Journal of Applied Biomedicine, 2026 (vol. 24), issue 1
Original Research Article
miR-500a-3p negatively regulates SOCS2 and participates in the proliferation, glycolysis, and apoptosis of HCC cells via the JAK2/STAT5 pathway
Shan Li, Wei Luo, Wei Liu
J Appl Biomed 24:10-26, 2026 | DOI: 10.32725/jab.2026.002 
Background: HCC is a prevalent malignant tumor globally with high mortality. MiR-500a-3p plays critical roles in tumorigenesis and tumor progression. Methods: To evaluate miR-500a-3p's role in HCC, we first analyzed its expression and prognostic value via qRT-PCR and TCGA (Kaplan-Meier analysis). We then performed extensive in vitro functional studies after cell transfection (mimics, anti-miR, SOCS2 OE), measuring proliferation, migration, invasion, glycolytic parameters (glucose consumption, lactate, ECAR, ATP), and apoptosis. A target relationship with SOCS2 was predicted bioinformatically and confirmed by dual-luciferase assay. Using the...
Review Article
Walking the tightrope: Balancing immune checkpoints and their inhibitors in host defence against bacterial infections
Anna Palounková, Andrea Frejlachová, Anna Kovaříková, Aleš Chrdle, Helena Langhansová
J Appl Biomed 24:1-9, 2026 | DOI: 10.32725/jab.2026.001
Immune checkpoints are one of the mechanisms that maintain the balance between immunotolerance and immunopathology. These mechanisms are often exploited by tumour cells. Thanks to extensive global testing of anticancer drugs, a revolutionary cancer therapy based on immune checkpoint inhibitors (ICIs) has been developed. Some pathogens exploit regulatory checkpoint interactions, contributing to the establishment of hidden, long-term, or persistent infections in which the host is unable to eliminate the pathogen. However, a structured overview of immune checkpoint involvement in bacterial infections remains underrepresented in the current scientific...