J Appl Biomed 4:53-58, 2006 | DOI: 10.32725/jab.2006.004

Influence of Hibiscus sabdariffa (Gongura) on the levels of circulatory lipid peroxidation products and liver marker enzymes in experimental hyperammonemia

Musthafa Mohamed Essa, Perumal Subramanian*, Ganapathy Suthakar, Tamilarasan Manivasagam, Kadiyala Babu Dakshayani, Ramar Sivaperumal, Selvaraju Subash, Govindarajaha Vinothini
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar-608002, Tamil Nadu, India

Hibiscus sabdariffa (Linn) (family Malvaceae), is an annual dicotyledonous herbaceous shrub plant popularly known as 'Gongura' in Hindi or 'Pulicha keerai' in Tamil, which is an indigenous edible medicinal plant used in Ayurvedic Medicine in India, China and Thailand. We have investigated the influence of Hibiscus sabdariffa leaf extract (HSEt) on the levels of circulatory ammonia, urea, lipid peroxidation products such as TBARS (thiobarbituric acid and reactive substances), HP (hydroperoxides) and liver marker enzymes such as AST (aspartate transaminase), ALT (alanine transaminase) and ALP (alkaline phosphatase), for its hepatoprotective effect in ammonium chloride induced hyperammonemia. Ammonium chloride treated rats showed a significant increase in the levels of circulatory ammonia, urea, AST, ALT, ALP, TBARS and HP. These changes were significantly decreased in rats treated with HSEt and ammonium chloride. Our results indicate that HSEt offers hepatoprotection by influencing the levels of lipid peroxidation products and liver markers in experimental hyperammonemia and this could be due to its free radical scavenging property and the presence of natural antioxidants. The exact mechanism has to be still investigated and the isolation of active constituents is required.

Keywords: ammonium chloride; hyperammonemia; urea; lipid peroxidation; AST; ALT; ALP

Received: September 20, 2005; Published: March 31, 2006  Show citation

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Essa MM, Subramanian P, Suthakar G, Manivasagam T, Babu Dakshayani K, Sivaperumal R, et al.. Influence of Hibiscus sabdariffa (Gongura) on the levels of circulatory lipid peroxidation products and liver marker enzymes in experimental hyperammonemia. J Appl Biomed. 2006;4(1):53-58. doi: 10.32725/jab.2006.004.
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    References

