Journal of Applied Biomedicine, 2023 (vol. 21), issue 3
Original Research Article
The importance of preoperative and perioperative Narrow Band Imaging endoscopy in the diagnosis of pre-tumor and tumor lesions of the larynx
Tomáš Filipovský, David Kalfeřt, Eva Lukavcová, Šárka Zavázalová, Jiří Hložek, Daniel Kovář, Jaromír Astl, Richard Holý
J Appl Biomed 21:107-112, 2023 | DOI: 10.32725/jab.2023.015 
Introduction: Narrow band imaging (NBI) is an endoscopic imaging method intended for the diagnosis of mucosal lesions of the larynx that are not visible in white-light endoscopy, but are typical of pre-tumor and tumor lesions of the larynx. The purpose of the study: To compare preoperative/perioperative white light endoscopy and NBI endoscopy with the results of histopathological examinations in pre-tumor and tumor lesions of the larynx. Methods: A prospective study, over a period of five years (5/2018-5/2023), included 87 patients with laryngeal lesions aged 24-80 years. We evaluated preoperative/ perioperative white light and NBI endoscopy, established...
Resting-state EEG alpha rhythm spectral power in children with specific language impairment: a cross-sectional study
Nina Stanojević, Saška Fatić, Ljiljana Jeličić, Vanja Nenadović, Miodrag Stokić, Ružica Bilibajkić, Miško Subotić, Tatjana Bošković Matić, Ljubica Konstantinović, Dragana Ćirović
J Appl Biomed 21:113-120, 2023 | DOI: 10.32725/jab.2023.013
Purpose: This study investigated EEG alpha rhythm spectral power in children with Specific Language Impairment (SLI) and compared it to typically developing children to better understand the electrophysiological characteristics of this disorder. Specifically, we explored resting-state EEG, because there are studies that point to it being linked to speech and language development. Methods: EEG recordings of 30 children diagnosed with specific language impairment and 30 typically developing children, aged 4.0-6.11 years, were carried out under eyes closed and eyes open conditions. Differences in alpha rhythm spectral power in relation to brain topography...
Anticancer and antimicrobial evaluation of extract from brown algae Hormophysa cuneiformis
Nehal A. H. K. Osman, Omniya M. Abd-Elazeem, Rasha A. Al-Eisa, Nahla S. El-Shenawy
J Appl Biomed 21:121-136, 2023 | DOI: 10.32725/jab.2023.016
Aim: We investigated the antimicrobial and anticancer properties of an ethanol crude extract of Red Sea brown alga (Hormophysa cuneiformis) from Egypt. Methods: Extraction was achieved by mixing 100 g of sample powder with absolute ethanol, incubating at 37 °C overnight in a shaking incubator, and then collecting the extract. The extract's antimicrobial activity was tested using a well diffusion assay against the tested pathogens (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Candida albicans) in comparison to commercial antibiotics. Anticancer activity was assessed using MTT assay on MCF-7, HepG-2, and HEP-2...
Guanxinning tablets improve myocardial hypertrophy by inhibiting the activation of MEK-ERK1/2 signaling pathway
Yan Zhang, Yu Huang, Quan-xin Ma, Song-tao Xu, Liye Shen, Yan-yun Xu, Hai-ye Tu, Min-li Chen, Yi-li Rong
J Appl Biomed 21:137-149, 2023 | DOI: 10.32725/jab.2023.014
Myocardial hypertrophy may lead to heart failure and sudden death. As traditional Chinese medicine, Guanxinning tablets (GXN) have significant pharmacological effects in the prevention and treatment of cardiovascular diseases. However, the anti-cardiac hypertrophy efficacy of GXN and its mechanism of action are still unclear. Therefore, we established a heart failure rat model and isolated primary cardiomyocytes of neonatal rat to observe the protective effect of GXN on heart failure rat model and the intervention effect on myocardial cell hypertrophy, and to explore the possible mechanism of GXN preventing and treating myocardial hypertrophy. The...
The molecular targets of Kangai injection in gastric cancer by in silico network pharmacology approach and experiment confirmation
Yongjun Qiu *, Sujun Huang, Minfang Zhu
J Appl Biomed 21:150-159, 2023 | DOI: 10.32725/jab.2023.017
Introduction: This study aimed to identify the phytochemical constituents that could target gastric cancer in Kangai injection using a network pharmacology-based approach. Methods: Protein-protein interactions (PPI), Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were conducted utilizing String and OmicShare tools. In the in vitro experiments, the related mRNA and protein levels were assessed via real-time quantitative polymerase chain reaction and Western blotting, respectively. Cell proliferation was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay. Results:...
Allele frequency and genotype distribution of the opioid receptor μ-1 (OPRM1) A118G polymorphism in the Western Saudi population
Amina M. Bagher *, Rawan H. Hareeri
J Appl Biomed 21:160-165, 2023 | DOI: 10.32725/jab.2023.012
The single nucleotide polymorphism (SNP) A118G (rs1799971) in the Mu Opioid Receptor 1 (OPRM1) gene is associated with significant variations in analgesic doses and adverse effects of opioids. The A118G OPRM1 allele distributions vary significantly between different populations worldwide. The study aimed to assess the allele frequency and genotype distribution of OPRM1 A118G SNP in Saudis. This cross-sectional study included 124 healthy Saudis (62 males and 62 females) visiting the King Abdulaziz University Hospital in Jeddah, Saudi Arabia. The Oragene®-DISCOVER (OGR-600) kits were used to collect saliva samples from the participants. Polymerase...