J Appl Biomed 4:147-151, 2006 | DOI: 10.32725/jab.2006.016

Reactivation of acetycholinesterase inhibited by the pesticide chlorpyrifos

Veronika Račáková1, Daniel Jun2, Veronika Opletalová1, Kamil Kuča2,*
1 Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic
2 Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic

Organophosphorus pesticides such as parathion or chlorpyrifos are substances used worldwide for agricultural purposes. These compounds are able to inhibit an enzyme acetylcholinesterase (AChE; EC 3.1.1.7) by phosphorylation in its active site. AChE reactivators and anticholinergics are generally used as antidotes in the case of intoxication by these agents. In this work, the reactivation potency of nine structurally different AChE reactivators was tested in vitro. Chlorpyrifos was chosen as an appropriate member of the pesticide family. The result is that bisquaternary reactivators with two oxime groups in position four at the pyridinium rings (trimedoxime and K074) seem to be the most potent reactivators of chlorpyrifos-inhibited AChE.

Keywords: acetylcholinesterase; reactivator; pesticide; organophosphate

Received: January 4, 2006; Revised: March 2, 2006; Published: July 31, 2006  Show citation

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Račáková V, Jun D, Opletalová V, Kuča K. Reactivation of acetycholinesterase inhibited by the pesticide chlorpyrifos. J Appl Biomed. 2006;4(3):147-151. doi: 10.32725/jab.2006.016.
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