    1. Adegunloye B.J., Omoniyi J.O., Owolabi O.A. et al.: Mechanisms of the blood pressure lowering effects of the calyx extract of Hibiscus sabdariffa in rats. Afr. J. Med. Med. Sci. 25:235-238,1996. Go to PubMed...
    2. Amin A., Hamza A.A.: Hepatoprotective effects of Hibiscus, Rosmarinus and Salvia on azathioprine-induced toxicity in rats. Life Sci. In press, 2005. Go to original source... Go to PubMed...
    3. Chifundera K., Balagizi K., Kizungu B.: Les empoisonnements et leurs antidotes en me' decine traditionnelle au Bushi, Zaire. Fitoterapia 65:307-313, 1994.
    4. Dakshayani K.B., Velvizhi S., Subramanian P.: Effects of ornithine alpha ketoglutarate on circulatory antioxidants and lipid peroxidation products in ammonium acetate treated rats. Ann. Nutr. Metab. 46:93-96, 2002a. Go to original source... Go to PubMed...
    5. Dakshayani K.B., Velvizhi S., Subramanian P.: Ornithine alpha ketoglutarate modulates the levels of antioxidants and lipid peroxidation products in ammonium acetate treated rats. Indian J. Biochem. Biophys. 36:422-424, 2002b.
    6. Devipriya S., Shyamaladevi C.S.: Protective effect of quercetin in cisplastin induced cell injury in the rat kidney, Indian J. Pharmacol. 31:422-427, 1999.
    7. Duh P.D., Yed G.C.: Antioxidative activity of three herbal water extracts. Food Chemistry 60:639-645, 1997. Go to original source...
    8. Essa M.M., Subramanian P., Suthakar G. et al.: Protective influence of Pongamia pinnata (Karanja) on blood ammonia and urea levels in ammonium chloride-induced hyperammonemia: antihyper-ammonemic effect of the leaf extract. J. Appl. Biomed. 3:133-138.2005. Go to original source...
    9. Hilgier W., Albrecht J., Lisy V., Stastny F.: The effect of acute and repeated hyperammonemia on gamma-glutamyl transpeptidase in homogenates and capillaries of various rat brain regions. Mol. Chem. Neuropathol. 13:47-56, 1994. Go to original source... Go to PubMed...
    10. Jiang Z.Y, Hunt J.V., Wolff S.P.: Detection of lipid hydroperoxides using the Fox reagent. Ann. Biochem. 202:384-389, 1992. Go to original source... Go to PubMed...
    11. King E., Armstrong A.R.: Determination of serum and bile phosphatase activity. Canad. Med. Assoc. J. 31:376-378, 1934
    12. Kosenko E., Kaminsky M., Kaminsky A. et al.: Superoxide production and antioxidant enzymes in ammonia intoxication in rats. Free Radic. Res. 27:637-644, 1997. Go to original source... Go to PubMed...
    13. Kosenko E., Kaminsky Y., Stavroskaya I.G., Felipo V.: Alteration of mitochondrial calcium homeostasis by ammonia-reduced activation of NMDA receptors in rat brain in vivo. Brain Res. 880:139-146, 2000. Go to original source... Go to PubMed...
    14. Lena P.J., Subramanian P.: Evaluation of the antiperoxidative effects of melatonin in ammonium acetate-treated Wistar rats. Pol. J. Pharmacol. 55:1031-1036, 2003.
    15. Lena P.J., Subramanian P.: Effects of melatonin on the levels of antioxidants and lipid peroxidation products in rats treated with ammonium acetate. Pharmazie 59:636-639, 2004. Go to PubMed...
    16. Lewis Y.S., Neelakantan S.: Hydroxycitric acid: the principal acid in the fruits of Garcinia cambogia Desr. Phytochemistry 4:619-625, 1965. Go to original source...
    17. Liu C., Wang J., Chu C. et al.: In vivo protective effect of protocatechuic acid on tert-butyl hydroperoxide-induced rat hepatotoxicity. Food Chem. Toxicol. 40:635-641, 2002. Go to original source... Go to PubMed...
    18. Majeed K.I.: Hyperammonemia is associated with an increase in inhibitory neurotransmission as a consequence of two factors. E. Med J. 2:12-15, 2005.
    19. Mathias R.S., Kostiner D., Packman S.: Hyperammonemia in urea cycle disorders: role of the nephrologist. Am. J. Kidney Dis. 37:1069-1080, 2001. Go to original source... Go to PubMed...
    20. Meyer A.S., Yi O.S., Pearson D.A. et al.: Inhibition of human low-density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). J. Agric. Food Chem. 45:1638-1643, 1997. Go to original source...
    21. Murthy C.R., Rama Rao K.V., Bai G., Norenburg M.D.: Ammonia induced production of free radicals in primary cultures of rat astrocytes. J. Neurosci. Res. 66:282-288, 2001. Go to original source... Go to PubMed...
    22. Nelson D.L., Cox M.M.: Lehninger Principles of Biochemistry. Macmillan, London 2000, pp. 439-459.
    23. Niehaus W.G., Samuelson B.: Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur. J. Biochem. 6:126-130, 1968. Go to original source... Go to PubMed...
