Journal of Applied Biomedicine, 2025 (vol. 23), issue 4
Original Research Article
Exosomes from LPS-stimulated macrophages alleviate neuroinflammatory responses by reducing pyroptosis in systemic lipopolysaccharide-induced mice
Tao Ying, Zhezhe Sun *
J Appl Biomed 23:163-173, 2025 | DOI: 10.32725/jab.2025.018 
Central nervous system (CNS) inflammation occurs in cognitive dysfunction, but the underlying mechanisms remain unclear. The newly discovered pattern of cell death in recent years is called pyroptosis, which is distinguished from apoptosis and necrosis, and is mainly dependent on caspase-1 mediated inflammatory response. Stem-derived exosomes have immunomodulatory and immunosuppressive effects. In the present study, we aimed to investigate the exosomes (Ex) secreted by lipopolysaccharide (LPS)-stimulated macrophages (LPS-Ex) to attenuate the neuroinflammatory response caused by systemic LPS stimulation by attenuating pyroptosis. We studied lipopolysaccharide...
Propofol suppresses breast cancer invasion: An in vitro three-dimensional cell invasion model with microfluidic technology
Zhitong Wan, Haoyue Lyu, Yuting Luo, Tong Liu, Xiaoyu Peng, Yijing Zhang, Yuan Li
J Appl Biomed 23:174-183, 2025 | DOI: 10.32725/jab.2025.019
The fundamental aspect of breast cancer metastasis is the infiltration of malignant cells, which can be blocked by propofol, a widely utilized anesthetic in clinical settings, as recent studies reporting. However, research utilizing three-dimensional invasion models in vitro has not been documented. This study created a microfluidic chip model utilizing type I collagen (Col1), integrating delayed dynamic imaging and several fluorescence labeling approaches to objectively assess the inhibitory effect of propofol on breast cancer cell MDA-MB-231 invasion. Research indicates that MDA-MB-231 cells demonstrate collective invasion behavior, with their...
Relationship between interleukin-37 genetic polymorphisms and HBV-related liver disease in a Chinese Han cohort
Ping Fang, Ping Meng, Lijun Du, Hui Li, Rong Wang, Juan Zhao, Decheng Cai
J Appl Biomed 23:184-196, 2025 | DOI: 10.32725/jab.2025.017
Hepatitis B virus (HBV)-related liver disease is an inflammatory-associated disease, with diverse clinical phenotypes ranging from asymptomatic HBV carriers to hepatocellular carcinoma. Interleukin-37 (IL-37), a cytokine that effectively inhibits innate and adaptive immunity, has powerful anti-inflammatory and anti-tumor effects. Several single nucleotide polymorphisms (SNPs) in the IL37 gene are genetic predictive risk factors for HBV infection and HBV-mediated liver disease progression. However, different ethnic groups may have different allele frequencies and linkage disequilibrium structures. The effect of SNPs in IL37 on HBV infection...