Journal of Applied Biomedicine, 2007 (vol. 5), issue 1
Original Research Article
Convenient synthesis of 3,5-dialkoxy-4-hydroxy cinnamic acids
Young-Sik Jung, Hyang-Ok Jo
J Appl Biomed 5:13-17, 2007 | DOI: 10.32725/jab.2007.002 
3,5-Dialkoxy-4-hydroxy cinnamic acids 8a-g have been conveniently synthesized from methyl 4-benzyloxy-3,5-dihydroxybenzoate 4. In five steps, cinamic acids 8a-g having various alkoxy groups on C-3 and C-5 positions of the phenyl ring were obtained in 33-70 % overall yields. The antioxidant properties of the newly synthesized amides and the effects on lipid peroxidation in rat brain homogenate will be examined by thiobarbituric acid reactive substances (TBARS) assay and other methods.
Cell migration and the ganglioside composition of the cell lines derived from Reuber hepatoma
Lenka Dudková, Lucie Muchová, Eva Brožová, František Šmíd
J Appl Biomed 5:19-23, 2007 | DOI: 10.32725/jab.2007.003
The migration potency of differentiated (H-4-II-E, H-4-II-E-C3) and dedifferentiated (H-5) cell lines originated from Reuber hepatoma H-35, and their connection to the morphology and expression of gangliosides were examined. The migration capacity tested by a Transwell assay was fifteen times higher in H-5 cells compared to H-4-II-E cells. The ganglioside pattern was assessed by thin-layer chromatography (TLC). H-4-II-E and H-4-II-E-C3 cell lines expressed Fuc-GM1, which was not found in H-5 cells, whereas H-5 expressed GM3, which was absent in differentiated cell lines. GM3 ganglioside is thought to be one of the key molecules involved in signal transduction...
In vitro reactivation potency of bispyridinium (E)-but-2-ene linked acetylcholinesterase reactivators against tabun-inhibited acetylcholinesterase
Kamil Musílek, Kamil Kuča, Daniel Jun, Martin Doležal
J Appl Biomed 5:25-30, 2007 | DOI: 10.32725/jab.2007.004
Six potential bispyridinium (E)-but-2-ene linked AChE reactivators were tested on tabun-inhibited acetylcholinesterase in vitro. Their reactivation efficacy was compared to currently available AChE reactivators used in the case of organophosphorus intoxications - pralidoxime, HI-6 and obidoxime. According to the results obtained, (E)-1,4'-bis(4-hydroxyiminomethylpyridinium)but-2-ene dibromide (K075) seems to be the most potent reactivator of tabun-inhibited AChE. From the data, the reactivation potency of these compounds depends on structural factors such as the constitution of the linking chain between both pyridinium rings and...
Protective role of tetrahydrocurcumin on changes in the fatty acid composition in streptozotocin-nicotinamide induced type 2 diabetic rats
Pidaran Murugan, Leelavinothan Pari
J Appl Biomed 5:31-38, 2007 | DOI: 10.32725/jab.2007.005
Curcumin is the most active component of turmeric. It is believed that curcumin is a potent antioxidant and anti-inflammatory agent. Tetrahydrocurcumin (THC), one of the major metabolites of curcumin, exhibits many of the same physiological and pharmacological activities as curcumin and in some systems may exert greater antioxidant activity than curcumin. The aim of this study was to evaluate the effect of THC on the blood glucose, plasma insulin and fatty acid composition of the total lipids in the liver, kidney and brain of control and streptozotocin (STZ)-nicotinamide diabetic rats. The analysis of fatty acids showed that there was a significant...
MALDI TOF mass spectrometry of selected mycotoxins in barley
Shaban Elosta, Dagmar Gajdošová, Blanka Hégrová, Josef Havel
J Appl Biomed 5:39-47, 2007 | DOI: 10.32725/jab.2007.006
A novel method for the simultaneous determination of the trichothecene mycotoxins deoxynivalenol (DON), nivalenol (NIV), 3-acetyl-deoxynivalenol (3-ADON), and 15-acetyl-deoxynivalenol (15-ADON) in barley and malt extracts has been developed using Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI TOF MS). This technique enables highly sensitive and fast analysis and/or detection using very small samples. In this work several matrices were examined and the most suitable ones were identified. Statistical analysis was carried out to verify the ability of the system to determine the mycotoxins in a real sample. Test for...
Nucleolar proteins change in altered gravity
Margarita A. Sobol, Fernando González-Camacho, Elizabeth L. Kordyum, Francisco Javier Medina
J Appl Biomed 5:49-56, 2007 | DOI: 10.32725/jab.2007.007
Nucleolin is a major highly phosphorylated nucleolar protein involved in the regulation of r-chromatin condensation/expansion and rDNA transcription as well as in rRNA processing. The nucleolar protein homologous to the mammalian nucleolin and to the onion nucleolin-like protein NopA100, was detected in the nuclear soluble protein fraction, and in the nuclear matrix fraction from Lepidium sativum root meristematic cells, using the selective silver staining method and the cross-reaction with the anti-NopA100 antibody. In 2-DE Western blots of both nuclear fractions, the nucleolin-like protein was revealed as a smear on the level of 90 kDa extending...
Reviews
Role of tau in Alzheimer's dementia and other neurodegenerative diseases
Amita Quadros, Ophelia Inez Weeks, Ghania Ait-Ghezala
J Appl Biomed 5:1-12, 2007 | DOI: 10.32725/jab.2007.001
Alzheimer's disease (AD) is defined histopathologically by beta-amyloid (Aβ) senile plaques and neurofibrillary tangles (NFTs) composed of hyperphosphorylated tau. The question as to which of these lesions takes precedence in AD pathology has long been an issue of debate. The amyloid cascade hypothesis, currently the predominant hypothesis, considers Aβ peptide to be responsible for the major neurodegeneration observed in AD while the cytoskeleton hypothesis states that tau hyperphosphorylation and subsequent aggregation may be central to the neurodegeneration observed in AD. This review focuses on tau mutations, phosphorylation sites, tau...