaked sponge. Radiographic observations demonstrated that rhBMP-2 treatment enhanced fracture callus formation and decreased bridging time. Expression...

Combined effect of silver nanoparticles and therapeutical ultrasound on ovarian carcinoma cells A2780

Vladan Bernard, Vojtěch Mornstein, Josef Jaroš, Miroslava Sedláčková, Jiřina Škorpíková

J Appl Biomed 12:137-145, 2014 | DOI: 10.1016/j.jab.2014.01.002  

Antibacterial activity of silver nanoparticles is widely known and used. The effect of application of therapeutical ultrasonic field in the presence of silver nanoparticles

Vascular variability anomalies (VVAs) in children

Elena Vasiljevna Syutkina, Olga Viktorovna Kozhevnikova, Leila Seimurovna Namazova-Baranova, Ivan Jevgenievitch Smirnov, Anatoly Viktorovich Masalov, Germaine Cornelissen, Othild Schwartzkopff, Maria Dmitrijevna Mitish, Lyazzat Gumarova, Franz Halberg

J Appl Biomed 12:147-153, 2014 | DOI: 10.1016/j.jab.2014.02.004  

This work aims at studying in children the prevalence of some vascular variability anomalies (VVAs), i.e. indices of an increased risk of heart and vessel diseases revealed by chronobiologic analysis of cardiovascular monitoring data - systolic (SBP) and diastolic (DBP) MESOR-hypertension/hypotension, circadian hyper-amplitude-tension (CHAT), ecphasia and an excessive pulse pressure (EPP). A reference group of 138 healthy Moscow schoolchildren (age 12-17 years) was used for determination of 90% prediction limits for the circadian parameters of SBP and DBP, specified by gender and age (or height). The test group included another 194 children of the...

A comparison of the reactivating and therapeutic efficacy of two novel oximes K378 and K458 with currently available oximes in rats and mice poisoned with sarin

Jiri Kassa, Vendula Sepsova, Martina Tumova

J Appl Biomed 12:155-160, 2014 | DOI: 10.1016/j.jab.2014.01.007  

The ability of two novel oximes K378 and K348 and currently available oximes (HI-6, obidoxime) to reactivate sarin-inhibited acetylcholinesterase and to reduce acute toxicity of sarin was evaluated. Both new potential oxime reactivators were chosen for this study based on the data obtained during the extensive work on oximes development, from structure-activity relationship studies and in vitro evaluation of their ability to reactivate acetylcholinesterase inhibited by organophosphorus compounds. In vivo determined percentage of reactivation of sarin-inhibited rat blood and brain acetylcholinesterase showed that the potency of both novel...

Interaction of free radicals of DOPA-melanin-streptomycin complexes with paramagnetic oxygen O₂

Barbara K. Wiernek, Barbara Pilawa, Magdalena Zdybel, Ewa Buszman, Dorota Wrześniok

J Appl Biomed 12:161-169, 2014 | DOI: 10.1016/j.jab.2013.12.002  

Oxygen plays an important role in the metabolism of all tissues. The level of oxygen in the environment of melanin biopolymer in human body may change toxic effects resulted from free radicals formation in melanin-drug complexes.Using electron paramagnetic resonance (EPR) spectroscopy we measured the effect of molecular oxygen on concentration and properties of free radicals in 3,4-dihydroxyphenylalanine-melanin (DOPA-melanin) complexes with streptomycin. Studies have shown that free radical properties of melanin are heavily dependent on the presence of oxygen in the environment of the sample. Streptomycin increases the concentration of free radicals...

Identification and characterisation of different proteases in Lucilia sericata medicinal maggots involved in maggot debridement therapy

Ivana Valachova, Tomas Majtan, Peter Takac, Juraj Majtan

J Appl Biomed 12:171-177, 2014 | DOI: 10.1016/j.jab.2014.01.001  

Maggot debridement therapy (MDT) is increasingly being used as a fast and effective treatment approach for healing chronic wounds. The removal of necrotic tissue from wounds is a fundamental step in successful treatment. Larvae of Lucilia sericata produce a cocktail of proteolytic and antimicrobial substances in the gut as well as salivary glands called excretion/secretion (ES) products. Three groups of proteolytic enzymes (serine, aspartyl and metalloproteinases) were identified in the larval ES products to date. We prepared cDNA library from the salivary glands and identified novel putative serine proteases, metalloproteinase and signal peptide...

Evaluation of antibacterial activity of hexanedioic acid isolated from Hermetia illucens larvae

Won Hyung Choi, MeiHua Jiang

J Appl Biomed 12:179-189, 2014 | DOI: 10.1016/j.jab.2014.01.003  

Hermetia illucens larvae have been used in Europe and America as a medical resource and medicinal insect for the treatment of skin damage such as burns and wound healing. This study was carried out to evaluate the effect of a new substance causing antibacterial activity against various pathogenic bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). The antibacterial activity of hexanedioic acid was determined using different antimicrobial indicators such as turbidometric assay, resazurin assay, and agar disk diffusion. Hexanedioic acid showed the selective-growth inhibitory effects against the growth and proliferation...