    24. Obiefuna P.C.M., Owolabi O.A., Adegunloye B.J. et al.: The petal extract of Hibiscus sabdariffa produces relaxation of isolated rat aorta. Int. J. Pharmacognosy 32:69-74, 1993. Go to original source...
    25. Odigie I.P., Ettarh R.R., Adigun S.A.: Chronic administration of aqueous extract of Hibiscus sabdariffa attenuates hypertension and reverses cardiac hypertrophy in 2K-1C hypertensive rats. J. Ethnopharmacol. 86:181-185, 2003. Go to original source... Go to PubMed...
    26. Osman A.M., El-Garby Younes M., Mokhtar A.: Sitosterol - galactoside from Hibiscus sabdariffa. Phytochemistry 14:829-830, 1975. Go to original source...
    27. Reitman S., Frankel A.S.: A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28:56-63, 1957. Go to original source... Go to PubMed...
    28. Rodrigo R., Montoliu C., Chatauret N. et al.: Alterations in soluble guanylate cyclase content and modulation by nitric oxide in liver disease. Neurochem. Int.. 45:947-953, 2004. Go to original source... Go to PubMed...
    29. Saez R., Liansola M., Felipo V.: Chronic exposure to ammonia alters pathways modulating phosphorylation of microtubule associated protein 2 in cerebellar neurons in culture. J. Neurochem. 73:2555-2562, 1999. Go to original source... Go to PubMed...
    30. Satue-Gracia M.T., Heinonen M., Frankel E.N.: Anthocyanins as antioxidants on human low-density lipoprotein and lecithin - liposome systems. J. Agric. Food. Chem. 45:3362-3367, 1997. Go to original source...
    31. Schliess F., Gorg B., Fischer R. et al.: Ammonia induces MK-801-sensitive nitration and phosphorylation of protein tyrosine and residues in rat astrocytes. FASEB J. 16:739-741, 2002. Go to original source... Go to PubMed...
    32. Seca A.M.L., Silva A.M.S., Silvestre A.J.D. et al.: Lignanamides and other phenolic constituents from the bark of kenaf (Hibiscus cannabinus). Phytochemistry 58:1219-1223, 2000. Go to original source...
    33. Seca A.M.L., Silva A.M.S., Silvestre A.J.D. et al.: Phenolic constituents from the core of kenaf (Hibiscus cannabinus). Phytochemistry. 56:759-767, 2001. Go to original source... Go to PubMed...
    34. Shirwaikar A., Malini S., Kumari S.C.: Protective effect of Pongamia pinnata flowers against cisplatin and gentamicin induced nephrotoxicity in rats. Indian J. Exp. Biol. 1:58-62, 2003.
    35. Srinivasan K., Muruganandan S., Lal J. et al.: Evaluation of anti-inflammatory activity of Pongamia pinnata leaves in rats. J Ethnopharmacol. 78:151-157, 2001. Go to original source... Go to PubMed...
    36. Tream W.R.: Inherited and acquired syndromes of hyperammonemia and encephalopathy in children. Semin. Liver Dis. 14:236-258, 1994. Go to original source... Go to PubMed...
    37. Trease C.E., Evan V.C.: Pharmacopoeial and related drugs of biological origin. Part V Pharmacognosy. Saunders, London 1959, pp. 161-466, 1959.
    38. Tsai P.J., McIntosh J., Pearce P. et al.: Anthocyanin and antioxidant capacity in Roselle (Hibiscus Sabdariffa L.) extract. Food Res. Int.. 35:351-356, 2002. Go to original source...
    39. Tseng T.H., Wang C.J., Kao E.S., Chu H.Y.: Hibiscus protocatechuic acid protects against oxidative damage induced by tert-butylhydroperoxide in rat primary hepatocytes. Chem.Biol. Interact. 101:137-148, 1996. Go to original source... Go to PubMed...
    40. Varley H., Gowenlock A.H., Bell M.: Practical Clinical Biochemistry, 4th edn. CBS Publishers, New Delhi 2004, pp. 161-210.
    41. Velvizhi S., Dakshayani K.B., Subramanian P.: Effects of alpha-ketoglutarate on antioxidants and lipid peroxidation in rats treated with ammonium acetate. Nutrition 18:747-750, 2002 a. Go to original source... Go to PubMed...
    42. Velvizhi S., Dakshayani K.B., Subramanian P.: Protective influences of alpha-ketoglutarate on lipid peroxidation and antioxidant status ammonium acetate rats. Indian J. Exp. Biol. 40:1183-1186, 2002b.
    43. Vidhya M., Subramanian P.: Enhancement of circulatory antioxidants by alpha-ketoglutarate during sodium valproate treatment in Wistar rats. Pol. J. Pharmacol. 55:31-36, 2003.
    44. Wang C.J., Wang J.M., Lin W.L. et al.: Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food Chem. Toxicol. 38:411-416, 2000. Go to original source... Go to PubMed...
    45. Wang H., Cao G., Prior R.L.: Oxygen radical absorbing capacity of anthocyanins. J Agric. Food Chem. 45:302-309, 1997. Go to original source...
    46. Wolheim D.F.: Preanalytical increase of ammonia in blood specimens from healthy subjects. Clin. Chem. 30:906-908, 1984.

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